PKIA

PKIA
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	1CMK, 1JLU, 1Q8T, 1XH4, 1XH5, 1XH6, 1XH7, 1XH8, 1XH9, 1XHA, 1YDR, 2C1A, 2C1B, 2F7E, 2GNI, 2JDS, 2JDT, 2JDV, 2L1L, 2UVY, 2UVZ, 2UW0, 2UW3, 2UW4, 2UW5, 2UW6, 2UW7, 2UW8, 2VNW, 2VNY, 2VO0, 2VO3, 2VO6, 2VO7, 3AMA, 3AMB, 3L9L, 3L9M, 3L9N, 3MVJ, 3NX8, 3OOG, 3OVV, 3OWP, 3OXT, 3P0M, 3POO, 3VQH, 3WYG, 4AXA, 4IAC, 4IAD, 4IAF, 4IAI, 4IAK, 4IAY, 4IAZ, 4IB0, 4IB1, 4IB3, 4IE9, 4IJ9, 4O21, 4O22, 4WB5, 4WB6, 4WB7, 4WB8, 4Z83, 4Z84, 5DH9, 1Q61, 3X2U, 3X2W, 1Q62, 3X2V, 4UJA, 4UJ9, 4UJB, 4UJ2, 4UJ1
Identifikatori
Aliasi	PKIA
Vanjski ID-jevi	OMIM: 606059 MGI: 104747 HomoloGene: 7473 GeneCards: PKIA
Lokacija gena (čovjek)
Hrom.	Hromosom 8 (čovjek)
Kraj	78,605,267 bp
Lokacija gena (miš)
Hrom.	Hromosom 3 (miš)
Kraj	7,510,426 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• protein kinase inhibitor activity; • GO:0001948, GO:0016582 vezivanje za proteine; • cAMP-dependent protein kinase inhibitor activity; • protein kinase A catalytic subunit binding; • kinase activity;
Ćelijska komponenta	• citoplazma; • jedro;
Biološki proces	• regulation of G2/M transition of mitotic cell cycle; • GO:0048553 negative regulation of catalytic activity; • GO:1901227 negative regulation of transcription by RNA polymerase II; • negative regulation of cAMP-dependent protein kinase activity; • negative regulation of protein kinase activity; • negative regulation of protein import into nucleus; • negative regulation of protein serine/threonine kinase activity; • Fosforilacija;
	Izvori:Amigo / QuickGO
Ortolozi
	5569
	18767
	ENSG00000171033
	ENSMUSG00000027499
	P61925
	P63248
	NM_181839; NM_006823
	NM_008862
	NP_006814; NP_862822; NP_006814.1; NP_862822.1
	NP_032888
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

cAMP-ovisni inhibitor alfa protein-kinaze je protein koji je kod ljudi kodiran genom PKIA.^[5]^[6]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 76 aminokiselina, а molekulska težina 7.989 Da.^[7]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10		20		30		40		50
MTDVETTYAD		FIASGRTGRR		NAIHDILVSS		ASGNSNELAL		KLAGLDINKT
EGEEDAQRSS		TEQSGEAQGE		AAKSES

Funkcija[uredi | uredi izvor]

Protein kodiran ovim genom član je porodice inhibitora protein kinaze (PKA) ovisne o cAMP-u. Pokazalo se da ovaj protein stupa u interakciju i inhibira aktivnosti katalitskih podjedinica C alfa i C beta PKA. Prijavljene su Alternativno prerađene varijante transkripta, koje kodiraju isti protein.^[6]

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000171033 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000027499 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Olsen SR, Uhler MD (Feb 1992). "Inhibition of protein kinase-A by overexpression of the cloned human protein kinase inhibitor". Mol Endocrinol. 5 (9): 1246–1256. doi:10.1210/mend-5-9-1246. PMID 1770951.
^ ^a ^b "Entrez Gene: PKIA protein kinase (cAMP-dependent, catalytic) inhibitor alpha".
^ "UniProt, P61925" (jezik: engleski). Pristupljeno 20. 9. 2021.

