CROT

CROT
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	1XL7, 1XL8, 1XMC, 1XMD
Identifikatori
Aliasi	CROT
Vanjski ID-jevi	OMIM: 606090 MGI: 1921364 HomoloGene: 10899 GeneCards: CROT
Lokacija gena (čovjek)
Hrom.	Hromosom 7 (čovjek)
Kraj	87,399,794 bp
Lokacija gena (miš)
Hrom.	Hromosom 5 (miš)
Kraj	9,047,324 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• aktivnost sa transferazom; • signaling receptor binding; • acyltransferase activity; • carnitine O-octanoyltransferase activity;
Ćelijska komponenta	• intracellular membrane-bounded organelle; • Peroksisom; • peroxisomal matrix; • citosol;
Biološki proces	• medium-chain fatty acid metabolic process; • coenzyme A metabolic process; • lipid metabolism; • fatty acid transport; • generation of precursor metabolites and energy; • fatty acid metabolic process; • fatty acid beta-oxidation using acyl-CoA oxidase; • fatty acid beta-oxidation; • carnitine metabolic process; • protein targeting to peroxisome;
	Izvori:Amigo / QuickGO
Ortolozi
	54677
	74114
	ENSG00000005469
	ENSMUSG00000003623
	Q9UKG9
	Q9DC50
	NM_001143935; NM_001243745; NM_021151
	NM_023733
	NP_001137407; NP_001230674; NP_066974
	NP_076222
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Peroksisomna karnitinska O-oktanoiltransferaza je enzim koji je kod ljudi kodiran genom CROT.^[5]^[6]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 612 aminokiselina, а molekulska težina 70.178 Da.^[7]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MENQLAKSTE	ERTFQYQDSL	PSLPVPSLEE	SLKKYLESVK	PFANQEEYKK
TEEIVQKFQS	GIGEKLHQKL	LERAKGKRNW	LEEWWLNVAY	LDVRIPSQLN
VNFAGPAAHF	EHYWPPKEGT	QLERGSITLW	HNLNYWQLLR	KEKVPVHKVG
NTPLDMNQFR	MLFSTCKVPG	ITRDSIMNYF	RTESEGRSPN	HIVVLCRGRA
FVFDVIHEGC	LVTPPELLRQ	LTYIHKKCHS	EPDGPGIAAL	TSEERTRWAK
AREYLIGLDP	ENLALLEKIQ	SSLLVYSMED	SSPHVTPEDY	SEIIAAILIG
DPTVRWGDKS	YNLISFSNGV	FGCNCDHAPF	DAMIMVNISY	YVDEKIFQNE
GRWKGSEKVR	DIPLPEELIF	IVDEKVLNDI	NQAKAQYLRE	ASDLQIAAYA
FTSFGKKLTK	NKMLHPDTFI	QLALQLAYYR	LHGHPGCCYE	TAMTRHFYHG
RTETMRSCTV	EAVRWCQSMQ	DPSVNLRERQ	QKMLQAFAKH	NKMMKDCSAG
KGFDRHLLGL	LLIAKEEGLP	VPELFTDPLF	SKSGGGGNFV	LSTSLVGYLR
VQGVVVPMVH	NGYGFFYHIR	DDRFVVACSA	WKSCPETDAE	KLVQLTFCAF
HDMIQLMNST	HL

Funkcija[uredi | uredi izvor]

Karnitin-oktanoiltransferaza (EC 2.3.1.137) je karnitin-aciltransferaza koja katalizira reverzibilni transfer masnih acil grupa između CoA i karnitina. Ovaj enzim regulira razgradnju masnih kiselina vrlo dugog lanca (VLCFA) izvođenjem ključnog koraka u transportu acilnih lanaca srednje dužine iz sisarskih peroksisoma u citosol i mitohondrije za daljnji raspad. Van der Leij et al (2000) pregledali su funkciju, strukturne karakteristike i filogenetiku gena ljudske karnitin-aciltransferaze, uključujući CROT (prema OMIM^[6])

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000005469 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000003623 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Ferdinandusse S, Mulders J, IJlst L, Denis S, Dacremont G, Waterham HR, Wanders RJ (Oct 1999). "Molecular cloning and expression of human carnitine octanoyltransferase: evidence for its role in the peroxisomal beta-oxidation of branched-chain fatty acids". Biochem Biophys Res Commun. 263 (1): 213–8. doi:10.1006/bbrc.1999.1340. PMID 10486279.
^ ^a ^b "Entrez Gene: CROT carnitine O-octanoyltransferase".
^ "UniProt, Q9UKG9" (jezik: engleski). Pristupljeno 24. 9. 2021.

Dopunska literatura[uredi | uredi izvor]

van der Leij FR, Huijkman NC, Boomsma C, et al. (2000). "Genomics of the human carnitine acyltransferase genes". Mol. Genet. Metab. 71 (1–2): 139–53. doi:10.1006/mgme.2000.3055. PMID 11001805.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Scherer SW, Cheung J, MacDonald JR, et al. (2003). "Human chromosome 7: DNA sequence and biology". Science. 300 (5620): 767–72. Bibcode:2003Sci...300..767S. doi:10.1126/science.1083423. PMC 2882961. PMID 12690205.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Morillas M, Gómez-Puertas P, Rubí B, et al. (2002). "Structural model of a malonyl-CoA-binding site of carnitine octanoyltransferase and carnitine palmitoyltransferase I: mutational analysis of a malonyl-CoA affinity domain". J. Biol. Chem. 277 (13): 11473–80. doi:10.1074/jbc.M111628200. PMID 11790793.
Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.

Vanjski linkovi[uredi | uredi izvor]

Lokacija ljudskog genoma CROT i stranica sa detaljima o genu CROT u UCSC Genome Browseru.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000005469 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000003623 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10486279-5] Ferdinandusse S, Mulders J, IJlst L, Denis S, Dacremont G, Waterham HR, Wanders RJ (Oct 1999). "Molecular cloning and expression of human carnitine octanoyltransferase: evidence for its role in the peroxisomal beta-oxidation of branched-chain fatty acids". Biochem Biophys Res Commun. 263 (1): 213–8. doi:10.1006/bbrc.1999.1340. PMID 10486279.

[entrez-6] "Entrez Gene: CROT carnitine O-octanoyltransferase".

[UniProt-7] "UniProt, Q9UKG9" (jezik: engleski). Pristupljeno 24. 9. 2021.

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p r u Enzimi
Teme	Aktivno mjesto Alosterna regulacija Difuzijski limitirani enzim EC broj Enzimska kataliza Inhibicija enzimskih reakcija Kinetika enzima Koenzim Kooperativnost Lineweaver–Burkov dijagram Michaelis–Mentenova kinetika Mjesto vezanja Spisak enzima
Tipovi	EC1 Oksidoreduktaze/spisak EC2 Transferaze/spisak EC3 Hidrolaze/spisak EC4 Lijaze/spisak EC5 Izomeraze/spisak EC6 Ligaze/spisak