POLR3F
Podjedinica RPC6 DNK-usmjerene RNK-polimeraze III je enzim koji je kod ljudi kodiran genom POLR3F.[5][6]
Funkcija[uredi | uredi izvor]
Protein koji je kodiran ovim genom jedna je od više od desetak podjedinica koje tvore eukariotsku RNK polimerazu III (RNK Pol III), koja transkribira 5S gene ribosomske i transportne RNK. Pokazalo se da ovaj protein veže i za TFIIIB90 i za TBP, dvije podjedinice RNK-polimeraze III, faktor inicijacije transkripcije IIIB (TFIIIB). Za razliku od većine ostalih RNK Pol III podjedinica, kodirani protein je jedinstven za ovu polimerazu.[6]
Modelni organizni[uredi | uredi izvor]
U proučavanju funkcije POLR3F korišteni su i Model organizmi. Uvjetna linija nokaut miša, zvana Polr3ftm1a (EUCOMM) Wtsi generirana je u Wellcome Trust Sanger Institute.[7] Muške i ženske životinje prolazile su standardizirani fenotipski skrining[8] to determine the effects of deletion.[9][10][11][12] Obavljeni su i dodatni pregledi: Dubinsko imunsko fenotipiziranje[13]
| Svojstvo | Fenotip |
|---|---|
| Svi podaci raspoloživi su na linku.[8][13] | |
| Insulin | Normalan |
| Vijabilnost homozigota na P14 | Nenormalna |
| Studija letalne recesivnosti | Nenormalna |
| Tjelesna težina | Normalna |
| Neurološka procjena | Normalna |
| Snaga stisakanja | Normalna |
| Dismorfologija | Normalna |
| Indirektna kalorimetrija | Normalna |
| Test tolerancije glukoze | Normalan |
| Slušni odgovor moždanog debla | Normaln |
| DEXA | Normalna |
| Radiografija | Normalna |
| Morfologija oka | Normalna |
| Klinička hemija | Normalna |
| Hematologija 16 sedmica | Normalna |
| Leukociti periferne krvi 16 sedmica | Normalni |
| Micronukleus test | Nenormalan |
| Salmonella infekcija | Normalna |
| Epidermni imunski sastav | Normalan |
| Izazov gripe | Normalan |
Reference[uredi | uredi izvor]
- ^ a b c GRCh38: Ensembl release 89: ENSG00000132664 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000027427 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Wang Z, Roeder RG (maj 1997). "Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation". Genes & Development. 11 (10): 1315–26. doi:10.1101/gad.11.10.1315. PMID 9171375.
- ^ a b "Entrez Gene: POLR3F polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa".
- ^ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
- ^ a b "International Mouse Phenotyping Consortium".
- ^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- ^ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ^ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
- ^ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
- ^ a b "Infection and Immunity Immunophenotyping (3i) Consortium". Arhivirano s originala, 21. 5. 2015. Pristupljeno 9. 5. 2021.
Dopunska literatura[uredi | uredi izvor]
- Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (Sep 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
- Hu P, Wu S, Sun Y, Yuan CC, Kobayashi R, Myers MP, Hernandez N (Nov 2002). "Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits" (PDF). Molecular and Cellular Biology. 22 (22): 8044–55. doi:10.1128/MCB.22.22.8044-8055.2002. PMC 134740. PMID 12391170. Arhivirano s originala (PDF), 22. 9. 2017. Pristupljeno 9. 5. 2021.
- Jones E, Kimura H, Vigneron M, Wang Z, Roeder RG, Cook PR (Jan 2000). "Isolation and characterization of monoclonal antibodies directed against subunits of human RNA polymerases I, II, and III". Experimental Cell Research. 254 (1): 163–72. doi:10.1006/excr.1999.4739. PMID 10623476.
- Hsieh YJ, Kundu TK, Wang Z, Kovelman R, Roeder RG (Nov 1999). "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity". Molecular and Cellular Biology. 19 (11): 7697–704. doi:10.1128/mcb.19.11.7697. PMC 84812. PMID 10523658.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Jang KL, Collins MK, Latchman DS (1992). "The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC". Journal of Acquired Immune Deficiency Syndromes. 5 (11): 1142–7. PMID 1403646.