CRIP2

CRIP2
Dostupne strukture
PDB	Pretraga Human UniProta: PDBe RCSB
Spisak PDB ID kodova
	2CU8
Identifikatori
Aliasi	CRIP2
Vanjski ID-jevi	OMIM: 601183 HomoloGene: 135666 GeneCards: CRIP2
Lokacija gena (čovjek)
Hrom.	Hromosom 14 (čovjek)
Kraj	105,480,162 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• vezivanje iona metala; • vezivanje iona cinka; • GO:0001948, GO:0016582 vezivanje za proteine;
Ćelijska komponenta	• cell cortex;
Biološki proces	• positive regulation of cell population proliferation; • Hematopoeza;
	Izvori:Amigo / QuickGO
Ortolozi
	1397
	n/a
	ENSG00000182809
	n/a
	P52943
	n/a
	NM_001270837; NM_001270841; NM_001312
	n/a
	NP_001257766; NP_001257770; NP_001303
	n/a
	Wikipodaci
Pogledaj/uredi – čovjek

Cisteinom bogati protein 2 je protein koji je kod ljudi kodiran genom CRIP2.^[3]^[4]^[5]^[6]

Tsui et al. (1996) locirali su gen CRIP2 na hromosomsku poziciju 14q32, primjenom fluorescentne in situ hibridizacije (FISH).^[7] Analizom somatskih hibridnih ćelija i FISH-metoda, Karim et al. (1996) mapirali su gen CRIP2 na poziciji 14q32.3.^[8] CRIP2 i usko povezani CRIP1 su proteini bogati cisteinom i sadrže po dva LIM-domena. Visoko su eksprimirani tokom kardiovaskularnog razvoja i djeluju na premošćavanje faktora serumskog odgovora i GATA i stimuliraju ciljne gene glatkih mišića.^[9]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 208 aminokiselina, a molekulska težina 22.493 Da.^[10].

10	20	30	40	50
MASKCPKCDK	TVYFAEKVSS	LGKDWHKFCL	KCERCSKTLT	PGGHAEHDGK
PFCHKPCYAT	LFGPKGVNIG	GAGSYIYEKP	LAEGPQVTGP	IEVPAARAEE
RKASGPPKGP	SRASSVTTFT	GEPNTCPRCS	KKVYFAEKVT	SLGKDWHRPC
LRCERCGKTL	TPGGHAEHDG	QPYCHKPCYG	ILFGPKGVNT	GAVGSYIYDR
DPEGKVQP

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija[uredi | uredi izvor]

Skriningom niske strogosti ljudske biblioteke cDNK tankog crijeva cDNK bogatim cisteinom (CRIP1) cDNK, gorepomenuti Karim et al (1996) izolirali su cDNK koja kodira CRIP2, koju su nazvali ESP1/CRP2. cDNK predviđa protein od 208 aminokiselina koji sadrži dva LIM-domena. CRIP2 dijeli 93% identiteta aminokiselinske sekvence sa svojim homologom kod pacova, zvanim Esp1 ili Crp2. Northern blot analiza pokazala je široko rasprostranjenu tkivnu ekspresiju CRIP2 iRNK od 1,3 kb, s najvišim nivoom ekspresije u srcu. Također otkrivena je o druga kRNK u sjemenicima, od 1,7 kb.

Tsui et al. (1996) klonirali su cDNK ljudskog srca koja kodira CRIP2 i nazvali je CRP2. CRIP2 je 38%, odnosno 35% identičan proteinima CSRP1 i CSRP3.