Dopunska literatura[uredi | uredi izvor]

Olsen SR, Uhler MD (1991). "Isolation and characterization of cDNA clones for an inhibitor protein of cAMP-dependent protein kinase". J. Biol. Chem. 266 (17): 11158–11162. doi:10.1016/S0021-9258(18)99142-8. PMID 1710219.
Knighton DR, Zheng JH, Ten Eyck LF, et al. (1991). "Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase". Science. 253 (5018): 414–420. doi:10.1126/science.1862343. PMID 1862343.
Van Patten SM, Heisermann GJ, Cheng HC, Walsh DA (1987). "Tyrosine kinase catalyzed phosphorylation and inactivation of the inhibitor protein of the cAMP-dependent protein kinase". J. Biol. Chem. 262 (7): 3398–3403. doi:10.1016/S0021-9258(18)61516-9. PMID 2434500.
Scott JD, Fischer EH, Demaille JG, Krebs EG (1985). "Identification of an inhibitory region of the heat-stable protein inhibitor of the cAMP-dependent protein kinase". Proc. Natl. Acad. Sci. U.S.A. 82 (13): 4379–4383. doi:10.1073/pnas.82.13.4379. PMC 390417. PMID 2989819.
Scott JD, Glaccum MB, Fischer EH, Krebs EG (1986). "Primary-structure requirements for inhibition by the heat-stable inhibitor of the cAMP-dependent protein kinase". Proc. Natl. Acad. Sci. U.S.A. 83 (6): 1613–1616. doi:10.1073/pnas.83.6.1613. PMC 323133. PMID 3456605.
Wen W, Taylor SS, Meinkoth JL (1995). "The expression and intracellular distribution of the heat-stable protein kinase inhibitor is cell cycle regulated". J. Biol. Chem. 270 (5): 2041–2046. doi:10.1074/jbc.270.5.2041. PMID 7836431.
Baude EJ, Dignam SS, Olsen SR, et al. (1994). "Glutamic acid 203 of the cAMP-dependent protein kinase catalytic subunit participates in the inhibition by two isoforms of the protein kinase inhibitor". J. Biol. Chem. 269 (3): 2316–2323. doi:10.1016/S0021-9258(17)42170-3. PMID 7905001.
Baude EJ, Dignam SS, Reimann EM, Uhler MD (1994). "Evidence for the importance of hydrophobic residues in the interactions between the cAMP-dependent protein kinase catalytic subunit and the protein kinase inhibitors". J. Biol. Chem. 269 (27): 18128–18133. doi:10.1016/S0021-9258(17)32426-2. PMID 8027074.
Fritz CC, Green MR (1997). "HIV Rev uses a conserved cellular protein export pathway for the nucleocytoplasmic transport of viral RNAs". Curr. Biol. 6 (7): 848–854. doi:10.1016/S0960-9822(02)00608-5. PMID 8805303. S2CID 16705807.
Zimmermann B, Chiorini JA, Ma Y, et al. (1999). "PrKX is a novel catalytic subunit of the cAMP-dependent protein kinase regulated by the regulatory subunit type I". J. Biol. Chem. 274 (9): 5370–5378. doi:10.1074/jbc.274.9.5370. PMID 10026146.
Yu J, Yu L, Chen Z, et al. (2002). "Protein inhibitor of neuronal nitric oxide synthase interacts with protein kinase A inhibitors". Brain Res. Mol. Brain Res. 99 (2): 145–149. doi:10.1016/S0169-328X(02)00104-3. PMID 11978406.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–16903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Gassel M, Breitenlechner CB, Rüger P, et al. (2003). "Mutants of protein kinase A that mimic the ATP-binding site of protein kinase B (AKT)". J. Mol. Biol. 329 (5): 1021–1034. doi:10.1016/S0022-2836(03)00518-7. PMID 12798691.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–2127. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000171033 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000027499 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid1770951-5] Olsen SR, Uhler MD (Feb 1992). "Inhibition of protein kinase-A by overexpression of the cloned human protein kinase inhibitor". Mol Endocrinol. 5 (9): 1246–1256. doi:10.1210/mend-5-9-1246. PMID 1770951.

[entrez-6] "Entrez Gene: PKIA protein kinase (cAMP-dependent, catalytic) inhibitor alpha".

[UniProt-7] "UniProt, P61925" (jezik: engleski). Pristupljeno 20. 9. 2021.

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