SFPQ

SFPQ
Identifikatori
Aliasi
Vanjski ID-jevi	GeneCards: [1]
Lokacija gena (čovjek)
Hrom.	Hromosom 16 (čovjek)
Kraj	84,197,053 bp
Obrazac RNK ekspresije
	; ; ; ;
	Više referentnih podataka o ekspresiji
Ortolozi
	654780
	n/a
	ENSG00000250685
	n/a
	n; a
	n/a
	NM_001039504
	n/a
	n/a
	n/a
	Wikipodaci
Pogledaj/uredi – čovjek

Prolinom i glutaminom bogati splajsing faktor jest protein koji je kod ljudi kodiran genom SFPQ sa hromosoma 1.^[3]^[4]

Proteini se često nazivaju u skladu s aktivnošću s kojom su prvi put povezani ili organelama u kojima su prvobitno identificirani. Međutim, različiti jedarni faktori djeluju u višestrukim molekulskim reakcijama koje se odvijaju istovremeno unutar jedra. U jednom pregledu iz 2002., opisuju se funkcije jedarnih faktora PSF (polipirimidinski trakt-vezujući proteinu pridruženi splajsing faktor) i p54nrb/NonO. PSF je prvobitno nazvan faktor prerade zbog njegove povezanosti s drugim korakom pre-iRNK prerade, dok se smatralo da p54nrb/NonO učestvuje u regulaciji transkripcije. Nedavni dokazi pokazuju da pojednostavljena kategorizacija PSF-a i njegovog homologa p54nrb/NonO na bilo koju pojedinačnu jedarnu aktivnost nije moguća i zapravo ovi proteini pokazuju multifunkcionalne karakteristike u različitim jedarnim procesima.

Jedroo je složena organela u kojoj se odvija širok spektar procesa. Ovim aktivnostima se pripisuju specifični jedarni faktori. Naprimjer, biti "faktor orerade" obično će značiti vezivanje za RNK i sudjelovanje u aktivnostima prerade RNK, dok će temin "transkripcijski faktor" insinuirati interakcije s DNK i regulaciju transkripcije. Međutim, jedarne reakcije se događaju istovremeno i u vrlo bliskoj blizini . Zapravo, postoji unakrsni odnos između jedarnih procesa i određeni proteini mogu sudjelovati u više jedarnih događaja. U pomenur+tom pregledu razmatrani su podaci o dva takva multifunkcionalna proteina, PSF i p54nrb/NonO.

PSF, polipeptid od 100 kDa, identificiran je i karakteriziran u kompleksu sa PTB-om. Polipirimidinski trakt je regija koja se nalazi u većini introna viših eukariota za koju se može vezati nekoliko faktora i važna je za definiciju mjesta prerade 3'. Međutim, većina PSF-a je povezana s jedarnim matriksom i nisu vezanz za PTB. Imunofluorescentno bojenje je pokazalo nukleoplazmatsku distribuciju PSF-a u difuznim i punktatnim obrascima, gdje je lokalizacija u nukleoplazmatskim žarištima određena prisustvom RNK motiva prepoznavanja (RRM). N-terminal PSF-a bogat prolinom/glutaminom može biti uključen u interakcije protein-protein.^[5]

Interakcije[uredi | uredi izvor]

Pokazano je da SFPQ ima interakcie sa PTBP1,^[3]^[6] NONO,^[7] CDC5L^[8] i ubikvitinom C.^[9]