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000182809 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Tsui SK, Chan PP, Cheuk CW, Liew CC, Waye MM, Fung KP, Lee CY (Feb 1997). "A novel cDNA encoding for a LIM domain protein located at human chromosome 14q32 as a candidate for leukemic translocation". Biochem Mol Biol Int. 39 (4): 747–54. PMID 8843343.
^ Karim MA, Ohta K, Egashira M, Jinno Y, Niikawa N, Matsuda I, Indo Y (januar 1996). "Human ESP1/CRP2, a member of the LIM domain protein family: characterization of the cDNA and assignment of the gene locus to chromosome 14q32.3". Genomics. 31 (2): 167–76. doi:10.1006/geno.1996.0028. PMID 8824798.
^ Huber A, Neuhuber WL, Klugbauer N, Ruth P, Allescher HD (Mar 2000). "Cysteine-rich protein 2, a novel substrate for cGMP kinase I in enteric neurons and intestinal smooth muscle". J Biol Chem. 275 (8): 5504–11. doi:10.1074/jbc.275.8.5504. PMID 10681529.
^ "Entrez Gene: CRIP2 cysteine-rich protein 2".
^ Tsui, S. K. W., Chan, P. P. K., Cheuk, C. W., Liew, C. C., Waye, M. M. Y., Fung, K. P., Lee, C. Y. A novel cDNA encoding for a LIM domain protein located at human chromosome 14q32 as a candidate for leukemic translocation. Biochem. Molec. Biol. Int. 39: 747-754, 1996. PubMed: 8843343
^ Karim, M. A., Ohta, K., Egashira, M., Jinno, Y., Niikawa, N., Matsuda, I., Indo, Y. Human ESP1/CRP2, a member of the LIM domain protein family: characterization of the cDNA and assignment of the gene locus to chromosome 14q32.3. Genomics 31: 167-176, 1996. PubMed: 8824798
^ Chang DF, Belaguli NS, Iyer D, Roberts WB, Wu SP, Dong XR, Marx JG, Moore MS, Beckerle MC, Majesky MW, Schwartz RJ (januar 2003). "Cysteine-rich LIM-only proteins CRP1 and CRP2 are potent smooth muscle differentiation cofactors". Dev. Cell. 4 (1): 107–18. doi:10.1016/S1534-5807(02)00396-9. PMID 12530967.
^ "UniProt, P52943". Pristupljeno 22. 7. 2021.

Dopunska literatura[uredi | uredi izvor]

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
van Ham M, Croes H, Schepens J, et al. (2004). "Cloning and characterization of mCRIP2, a mouse LIM-only protein that interacts with PDZ domain IV of PTP-BL". Genes Cells. 8 (7): 631–44. doi:10.1046/j.1365-2443.2003.00660.x. PMID 12839623.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569.

Vanjski linkovi[uredi | uredi izvor]

Lokacija ljudskog genoma CRIP2 i stranica sa detaljima o genu CRIP2 u UCSC Genome Browseru.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000182809 - Ensembl, maj 2017

[2] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid8843343-3] Tsui SK, Chan PP, Cheuk CW, Liew CC, Waye MM, Fung KP, Lee CY (Feb 1997). "A novel cDNA encoding for a LIM domain protein located at human chromosome 14q32 as a candidate for leukemic translocation". Biochem Mol Biol Int. 39 (4): 747–54. PMID 8843343.

[pmid8824798-4] Karim MA, Ohta K, Egashira M, Jinno Y, Niikawa N, Matsuda I, Indo Y (januar 1996). "Human ESP1/CRP2, a member of the LIM domain protein family: characterization of the cDNA and assignment of the gene locus to chromosome 14q32.3". Genomics. 31 (2): 167–76. doi:10.1006/geno.1996.0028. PMID 8824798.

[pmid10681529-5] Huber A, Neuhuber WL, Klugbauer N, Ruth P, Allescher HD (Mar 2000). "Cysteine-rich protein 2, a novel substrate for cGMP kinase I in enteric neurons and intestinal smooth muscle". J Biol Chem. 275 (8): 5504–11. doi:10.1074/jbc.275.8.5504. PMID 10681529.

[entrez-6] "Entrez Gene: CRIP2 cysteine-rich protein 2".

[7] Tsui, S. K. W., Chan, P. P. K., Cheuk, C. W., Liew, C. C., Waye, M. M. Y., Fung, K. P., Lee, C. Y. A novel cDNA encoding for a LIM domain protein located at human chromosome 14q32 as a candidate for leukemic translocation. Biochem. Molec. Biol. Int. 39: 747-754, 1996. PubMed: 8843343

[8] Karim, M. A., Ohta, K., Egashira, M., Jinno, Y., Niikawa, N., Matsuda, I., Indo, Y. Human ESP1/CRP2, a member of the LIM domain protein family: characterization of the cDNA and assignment of the gene locus to chromosome 14q32.3. Genomics 31: 167-176, 1996. PubMed: 8824798

[pmid12530967-9] Chang DF, Belaguli NS, Iyer D, Roberts WB, Wu SP, Dong XR, Marx JG, Moore MS, Beckerle MC, Majesky MW, Schwartz RJ (januar 2003). "Cysteine-rich LIM-only proteins CRP1 and CRP2 are potent smooth muscle differentiation cofactors". Dev. Cell. 4 (1): 107–18. doi:10.1016/S1534-5807(02)00396-9. PMID 12530967.

[UniProt-10] "UniProt, P52943". Pristupljeno 22. 7. 2021.

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