SENP6
Sentrin-specifična proteaza 6 jest enzim koji je kod ljudi kodiran genom SENP6.[5]
Aminokiselinska sekvenca[uredi | uredi izvor]
Dužina polipeptidnog lanca je 1.112 aminokiselina, а molekulska težina 126.146 Da.[6]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAAGKSGGSA | GEITFLEALA | RSESKRDGGF | KNNWSFDHEE | ESEGDTDKDG | ||||
| TNLLSVDEDE | DSETSKGKKL | NRRSEIVANS | SGEFILKTYV | RRNKSESFKT | ||||
| LKGNPIGLNM | LSNNKKLSEN | TQNTSLCSGT | VVHGRRFHHA | HAQIPVVKTA | ||||
| AQSSLDRKER | KEYPPHVQKV | EINPVRLSRL | QGVERIMKKT | EESESQVEPE | ||||
| IKRKVQQKRH | CSTYQPTPPL | SPASKKCLTH | LEDLQRNCRQ | AITLNESTGP | ||||
| LLRTSIHQNS | GGQKSQNTGL | TTKKFYGNNV | EKVPIDIIVN | CDDSKHTYLQ | ||||
| TNGKVILPGA | KIPKITNLKE | RKTSLSDLND | PIILSSDDDD | DNDRTNRRES | ||||
| ISPQPADSAC | SSPAPSTGKV | EAALNENTCR | AERELRSIPE | DSELNTVTLP | ||||
| RKARMKDQFG | NSIINTPLKR | RKVFSQEPPD | ALALSCQSSF | DSVILNCRSI | ||||
| RVGTLFRLLI | EPVIFCLDFI | KIQLDEPDHD | PVEIILNTSD | LTKCEWCNVR | ||||
| KLPVVFLQAI | PAVYQKLSIQ | LQMNKEDKVW | NDCKGVNKLT | NLEEQYIILI | ||||
| FQNGLDPPAN | MVFESIINEI | GIKNNISNFF | AKIPFEEANG | RLVACTRTYE | ||||
| ESIKGSCGQK | ENKIKTVSFE | SKIQLRSKQE | FQFFDEEEET | GENHTIFIGP | ||||
| VEKLIVYPPP | PAKGGISVTN | EDLHCLNEGE | FLNDVIIDFY | LKYLVLEKLK | ||||
| KEDADRIHIF | SSFFYKRLNQ | RERRNHETTN | LSIQQKRHGR | VKTWTRHVDI | ||||
| FEKDFIFVPL | NEAAHWFLAV | VCFPGLEKPK | YEPNPHYHEN | AVIQKCSTVE | ||||
| DSCISSSASE | MESCSQNSSA | KPVIKKMLNK | KHCIAVIDSN | PGQEESDPRY | ||||
| KRNICSVKYS | VKKINHTASE | NEEFNKGEST | SQKVADRTKS | ENGLQNESLS | ||||
| STHHTDGLSK | IRLNYSDESP | EAGKMLEDEL | VDFSEDQDNQ | DDSSDDGFLA | ||||
| DDNCSSEIGQ | WHLKPTICKQ | PCILLMDSLR | GPSRSNVVKI | LREYLEVEWE | ||||
| VKKGSKRSFS | KDVMKGSNPK | VPQQNNFSDC | GVYVLQYVES | FFENPILSFE | ||||
| LPMNLANWFP | PPRMRTKREE | IRNIILKLQE | DQSKEKRKHK | DTYSTEAPLG | ||||
| EGTEQYVNSI | SD |
Funkcija[uredi | uredi izvor]
Molekule slični ubikvitinu (UBL), kao što je mali modifikator povezan s ubikvitinom 1 (SUMO1), strukturno su povezani s ubikvitinom i mogu se vezati za ciljanje proteina na sličan način kao i ubikvitin. Međutim, kovalentno vezivanje UBL-a ne dovodi do razgradnje modificiranih proteina. Modifikacija SUMO1 je uključena u ciljanje RANGAP1 na kompleks iedarnih pora, kao i u stabilizaciju I-kapa-B-alfa (NFKBIA; MIM 164008) od proteasomske razgradnje 26S. Poput ubikvitina, UBL se sintetiziraju kao proteini prekursori, s jedan ili više aminokiselina nakon C-terminalnog glicin-glicinskih ostataka zrelog UBL proteina. Stoga, prije njihove konjugacije na ciljane proteine, repne sekvence UBL prekursora treba ukloniti UBL-specifičnim proteazama, kao što je SENP6.
