SCAMP2

SCAMP2
Identifikatori
Aliasi	SCAMP2
Vanjski ID-jevi	OMIM: 606912 MGI: 1346518 HomoloGene: 4163 GeneCards: SCAMP2
Lokacija gena (čovjek)
Hrom.	Hromosom 15 (čovjek)
Kraj	74,873,365 bp
Lokacija gena (miš)
Hrom.	Hromosom 9 (miš)
Kraj	57,496,078 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• GO:0001948, GO:0016582 vezivanje za proteine;
Ćelijska komponenta	• integral component of membrane; • endozom; • recycling endosome membrane; • Golđijev aparat; • trans-Golgi network membrane; • Egzosom; • transport vesicle; • intracellular membrane-bounded organelle; • membrana; • Golđijeva membrana;
Biološki proces	• protein transport; • post-Golgi vesicle-mediated transport; • GO:0015915 transport;
	Izvori:Amigo / QuickGO
Ortolozi
	10066
	24044
	ENSG00000140497
	ENSMUSG00000040188
	O15127
	Q9ERN0
	NM_005697; NM_001320778
	NM_022813; NM_001378928
	NP_001307707; NP_005688
	NP_073724; NP_001365857
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Sekretorni membranski protein povezan sa nosačem 2 je protein koji je kod ljudi kodiran genom SCAMP2.^[5]^[6] Međunarodni konzorcij za radijacijsku hibridizacijumapirao je gen SCAMP2 na hromosomu 15 (sts-W74766).

Ovaj genski proizvod pripada porodici proteina SCAMP, koji su sekretorni nosači, membranski proteini. Oni funkcioniraju kao nosači površine ćelije u putevima recikliranja nakon Golgijevog aparata. Različiti članovi porodice su srodni proizvodi različitih gena i obično su eksprimirani zajedno. Ova otkrića sugeriraju da SCAMP-ovi mogu funkcionirati na istom mjestu tokom transporta vezikula, umjesto na odvojenim putovima.^[6]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 329 aminokiselina, a molekulska težina 36.649 Da.^[7].

10	20	30	40	50
MSAFDTNPFA	DPVDVNPFQD	PSVTQLTNAP	QGGLAEFNPF	SETNAATTVP
VTQLPGSSQP	AVLQPSVEPT	QPTPQAVVSA	AQAGLLRQQE	ELDRKAAELE
RKERELQNTV	ANLHVRQNNW	PPLPSWCPVK	PCFYQDFSTE	IPADYQRICK
MLYYLWMLHS	VTLFLNLLAC	LAWFSGNSSK	GVDFGLSILW	FLIFTPCAFL
CWYRPIYKAF	RSDNSFSFFV	FFFVFFCQIG	IYIIQLVGIP	GLGDSGWIAA
LSTLDNHSLA	ISVIMMVVAG	FFTLCAVLSV	FLLQRVHSLY	RRTGASFQQA
QEEFSQGIFS	SRTFHRAASS	AAQGAFQGN

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija[uredi | uredi izvor]

Singleton et al. (1997) pojačali su SCAMP2 pomoću PCR-a, koristeći prajmere dizajnirane od SCAMP1. Klonirali su cDNK pune dužine iz cDNK biblioteke HeLa ćelijeđa. Također su identificirali SCAMP3 kao još jedan paralog u ćelijskoj biblioteci HeLa. SCAMP2 kodira izvedeni protein od 329 aminokiselina s izračunatom molekulskom masom od 36,6 kD. Protein dijeli strukturne karakteristike sa SCAMP1 i SCAMP3, uključujući segment sličan leucinskom zatvaraču, element bogat prolinom, prošireno središnje jezgro koju uključuječetiri navodna transmembranska domena, polarni segment i C-kraj bogat alaninom. SCAMP2 dijeli 54% i 56% ukupnog identiteta sekvence sa SCAMP1, odnosno SCAMP3, odnosno 94% identiteta s mišjim homologom. Najrazličitija područja su u N-kraji. Northern blot analizom otkriven je transkript SCAMP2 od 1,3 kb, s najvećom ekspresijom u srcu, posteljici i skeletnim mišićima, srednjom ekspresijom u plućima, gušterači i jetri i niskom ekspresijom u mozgu i bubrezima. Imunofluorescentna lokalizacija u HeLa ćelijama pokazala je tačkasta obojenja sa određenim perinuklearnim i perifernim obogaćivanjem, te djelimičnu kolokalizaciju sa SCAMP1 i SCAMP3.^[8]

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000140497 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000040188 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Singleton DR, Wu TT, Castle JD (Nov 1997). "Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions". J Cell Sci. 110 (17): 2099–107. PMID 9378760.
^ ^a ^b "Entrez Gene: SCAMP2 secretory carrier membrane protein 2".
^ "UniProt, O15127". Pristupljeno 18. 7. 2021.
^ Singleton, D. R., Wu, T. T., Castle, J. D. Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions. J. Cell Sci. 110: 2099-2107, 1997. PubMed: 9378760

Dopunska literatura[uredi | uredi izvor]

Wu TT, Castle JD (1997). "Evidence for colocalization and interaction between 37 and 39 kDa isoforms of secretory carrier membrane proteins (SCAMPs)". J. Cell Sci. 110 (13): 1533–41. PMID 9224770.
Guo Z, Liu L, Cafiso D, Castle D (2002). "Perturbation of a very late step of regulated exocytosis by a secretory carrier membrane protein (SCAMP2)-derived peptide". J. Biol. Chem. 277 (38): 35357–63. doi:10.1074/jbc.M202259200. PMID 12124380.
Liu L, Guo Z, Tieu Q, et al. (2003). "Role of Secretory Carrier Membrane Protein SCAMP2 in Granule Exocytosis". Mol. Biol. Cell. 13 (12): 4266–78. doi:10.1091/mbc.E02-03-0136. PMC 138632. PMID 12475951.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Lin PJ, Williams WP, Luu Y, et al. (2005). "Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7". J. Cell Sci. 118 (Pt 9): 1885–97. doi:10.1242/jcs.02315. PMID 15840657.
Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Müller HK, Wiborg O, Haase J (2006). "Subcellular redistribution of the serotonin transporter by secretory carrier membrane protein 2". J. Biol. Chem. 281 (39): 28901–9. doi:10.1074/jbc.M602848200. PMID 16870614.
Liao H, Ellena J, Liu L, et al. (2007). "Secretory carrier membrane protein SCAMP2 and phosphatidylinositol 4,5-bisphosphate interactions in the regulation of dense core vesicle exocytosis". Biochemistry. 46 (38): 10909–20. doi:10.1021/bi701121j. PMID 17713930.

Vanjski linkovi[uredi | uredi izvor]

SCAMP2 lokacija ljudskog genoma UCSC Genome Browser.

SCAMP2 detalji ljudskog genoma u UCSC Genome Browser.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000140497 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000040188 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9378760-5] Singleton DR, Wu TT, Castle JD (Nov 1997). "Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions". J Cell Sci. 110 (17): 2099–107. PMID 9378760.

[entrez-6] "Entrez Gene: SCAMP2 secretory carrier membrane protein 2".

[UniProt-7] "UniProt, O15127". Pristupljeno 18. 7. 2021.

[8] Singleton, D. R., Wu, T. T., Castle, J. D. Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions. J. Cell Sci. 110: 2099-2107, 1997. PubMed: 9378760

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