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000250685 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ^a ^b Patton JG, Porro EB, Galceran J, Tempst P, Nadal-Ginard B (Mar 1993). "Cloning and characterization of PSF, a novel pre-mRNA splicing factor". Genes & Development. 7 (3): 393–406. doi:10.1101/gad.7.3.393. PMID 8449401.
^ "Entrez Gene: SFPQ splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated)".
^ Shav-Tal Y, Zipori D (Nov 2002). "PSF and p54(nrb)/NonO--multi-functional nuclear proteins". FEBS Letters. 531 (2): 109–14. doi:10.1016/S0014-5793(02)03447-6. PMID 12417296. S2CID 12485304.
^ Meissner M, Dechat T, Gerner C, Grimm R, Foisner R, Sauermann G (Jan 2000). "Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB". Journal of Cellular Biochemistry. 76 (4): 559–66. doi:10.1002/(SICI)1097-4644(20000315)76:4<559::AID-JCB4>3.0.CO;2-U. PMID 10653975.
^ Peng R, Dye BT, Pérez I, Barnard DC, Thompson AB, Patton JG (Oct 2002). "PSF and p54nrb bind a conserved stem in U5 snRNA". RNA. 8 (10): 1334–47. doi:10.1017/S1355838202022070. PMC 1370341. PMID 12403470.
^ Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (Dec 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC 305846. PMID 11101529.
^ Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (Sep 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923.

Dopunska literatura[uredi | uredi izvor]

Gower HJ, Moore SE, Dickson G, Elsom VL, Nayak R, Walsh FS (Apr 1989). "Cloning and characterization of a myoblast cell surface antigen defined by 24.1D5 monoclonal antibody". Development. 105 (4): 723–31. PMID 2480877.
Gozani O, Patton JG, Reed R (Jul 1994). "A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction". The EMBO Journal. 13 (14): 3356–67. doi:10.1002/j.1460-2075.1994.tb06638.x. PMC 395233. PMID 8045264.
Zhang WW, Zhang LX, Busch RK, Farrés J, Busch H (Feb 1993). "Purification and characterization of a DNA-binding heterodimer of 52 and 100 kDa from HeLa cells". The Biochemical Journal. 290 (1): 267–72. doi:10.1042/bj2900267. PMC 1132410. PMID 8439294.
Clark J, Lu YJ, Sidhar SK, Parker C, Gill S, Smedley D, Hamoudi R, Linehan WM, Shipley J, Cooper CS (Oct 1997). "Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma". Oncogene. 15 (18): 2233–9. doi:10.1038/sj.onc.1201394. PMID 9393982.
Teigelkamp S, Mundt C, Achsel T, Will CL, Lührmann R (Nov 1997). "The human U5 snRNP-specific 100-kD protein is an RS domain-containing, putative RNA helicase with significant homology to the yeast splicing factor Prp28p". RNA. 3 (11): 1313–26. PMC 1369570. PMID 9409622.
Straub T, Grue P, Uhse A, Lisby M, Knudsen BR, Tange TO, Westergaard O, Boege F (Oct 1998). "The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction". The Journal of Biological Chemistry. 273 (41): 26261–4. doi:10.1074/jbc.273.41.26261. PMID 9756848.
Lutz CS, Cooke C, O'Connor JP, Kobayashi R, Alwine JC (Dec 1998). "The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor". RNA. 4 (12): 1493–9. doi:10.1017/S1355838298981183. PMC 1369720. PMID 9848648.
Meissner M, Dechat T, Gerner C, Grimm R, Foisner R, Sauermann G (Jan 2000). "Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB". Journal of Cellular Biochemistry. 76 (4): 559–66. doi:10.1002/(SICI)1097-4644(20000315)76:4<559::AID-JCB4>3.0.CO;2-U. PMID 10653975.
Urban RJ, Bodenburg Y, Kurosky A, Wood TG, Gasic S (Jun 2000). "Polypyrimidine tract-binding protein-associated splicing factor is a negative regulator of transcriptional activity of the porcine p450scc insulin-like growth factor response element". Molecular Endocrinology. 14 (6): 774–82. doi:10.1210/me.14.6.774. PMID 10847580.
Straub T, Knudsen BR, Boege F (Jun 2000). "PSF/p54(nrb) stimulates "jumping" of DNA topoisomerase I between separate DNA helices". Biochemistry. 39 (25): 7552–8. doi:10.1021/bi992898e. PMID 10858305.
Akhmedov AT, Lopez BS (Aug 2000). "Human 100-kDa homologous DNA-pairing protein is the splicing factor PSF and promotes DNA strand invasion". Nucleic Acids Research. 28 (16): 3022–30. doi:10.1093/nar/28.16.3022. PMC 108454. PMID 10931916.
de Vries H, Rüegsegger U, Hübner W, Friedlein A, Langen H, Keller W (Nov 2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". The EMBO Journal. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMC 305781. PMID 11060040.
Dye BT, Patton JG (Feb 2001). "An RNA recognition motif (RRM) is required for the localization of PTB-associated splicing factor (PSF) to subnuclear speckles". Experimental Cell Research. 263 (1): 131–44. doi:10.1006/excr.2000.5097. PMID 11161712.
Mathur M, Tucker PW, Samuels HH (Apr 2001). "PSF is a novel corepressor that mediates its effect through Sin3A and the DNA binding domain of nuclear hormone receptors". Molecular and Cellular Biology. 21 (7): 2298–311. doi:10.1128/MCB.21.7.2298-2311.2001. PMC 86864. PMID 11259580.
Shav-Tal Y, Cohen M, Lapter S, Dye B, Patton JG, Vandekerckhove J, Zipori D (Aug 2001). "Nuclear relocalization of the pre-mRNA splicing factor PSF during apoptosis involves hyperphosphorylation, masking of antigenic epitopes, and changes in protein interactions". Molecular Biology of the Cell. 12 (8): 2328–40. doi:10.1091/mbc.12.8.2328. PMC 58597. PMID 11514619.
Zhang Z, Carmichael GG (Aug 2001). "The fate of dsRNA in the nucleus: a p54(nrb)-containing complex mediates the nuclear retention of promiscuously A-to-I edited RNAs". Cell. 106 (4): 465–75. doi:10.1016/S0092-8674(01)00466-4. PMID 11525732. S2CID 18799711.
Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI (Jan 2002). "Directed proteomic analysis of the human nucleolus". Current Biology. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. S2CID 14132033.
Sewer MB, Nguyen VQ, Huang CJ, Tucker PW, Kagawa N, Waterman MR (Apr 2002). "Transcriptional activation of human CYP17 in H295R adrenocortical cells depends on complex formation among p54(nrb)/NonO, protein-associated splicing factor, and SF-1, a complex that also participates in repression of transcription". Endocrinology. 143 (4): 1280–90. doi:10.1210/en.143.4.1280. PMID 11897684.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000250685 - Ensembl, maj 2017

