CMPK

CMPK
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	1TEV
Identifikatori
Aliasi	CMPK1
Vanjski ID-jevi	OMIM: 191710 MGI: 1913838 HomoloGene: 32308 GeneCards: CMPK1
EC broj	2.7.4.6
Lokacija gena (čovjek)
Hrom.	Hromosom 1 (čovjek)
Kraj	47,378,839 bp
Lokacija gena (miš)
Hrom.	Hromosom 4 (miš)
Kraj	114,844,438 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• aktivnost sa transferazom; • nucleotide binding; • uridylate kinase activity; • kinase activity; • nucleobase-containing compound kinase activity; • uridine kinase activity; • nucleoside diphosphate kinase activity; • ATP binding; • cytidylate kinase activity; • nucleoside monophosphate kinase activity;
Ćelijska komponenta	• citoplazma; • citosol; • Egzosom; • jedro; • Jedarce;
Biološki proces	• nucleobase-containing small molecule interconversion; • Fosforilacija; • nucleoside diphosphate phosphorylation; • pyrimidine ribonucleotide biosynthetic process; • pyrimidine nucleotide biosynthetic process; • 'de novo' pyrimidine nucleobase biosynthetic process; • nucleoside triphosphate biosynthetic process; • nucleobase-containing compound metabolic process; • nucleoside monophosphate phosphorylation; • UMP biosynthetic process;
	Izvori:Amigo / QuickGO
Ortolozi
	51727
	66588
	ENSG00000162368
	ENSMUSG00000028719
	P30085
	Q9DBP5
	NM_001136140; NM_016308; NM_001366135
	NM_025647
	NP_001129612; NP_057392; NP_001353064
	NP_079923
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

UMP-CMP kinaza jest enzim koji je kod ljudi kodiran genom CMPK1 sa hromosoma 1.^[5]^[6]

Amiokiselininska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 196 aminokiselina, a molekulska težina 22.222 Da.^[7]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MKPLVVFVLG	GPGAGKGTQC	ARIVEKYGYT	HLSAGELLRD	ERKNPDSQYG
ELIEKYIKEG	KIVPVEITIS	LLKREMDQTM	AANAQKNKFL	IDGFPRNQDN
LQGWNKTMDG	KADVSFVLFF	DCNNEICIER	CLERGKSSGR	SDDNRESLEK
RIQTYLQSTK	PIIDLYEEMG	KVKKIDASKS	VDEVFDEVVQ	IFDKEG

Funkcija[uredi | uredi izvor]

Uridin-monofosfat (UMP)/citidin monofosfat (CMP) kinaza (EC 2.7.4.4) katalizira transfer fosforilaciju sa ATP-a na UMP, CMP i deoksi-CMP (dCMP), što rezultira stvaranjem ADP-a i odgovarajućeg nukleozid-difosfata. Ovi nukleozid-difosfati potrebni su za sintezu ćelijske nukleinske kiseline.^[6]^[8]

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000162368 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000028719 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Van Rompay AR, Johansson M, Karlsson A (1999). "Phosphorylation of deoxycytidine analog monophosphates by UMP-CMP kinase: molecular characterization of the human enzyme". Molecular Pharmacology. 56 (3): 562–9. doi:10.1124/mol.56.3.562. PMID 10462544.
^ ^a ^b EntrezGene 51727
^ "UniProt, P30085" (jezik: engleski). Pristupljeno 23. 11. 2021.
^ Liou JY, Dutschman GE, Lam W, Jiang Z, Cheng YC (2002). "Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates". Cancer Research. 62 (6): 1624–31. PMID 11912132.

Dopunska literatura[uredi | uredi izvor]

