TOM1L2

TOM1L2
Identifikatori
Aliasi	TOM1L2
Vanjski ID-jevi	OMIM: 615519 MGI: 2443306 HomoloGene: 44901 GeneCards: TOM1L2
Lokacija gena (čovjek)
Hrom.	Hromosom 17 (čovjek)
Kraj	17,972,422 bp
Lokacija gena (miš)
Hrom.	Hromosom 11 (miš)
Kraj	60,243,731 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• clathrin binding; • GO:0001948, GO:0016582 vezivanje za proteine; • protein kinase binding;
Ćelijska komponenta	• Egzosom; • intracellular anatomical structure;
Biološki proces	• protein transport; • intracellular protein transport; • negative regulation of mitotic nuclear division; • GO:0072468 Transdukcija signala; • GO:0015915 transport;
	Izvori:Amigo / QuickGO
Ortolozi
	146691
	216810
	ENSG00000175662
	ENSMUSG00000000538
	Q6ZVM7
	Q5SRX1
NM_001033551; NM_001082968; NM_001288786; NM_001288787; NM_001288788;
	NM_001288789; NM_144678; NM_001350331; NM_001350332; NM_001350333
	NM_001039092; NM_001039093; NM_153080; NM_001359810
NP_001028723; NP_001076437; NP_001275715; NP_001275716; NP_001275717;
	NP_001275718; NP_001337260; NP_001337261; NP_001337262
NP_001034181; NP_001034182; NP_694720; NP_001346739; NP_001390817;
	NP_001390820; NP_001390822; NP_001390824; NP_001390825
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

TOM1-oliki protein 2 je protein koji je kod ljudi kodiran genom TOM1L2.^[5]^[6]

Dužina polipeptidnog lanca je 507 aminokiselina , a molekulska težina 55.556^[7]

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10	20	30	40	50
MEFLLGNPFS	TPVGQCLEKA	TDGSLQSEDW	TLNMEICDII	NETEEGPKDA
IRALKKRLNG	NRNYREVMLA	LTVLETCVKN	CGHRFHILVA	NRDFIDSVLV
KIISPKNNPP	TIVQDKVLAL	IQAWADAFRS	SPDLTGVVHI	YEELKRKGVE
FPMADLDALS	PIHTPQRSVP	EVDPAATMPR	SQSQQRTSAG	SYSSPPPAPY
SAPQAPALSV	TGPITANSEQ	IARLRSELDV	VRGNTKVMSE	MLTEMVPGQE
DSSDLELLQE	LNRTCRAMQQ	RIVELISRVS	NEEVTEELLH	VNDDLNNVFL
RYERFERYRS	GRSVQNASNG	VLNEVTEDNL	IDLGPGSPAV	VSPMVGNTAP
PSSLSSQLAG	LDLGTESVSG	TLSSLQQCNP	RDGFDMFAQT	RGNSLAEQRK
TVTYEDPQAV	GGLASALDNR	KQSSEGIPVA	QPSVMDDIEV	WLRTDLKGDD
LEEGVTSEEF	DKFLEERAKA	AEMVPDLPSP	PMEAPAPASN	PSGRKKPERS
EDALFAL

TOM1L2 pripada porodici proteina povezanih s TOM1, koji su uključeni u [[endocitoza|endocitozugg. Ovi proteini imaju N-terminalni Vps27 / HGS (604375) / STAM (601899) (VHS) domen, nakon čega slijedi GGA / TOM1 (GAT) domenn (Katoh et al., 2006).

Kloniranje i ekspresija

Katoh etz al. (2006) klonirani su ljudski TOM1L2. Izvedeni protein od 457 aminokiselina ima N-terminalni VHS domen, centralni GAT domen i C-terminalni domen sa sekvencom f klatrinskog boksa.

Funkcija gena

Koristeći imunoprecipitaciju i Western blot analize, Katoh et al. (2006) pokazali su da su C-terminalni domeni TOM1L1 i TOM1L2 u interakciji sa teškim lancem klatrina (CLTC). GAT domeni TOM1L1 i TOM1L2 u interakciji su s endosomnim proteinom TOLLIP. Zaključili su da, zajedno s TOLLIP-om, proteini porodice TOM1 regrutuju klatrin na endosome i moduliraju endosomsku funkciju.

