POLR3G
Polimerazno (RNK) III (DNK-usmjereni) polipeptid G (32kD) jest protein koji je kod ljudi kodiran genom POLR3G sa hromosoma 5.[5]
Aminokiselinska sekvenca[uredi | uredi izvor]
Dužina polipeptidnog lanca je 223 aminokiselina, а molekulska težina 25.914 Da.[6]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAGNKGRGRA | AYTFNIEAVG | FSKGEKLPDV | VLKPPPLFPD | TDYKPVPLKT | ||||
| GEGEEYMLAL | KQELRETMKR | MPYFIETPEE | RQDIERYSKR | YMKVYKEEWI | ||||
| PDWRRLPREM | MPRNKCKKAG | PKPKKAKDAG | KGTPLTNTED | VLKKMEELEK | ||||
| RGDGEKSDEE | NEEKEGSKEK | SKEGDDDDDD | DAAEQEEYDE | EEQEEENDYI | ||||
| NSYFEDGDDF | GADSDDNMDE | ATY |
Modenil organizmi[uredi | uredi izvor]
U proučavanju funkcije POLR3G korišteni su modelni organizmi. Uvjetna nokaut-mišja linija pod nazivom Polr3gtm1a (EUCOMM) Wtsi generirana je na Institutu Wellcome Trust Sanger.[7] Mužjaci i ženke podvrgnuti su standardiziranom fenotipskom pregledu[8] to determine the effects of deletion.[9][10][11][12] Dodatni pregledi obavljeni su dubinskom imunološkom fenotipizacijom.[13] Od 23 analizirana pokazatelja, ozbiljnije nenormalnosti nađene su u samo dva slučaja.
| Svojstvo | Fenotip |
|---|---|
| Svi podaci raspoloživi na linku:[8][13] | |
| Insulin | Normalan |
| Vijabilnost homozigota na P14 | Nenormalan |
| Studija recesivne letalnosti | Nenormalan |
| Tjelesna težina | Normalan |
| Neurološka procjena | Normalan |
| Jačina stiska | Normalan |
| Dismorfologija | Normalan |
| Indirektna kalorimetrija | Normalan |
| Test tolerancije glukoze | Normalan |
| Slušni odgovor moždanog stabla | Normalan |
| DEXA | Normalan |
| Radiografija | Normalan |
| Morfologija oka | Normalan |
| Klinička hemija | Normalan |
| Hematologija 16 sedmica | Normalan |
| Leukociti periferne krvi 16 sedmica | Normalan |
| Težina srca | Normalan |
| Funkcija citotoksičnih T-ćelija | Normalan |
| Imunofenotipizacija slezene | Normalan |
| Imunofenotipizacija mezenternog limfnog čvora | Normalan |
| Imunofenotipizacija koštane srži | Normalan |
| Sastav epidermne imunosti | Normalan |
| Izazov gripe | Normalan |
Reference[uredi | uredi izvor]
- ^ a b c GRCh38: Ensembl release 89: ENSG00000113356 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000035834 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Entrez Gene: Polymerase (RNA) III (DNA directed) polypeptide G (32kD)". Pristupljeno 1. 4. 2014.
- ^ "UniProt, O15318" (jezik: engleski). Pristupljeno 19. 10. 2021.
- ^ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
- ^ a b "International Mouse Phenotyping Consortium".
- ^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- ^ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ^ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
- ^ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Sanger Institute Mouse Genetics Project, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
- ^ a b "Infection and Immunity Immunophenotyping (3i) Consortium". Arhivirano s originala, 21. 5. 2015. Pristupljeno 19. 10. 2021.
Dopunska literatura[uredi | uredi izvor]
- Ablasser, A; Bauernfeind, F; Hartmann, G; Latz, E; Fitzgerald, K. A.; Hornung, V (2009). "RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate" (PDF). Nature Immunology. 10 (10): 1065–72. doi:10.1038/ni.1779. PMC 3878616. PMID 19609254.
- Chiu, Y. H.; MacMillan, J. B.; Chen, Z. J. (2009). "RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway". Cell. 138 (3): 576–91. doi:10.1016/j.cell.2009.06.015. PMC 2747301. PMID 19631370.
- Chantarangsu, S; Mushiroda, T; Mahasirimongkol, S; Kiertiburanakul, S; Sungkanuparph, S; Manosuthi, W; Tantisiriwat, W; Charoenyingwattana, A; Sura, T; Takahashi, A; Kubo, M; Kamatani, N; Chantratita, W; Nakamura, Y (2011). "Genome-wide association study identifies variations in 6p21.3 associated with nevirapine-induced rash". Clinical Infectious Diseases. 53 (4): 341–8. doi:10.1093/cid/cir403. PMID 21810746.
- Wong, R. C.; Pollan, S; Fong, H; Ibrahim, A; Smith, E. L.; Ho, M; Laslett, A. L.; Donovan, P. J. (2011). "A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells". Stem Cells. 29 (10): 1517–27. doi:10.1002/stem.714. PMID 21898682. S2CID 24305950.
- Jang, K. L.; Collins, M. K.; Latchman, D. S. (1992). "The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC". Journal of Acquired Immune Deficiency Syndromes. 5 (11): 1142–7. PMID 1403646.
- Wang, Z; Roeder, R. G. (1997). "Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation". Genes & Development. 11 (10): 1315–26. doi:10.1101/gad.11.10.1315. PMID 9171375.
- Hu, P; Wu, S; Sun, Y; Yuan, C. C.; Kobayashi, R; Myers, M. P.; Hernandez, N (2002). "Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits". Molecular and Cellular Biology. 22 (22): 8044–55. doi:10.1128/MCB.22.22.8044-8055.2002. PMC 134740. PMID 12391170.