Hochstrasser DF, Frutiger S, Paquet N, Bairoch A, Ravier F, Pasquali C, Sanchez JC, Tissot JD, Bjellqvist B, Vargas R (1992). "Human liver protein map: A reference database established by microsequencing and gel comparison". Electrophoresis. 13 (12): 992–1001. doi:10.1002/elps.11501301201. PMID 1286669. S2CID 23518983.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Hughes GJ, Frutiger S, Paquet N, Pasquali C, Sanchez JC, Tissot JD, Bairoch A, Appel RD, Hochstrasser DF (1993). "Human liver protein map: Update 1993". Electrophoresis. 14 (11): 1216–22. doi:10.1002/elps.11501401181. PMID 8313870. S2CID 33424554.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5′-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL (2000). "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning". Proceedings of the National Academy of Sciences. 97 (17): 9543–8. Bibcode:2000PNAS...97.9543H. doi:10.1073/pnas.160270997. PMC 16901. PMID 10931946.
Pearman AT, Castro-Faria-Neto HC, McIntyre TM, Prescott SM, Stafforini DM (2001). "Characterization of human UMP-CMP kinase enzymatic activity and 5′ untranslated region". Life Sciences. 69 (20): 2361–70. doi:10.1016/S0024-3205(01)01322-4. PMID 11681623.
Liou JY, Dutschman GE, Lam W, Jiang Z, Cheng YC (2002). "Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates". Cancer Research. 62 (6): 1624–31. PMID 11912132.
Pasti C, Gallois-Montbrun S, Munier-Lehmann H, Veron M, Gilles AM, Deville-Bonne D (2003). "Reaction of human UMP-CMP kinase with natural and analog substrates". European Journal of Biochemistry. 270 (8): 1784–90. doi:10.1046/j.1432-1033.2003.03537.x. PMID 12694191.
Segura-Peña D, Sekulic N, Ort S, Konrad M, Lavie A (2004). "Substrate-induced Conformational Changes in Human UMP/CMP Kinase". Journal of Biological Chemistry. 279 (32): 33882–9. doi:10.1074/jbc.M401989200. PMID 15163660.
Hsu CH, Liou JY, Dutschman GE, Cheng YC (2004). "Phosphorylation of Cytidine, Deoxycytidine, and Their Analog Monophosphates by Human UMP/CMP Kinase is Differentially Regulated by ATP and Magnesium". Molecular Pharmacology. 67 (3): 806–14. doi:10.1124/mol.104.006098. PMID 15550676. S2CID 579635.
Wickham L, Benjannet S, Marcinkiewicz E, Chretien M, Seidah NG (2005). "Beta-Amyloid protein converting enzyme 1 and brain-specific type II membrane protein BRI3: binding partners processed by furin". Journal of Neurochemistry. 92 (1): 93–102. doi:10.1111/j.1471-4159.2004.02840.x. PMID 15606899.
Topalis D, Kumamoto H, Amaya Velasco MF, Dugué L, Haouz A, Alexandre JA, Gallois-Montbrun S, Alzari PM, Pochet S, Agrofoglio LA, Deville-Bonne D (2007). "Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives – a screening based on affinity for the UMP-CMP binding site". FEBS Journal. 274 (14): 3704–14. doi:10.1111/j.1742-4658.2007.05902.x. PMID 17608725.
Gagyi C, Bucurenci N, Sîrbu O, Labesse G, Ionescu M, Ofiteru A, Assairi L, Landais S, Danchin A, Bârzu O, Gilles AM (2003). "UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity". European Journal of Biochemistry. 270 (15): 3196–204. doi:10.1046/j.1432-1033.2003.03702.x. PMID 12869195.
Ofiteru A, Bucurenci N, Alexov E, Bertrand T, Briozzo P, Munier-Lehmann H, Gilles AM (2007). "Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase". The FEBS Journal. 274 (13): 3363–73. doi:10.1111/j.1742-4658.2007.05870.x. PMID 17542990. S2CID 2394831.

Vanjski linkovi[uredi | uredi izvor]

Lokacija ljudskog genoma CMPK1 i stranica sa detaljima o genu CMPK1 u UCSC Genome Browseru.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000162368 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000028719 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10462544-5] Van Rompay AR, Johansson M, Karlsson A (1999). "Phosphorylation of deoxycytidine analog monophosphates by UMP-CMP kinase: molecular characterization of the human enzyme". Molecular Pharmacology. 56 (3): 562–9. doi:10.1124/mol.56.3.562. PMID 10462544.

[entrez-6] EntrezGene 51727

[UniProt-7] "UniProt, P30085" (jezik: engleski). Pristupljeno 23. 11. 2021.

[8] Liou JY, Dutschman GE, Lam W, Jiang Z, Cheng YC (2002). "Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates". Cancer Research. 62 (6): 1624–31. PMID 11912132.

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p r u Enzimi
Teme	Aktivno mjesto Alosterna regulacija Difuzijski limitirani enzim EC broj Enzimska kataliza Inhibicija enzimskih reakcija Kinetika enzima Koenzim Kooperativnost Lineweaver–Burkov dijagram Michaelis–Mentenova kinetika Mjesto vezanja Spisak enzima
Tipovi	EC1 Oksidoreduktaze/spisak EC2 Transferaze/spisak EC3 Hidrolaze/spisak EC4 Lijaze/spisak EC5 Izomeraze/spisak EC6 Ligaze/spisak