Patricia Hartz (2013) je mapirala gen TOM1L2 u hromosomsku regiju 17p11.2 , na osnovu poravnanja sekvence TOM1L2 (GenBank AK057308) sa genomskom sekvencom (GRCh37).^[8]

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000175662 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000000538 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Seranski P, Heiss NS, Dhorne-Pollet S, Radelof U, Korn B, Hennig S, Backes E, Schmidt S, Wiemann S, Schwarz CE, Lehrach H, Poustka A (maj 1999). "Transcription mapping in a medulloblastoma breakpoint interval and Smith-Magenis syndrome candidate region: identification of 53 transcriptional units and new candidate genes". Genomics. 56 (1): 1–11. doi:10.1006/geno.1998.5647. PMID 10036180.
^ "Entrez Gene: TOM1L2 target of myb1-like 2 (chicken)".
^ "UniProt, Q6ZVM7". Pristupljeno 14. 6. 2021.
^ Katoh, Y., Imakagura, H., Futatsumori, M., Nakayama, K. Recruitment of clathrin onto endosomes by the Tom1-Tollip complex. Biochem. Biophys. Res. Commun. 341: 143-149, 2006. PubMed: 16412388

Dopunska literatura[uredi | uredi izvor]

Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
Yu W, Andersson B, Worley KC, et al. (1997). "Large-Scale Concatenation cDNA Sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
Bi W, Yan J, Stankiewicz P, et al. (2002). "Genes in a Refined Smith-Magenis Syndrome Critical Deletion Interval on Chromosome 17p11.2 and the Syntenic Region of the Mouse". Genome Res. 12 (5): 713–28. doi:10.1101/gr.73702. PMC 186594. PMID 11997338.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Clark HF, Gurney AL, Abaya E, et al. (2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and Transmembrane Proteins: A Bioinformatics Assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Colland F, Jacq X, Trouplin V, et al. (2004). "Functional Proteomics Mapping of a Human Signaling Pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Zhang Y, Wolf-Yadlin A, Ross PL, et al. (2005). "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules". Mol. Cell. Proteomics. 4 (9): 1240–50. doi:10.1074/mcp.M500089-MCP200. PMID 15951569.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
Katoh Y, Imakagura H, Futatsumori M, Nakayama K (2006). "Recruitment of clathrin onto endosomes by the Tom1-Tollip complex". Biochem. Biophys. Res. Commun. 341 (1): 143–9. doi:10.1016/j.bbrc.2005.12.156. PMID 16412388.
Franco M, Furstoss O, Simon V, et al. (2006). "The Adaptor Protein Tom1L1 Is a Negative Regulator of Src Mitogenic Signaling Induced by Growth Factors". Mol. Cell. Biol. 26 (5): 1932–47. doi:10.1128/MCB.26.5.1932-1947.2006. PMC 1430241. PMID 16479011.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000175662 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000000538 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10036180-5] Seranski P, Heiss NS, Dhorne-Pollet S, Radelof U, Korn B, Hennig S, Backes E, Schmidt S, Wiemann S, Schwarz CE, Lehrach H, Poustka A (maj 1999). "Transcription mapping in a medulloblastoma breakpoint interval and Smith-Magenis syndrome candidate region: identification of 53 transcriptional units and new candidate genes". Genomics. 56 (1): 1–11. doi:10.1006/geno.1998.5647. PMID 10036180.

[entrez-6] "Entrez Gene: TOM1L2 target of myb1-like 2 (chicken)".

[UniProt-7] "UniProt, Q6ZVM7". Pristupljeno 14. 6. 2021.

[8] Katoh, Y., Imakagura, H., Futatsumori, M., Nakayama, K. Recruitment of clathrin onto endosomes by the Tom1-Tollip complex. Biochem. Biophys. Res. Commun. 341: 143-149, 2006. PubMed: 16412388

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