ASCC2

ASCC2
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2DI0
Identifikatori
Aliasi	ASCC2
Vanjski ID-jevi	OMIM: 614216 MGI: 1922702 HomoloGene: 41774 GeneCards: ASCC2
Lokacija gena (čovjek)
Hrom.	Hromosom 22 (čovjek)
Kraj	29,838,304 bp
Lokacija gena (miš)
Hrom.	Hromosom 11 (miš)
Kraj	4,635,699 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ortolozi
	84164
	75452
	ENSG00000100325
	ENSMUSG00000020412
	Q9H1I8
	Q91WR3
	NM_001242906; NM_032204
	NM_029291; NM_001376980
NP_001229835; NP_115580; NP_001356849; NP_001356850; NP_001356851;
	NP_001356852; NP_001356853; NP_001356854; NP_001356855; NP_001356856; NP_001356857; NP_001356858; NP_001356859; NP_001356860; NP_001356861; NP_001356862; NP_001356863; NP_001356864; NP_001356865; NP_001356866; NP_001356867; NP_001356868; NP_001356869; NP_001356870; NP_001356871; NP_001356872; NP_001356873; NP_001356874; NP_001356875; NP_001356876; NP_001356877; NP_001356878; NP_001356879
	NP_083567; NP_001363909
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Podjedinica 2 kompleksa aktivirajućeg signalnog kointegratora 1 jest protein koji je kod ljudi kodiran genom ASCC2 sa hromosoma 22.^[5]^[6]^[7]

U jednom istraživanju iz 1994., osmišljen je smo metod za zamjenu strukture kapice iRNK sa oligoribonukleotidom (r-oligo) za obilježavanje 5' kraja eukariotske iRNK. Metod se sastoji od uklanjanja poklopca pirofosfatazom nikotinske kiseline (TAP) i ligiranja r-oligoribonukleotida na dekapirane iRNK sa T4 RNK-ligazom. Ova reakcija je napravljena za kap-specifičnu uklanjanjem 5'-fosfata nekapiranih RNK sa alkalnom fosfatazom prije TAP tretmana. Za razliku od konvencijskih metoda koji označavaju 5' kraj cDNK, ovaj postupak posebno označava zatvoren kraj iRNK sa sintetskim r-oligoribonukleotida, prije sinteze prvog lanca cDNK. 5' kraj iRNK je vrlo jednostavno identificiran lančanom reakcijom reverzne polimerazne transkripcije (RT-PCR).^[8]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 757 aminokiselina, a molekulska težina 86.360 Da.^[9]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MPALPLDQLQ	ITHKDPKTGK	LRTSPALHPE	QKADRYFVLY	KPPPKDNIPA
LVEEYLERAT	FVANDLDWLL	ALPHDKFWCQ	VIFDETLQKC	LDSYLRYVPR
KFDEGVASAP	EVVDMQKRLH	RSVFLTFLRM	STHKESKDHF	ISPSAFGEIL
YNNFLFDIPK	ILDLCVLFGK	GNSPLLQKMI	GNIFTQQPSY	YSDLDETLPT
ILQVFSNILQ	HCGLQGDGAN	TTPQKLEERG	RLTPSDMPLL	ELKDIVLYLC
DTCTTLWAFL	DIFPLACQTF	QKHDFCYRLA	SFYEAAIPEM	ESAIKKRRLE
DSKLLGDLWQ	RLSHSRKKLM	EIFHIILNQI	CLLPILESSC	DNIQGFIEEF
LQIFSSLLQE	KRFLRDYDAL	FPVAEDISLL	QQASSVLDET	RTAYILQAVE
SAWEGVDRRK	ATDAKDPSVI	EEPNGEPNGV	TVTAEAVSQA	SSHPENSEEE
ECMGAAAAVG	PAMCGVELDS	LISQVKDLLP	DLGEGFILAC	LEYYHYDPEQ
VINNILEERL	APTLSQLDRN	LDREMKPDPT	PLLTSRHNVF	QNDEFDVFSR
DSVDLSRVHK	GKSTRKEENT	RSLLNDKRAV	AAQRQRYEQY	SVVVEEVPLQ
PGESLPYHSV	YYEDEYDDTY	DGNQVGANDA	DSDDELISRR	PFTIPQVLRT
KVPREGQEED	DDDEEDDADE	EAPKPDHFVQ	DPAVLREKAE	ARRMAFLAKK
GYRHDSSTAV	AGSPRGHGQS	RETTQERRKK	EANKATRANH	NRRTMADRKR
SKGMIPS

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000100325 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000020412 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Jung DJ, Sung HS, Goo YW, Lee HM, Park OK, Jung SY, Lim J, Kim HJ, Lee SK, Kim TS, Lee JW, Lee YC (2002). "Novel transcription coactivator complex containing activating signal cointegrator 1". Mol. Cell. Biol. 22 (14): 5203–11. doi:10.1128/MCB.22.14.5203-5211.2002. PMC 139772. PMID 12077347.
^ Sanger Centre, The; Washington University Genome Sequencing Cente, The (1998). "Toward a complete human genome sequence". Genome Res. 8 (11): 1097–108. doi:10.1101/gr.8.11.1097. PMID 9847074.
^ "Entrez Gene: ASCC2 activating signal cointegrator 1 complex subunit 2".
^ Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
^ "UniProt, Q9H1I8" (jezik: engleski). Pristupljeno 04-02-2022. Provjerite vrijednost datuma u parametru: |access-date= (pomoć)

Dopunska literatura[uredi | uredi izvor]

Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP (1999). "The DNA sequence of human chromosome 22". Nature. 402 (6761): 489–95. doi:10.1038/990031. PMID 10591208.
Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
Carroll JS, Liu XS, Brodsky AS, Li W, Meyer CA, Szary AJ, Eeckhoute J, Shao W, Hestermann EV, Geistlinger TR, Fox EA, Silver PA, Brown M (2005). "Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1". Cell. 122 (1): 33–43. doi:10.1016/j.cell.2005.05.008. PMID 16009131.
Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070.

Vanjski linkovi[uredi | uredi izvor]

Lokacija ljudskog genoma ASCC2 i stranica sa detaljima o genu ASCC2 u UCSC Genome Browseru.
Q9H1I8

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000100325 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000020412 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid12077347-5] Jung DJ, Sung HS, Goo YW, Lee HM, Park OK, Jung SY, Lim J, Kim HJ, Lee SK, Kim TS, Lee JW, Lee YC (2002). "Novel transcription coactivator complex containing activating signal cointegrator 1". Mol. Cell. Biol. 22 (14): 5203–11. doi:10.1128/MCB.22.14.5203-5211.2002. PMC 139772. PMID 12077347.

[pmid9847074-6] Sanger Centre, The; Washington University Genome Sequencing Cente, The (1998). "Toward a complete human genome sequence". Genome Res. 8 (11): 1097–108. doi:10.1101/gr.8.11.1097. PMID 9847074.

[entrez-7] "Entrez Gene: ASCC2 activating signal cointegrator 1 complex subunit 2".

[8] Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.

[UniProt-9] "UniProt, Q9H1I8" (jezik: engleski). Pristupljeno 04-02-2022. Provjerite vrijednost datuma u parametru: |access-date= (pomoć)

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