[2] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid8449401-3] Patton JG, Porro EB, Galceran J, Tempst P, Nadal-Ginard B (Mar 1993). "Cloning and characterization of PSF, a novel pre-mRNA splicing factor". Genes & Development. 7 (3): 393–406. doi:10.1101/gad.7.3.393. PMID 8449401.

[entrez-4] "Entrez Gene: SFPQ splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated)".

[5] Shav-Tal Y, Zipori D (Nov 2002). "PSF and p54(nrb)/NonO--multi-functional nuclear proteins". FEBS Letters. 531 (2): 109–14. doi:10.1016/S0014-5793(02)03447-6. PMID 12417296. S2CID 12485304.

[pmid10653975-6] Meissner M, Dechat T, Gerner C, Grimm R, Foisner R, Sauermann G (Jan 2000). "Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB". Journal of Cellular Biochemistry. 76 (4): 559–66. doi:10.1002/(SICI)1097-4644(20000315)76:4<559::AID-JCB4>3.0.CO;2-U. PMID 10653975.

[pmid12403470-7] Peng R, Dye BT, Pérez I, Barnard DC, Thompson AB, Patton JG (Oct 2002). "PSF and p54nrb bind a conserved stem in U5 snRNA". RNA. 8 (10): 1334–47. doi:10.1017/S1355838202022070. PMC 1370341. PMID 12403470.

[pmid11101529-8] Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (Dec 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC 305846. PMID 11101529.

[pmid16169070-9] Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (Sep 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923.

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