Reference[uredi | uredi izvor]
- ^ a b c GRCh38: Ensembl release 89: ENSG00000112701 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000034252 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Entrez Gene: SENP6 SUMO1/sentrin specific peptidase 6".
- ^ "UniProt, Q9GZR1" (jezik: engleski). Pristupljeno 10. 10. 2021.
Dopunska literatura[uredi | uredi izvor]
- Mikolajczyk J, Drag M, Békés M, Cao JT, Ronai Z, Salvesen GS (septembar 2007). "Small ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs". The Journal of Biological Chemistry. 282 (36): 26217–24. doi:10.1074/jbc.M702444200. PMID 17591783.
- Drag M, Mikolajczyk J, Krishnakumar IM, Huang Z, Salvesen GS (januar 2008). "Activity profiling of human deSUMOylating enzymes (SENPs) with synthetic substrates suggests an unexpected specificity of two newly characterized members of the family". The Biochemical Journal. 409 (2): 461–9. doi:10.1042/BJ20070940. PMID 17916063. S2CID 12234952.
- Yeh ET, Gong L, Kamitani T (maj 2000). "Ubiquitin-like proteins: new wines in new bottles". Gene. 248 (1–2): 1–14. doi:10.1016/S0378-1119(00)00139-6. PMID 10806345.
- Nagase T, Ishikawa K, Suyama M, Kikuno R, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (oktobar 1998). "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 5 (5): 277–86. doi:10.1093/dnares/5.5.277. PMID 9872452.
- Kim KI, Baek SH, Jeon YJ, Nishimori S, Suzuki T, Uchida S, Shimbara N, Saitoh H, Tanaka K, Chung CH (maj 2000). "A new SUMO-1-specific protease, SUSP1, that is highly expressed in reproductive organs". The Journal of Biological Chemistry. 275 (19): 14102–6. doi:10.1074/jbc.275.19.14102. PMID 10799485.
- Goodson ML, Hong Y, Rogers R, Matunis MJ, Park-Sarge OK, Sarge KD (maj 2001). "Sumo-1 modification regulates the DNA binding activity of heat shock transcription factor 2, a promyelocytic leukemia nuclear body associated transcription factor". The Journal of Biological Chemistry. 276 (21): 18513–8. doi:10.1074/jbc.M008066200. PMID 11278381.
- Tojo M, Matsuzaki K, Minami T, Honda Y, Yasuda H, Chiba T, Saya H, Fujii-Kuriyama Y, Nakao M (novembar 2002). "The aryl hydrocarbon receptor nuclear transporter is modulated by the SUMO-1 conjugation system". The Journal of Biological Chemistry. 277 (48): 46576–85. doi:10.1074/jbc.M205987200. PMID 12354770.
- Bailey D, O'Hare P (januar 2004). "Characterization of the localization and proteolytic activity of the SUMO-specific protease, SENP1". The Journal of Biological Chemistry. 279 (1): 692–703. doi:10.1074/jbc.M306195200. PMID 14563852.
- Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (august 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
- Choi SJ, Chung SS, Rho EJ, Lee HW, Lee MH, Choi HS, Seol JH, Baek SH, Bang OS, Chung CH (oktobar 2006). "Negative modulation of RXRalpha transcriptional activity by small ubiquitin-related modifier (SUMO) modification and its reversal by SUMO-specific protease SUSP1". The Journal of Biological Chemistry. 281 (41): 30669–77. doi:10.1074/jbc.M604033200. PMID 16912044.
- Mukhopadhyay D, Ayaydin F, Kolli N, Tan SH, Anan T, Kametaka A, Azuma Y, Wilkinson KD, Dasso M (septembar 2006). "SUSP1 antagonizes formation of highly SUMO2/3-conjugated species" (PDF). The Journal of Cell Biology. 174 (7): 939–49. doi:10.1083/jcb.200510103. PMC 2064386. PMID 17000875.
- Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (novembar 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.