POLR3F

POLR3F
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2DK5, 2YU3
Identifikatori
Aliasi	POLR3F
Vanjski ID-jevi	OMIM: 617455 MGI: 1924086 HomoloGene: 4722 GeneCards: POLR3F
Lokacija gena (čovjek)
Hrom.	Hromosom 20 (čovjek)
Kraj	18,484,646 bp
Lokacija gena (miš)
Hrom.	Hromosom 2 (miš)
Kraj	144,383,915 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• DNA-directed 5'-3' RNA polymerase activity; • GO:0001948, GO:0016582 vezivanje za proteine; • RNA polymerase III activity; • double-stranded DNA binding; • vezivanje sa DNK;
Ćelijska komponenta	• RNA polymerase III complex; • citosol; • jedro; • nukleoplazma;
Biološki proces	• positive regulation of interferon-beta production; • immune system process; • defense response to virus; • positive regulation of innate immune response; • transcription by RNA polymerase III; • Urođeni imunski sistem; • regulation of transcription by RNA polymerase III; • positive regulation of type I interferon production;
	Izvori:Amigo / QuickGO
Ortolozi
	10621
	70408
	ENSG00000132664
	ENSMUSG00000027427
	Q9H1D9
	Q921X6
	NM_001282526; NM_006466
	NM_029763; NM_027417
	NP_001269455; NP_006457
	NP_084039
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Podjedinica RPC6 DNK-usmjerene RNK-polimeraze III je enzim koji je kod ljudi kodiran genom POLR3F.^[5]^[6]

Funkcija

Protein koji je kodiran ovim genom jedna je od više od desetak podjedinica koje tvore eukariotsku RNK polimerazu III (RNK Pol III), koja transkribira 5S gene ribosomske i transportne RNK. Pokazalo se da ovaj protein veže i za TFIIIB90 i za TBP, dvije podjedinice RNK-polimeraze III, faktor inicijacije transkripcije IIIB (TFIIIB). Za razliku od većine ostalih RNK Pol III podjedinica, kodirani protein je jedinstven za ovu polimerazu.^[6]

Modelni organizni

U proučavanju funkcije POLR3F korišteni su i Model organizmi. Uvjetna linija nokaut miša, zvana Polr3f^{tm1a (EUCOMM) Wtsi} generirana je u Wellcome Trust Sanger Institute.^[7] Muške i ženske životinje prolazile su standardizirani fenotipski skrining^[8] to determine the effects of deletion.^[9]^[10]^[11]^[12] Obavljeni su i dodatni pregledi: Dubinsko imunsko fenotipiziranje^[13]

Fenotip *Polr3f* nokaut-miša
Svojstvo	Fenotip
Svi podaci raspoloživi su na linku.^[8]^[13]
Insulin	Normalan
Vijabilnost homozigota na P14	Nenormalna
Studija letalne recesivnosti	Nenormalna
Tjelesna težina	Normalna
Neurološka procjena	Normalna
Snaga stisakanja	Normalna
Dismorfologija	Normalna
Indirektna kalorimetrija	Normalna
Test tolerancije glukoze	Normalan
Slušni odgovor moždanog debla	Normaln
DEXA	Normalna
Radiografija	Normalna
Morfologija oka	Normalna
Klinička hemija	Normalna
Hematologija 16 sedmica	Normalna
Leukociti periferne krvi 16 sedmica	Normalni
Micronukleus test	Nenormalan
Salmonella infekcija	Normalna
Epidermni imunski sastav	Normalan
Izazov gripe	Normalan

Reference

1 2 3 GRCh38: Ensembl release 89: ENSG00000132664 - Ensembl, maj 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000027427 - Ensembl, maj 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Wang Z, Roeder RG (maj 1997). "Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation". Genes & Development. 11 (10): 1315–26. doi:10.1101/gad.11.10.1315. PMID 9171375.
1 2 "Entrez Gene: POLR3F polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa".
↑ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
1 2 "International Mouse Phenotyping Consortium".
↑ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
↑ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
↑ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
↑ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
1 2 "Infection and Immunity Immunophenotyping (3i) Consortium". Arhivirano s originala, 21. 5. 2015. Pristupljeno 9. 5. 2021.

Dopunska literatura

Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (Sep 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
Hu P, Wu S, Sun Y, Yuan CC, Kobayashi R, Myers MP, Hernandez N (Nov 2002). "Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits" (PDF). Molecular and Cellular Biology. 22 (22): 8044–55. doi:10.1128/MCB.22.22.8044-8055.2002. PMC 134740. PMID 12391170. Arhivirano s originala (PDF), 22. 9. 2017. Pristupljeno 9. 5. 2021.
Jones E, Kimura H, Vigneron M, Wang Z, Roeder RG, Cook PR (Jan 2000). "Isolation and characterization of monoclonal antibodies directed against subunits of human RNA polymerases I, II, and III". Experimental Cell Research. 254 (1): 163–72. doi:10.1006/excr.1999.4739. PMID 10623476.
Hsieh YJ, Kundu TK, Wang Z, Kovelman R, Roeder RG (Nov 1999). "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity". Molecular and Cellular Biology. 19 (11): 7697–704. doi:10.1128/mcb.19.11.7697. PMC 84812. PMID 10523658.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Jang KL, Collins MK, Latchman DS (1992). "The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC". Journal of Acquired Immune Deficiency Syndromes. 5 (11): 1142–7. PMID 1403646.

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000132664 - Ensembl, maj 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000027427 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9171375-5] Wang Z, Roeder RG (maj 1997). "Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation". Genes & Development. 11 (10): 1315–26. doi:10.1101/gad.11.10.1315. PMID 9171375.

[entrez-6] 1 2 "Entrez Gene: POLR3F polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa".

[mgp_reference-7] Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.

[IMPCsearch_ref-8] 1 2 "International Mouse Phenotyping Consortium".

[pmid21677750-9] Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.

[mouse_library-10] Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.

[mouse_for_all_reasons-11] Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.

[pmid23870131-12] White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.

[iii_ref-13] 1 2 "Infection and Immunity Immunophenotyping (3i) Consortium". Arhivirano s originala, 21. 5. 2015. Pristupljeno 9. 5. 2021.

[1]

[2]

[3]

[4]

[5]

[6]

[7]

[8]

[9]

[10]

[11]

[12]

[13]