WARS2
Mitohondrijska triptofanil-tRNK sintetaza jest enzim koji je kod ljudi kodiran genom WARS2 sa hromosoma 1.[5][6][7]
Aminokiselinska sekvenca[uredi | uredi izvor]
Dužina polipeptidnog lanca je 360 aminokiselina, a molekulska težina 40.147 Da.[8]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MALHSMRKAR | ERWSFIRALH | KGSAAAPALQ | KDSKKRVFSG | IQPTGILHLG | ||||
| NYLGAIESWV | RLQDEYDSVL | YSIVDLHSIT | VPQDPAVLRQ | SILDMTAVLL | ||||
| ACGINPEKSI | LFQQSQVSEH | TQLSWILSCM | VRLPRLQHLH | QWKAKTTKQK | ||||
| HDGTVGLLTY | PVLQAADILL | YKSTHVPVGE | DQVQHMELVQ | DLAQGFNKKY | ||||
| GEFFPVPESI | LTSMKKVKSL | RDPSAKMSKS | DPDKLATVRI | TDSPEEIVQK | ||||
| FRKAVTDFTS | EVTYDPAGRA | GVSNIVAVHA | AVTGLSVEEV | VRRSAGMNTA | ||||
| RYKLAVADAV | IEKFAPIKRE | IEKLKLDKDH | LEKVLQIGSA | KAKELAYTVC | ||||
| QEVKKLVGFL |
Funkcija[uredi | uredi izvor]
Aminoacil-tRNK sintetaze kataliziraju aminoacilaciju tRNK pomoću njihove srodne aminokiseline. Zbog njihove centralne uloge u povezivanju aminokiselina sa nukleotidnim tripletnim sadržanim u tRNK, smatra se da su aminoacil-tRNK sintetaze među prvim proteinima koji su se pojavili u evoluciji. Postoje dva oblika triptofanil-tRNK sintetaze, citoplazmatski, zvani WARS i mitohondrijski oblik, zvani WARS2. Ovaj gen kodira mitohondrijsku triptofanil-tRNK sintetazu. Opisane su dije alternativne varijante transkripta koje kodiraju različite izoforme].[7]
Reference[uredi | uredi izvor]
- ^ a b c GRCh38: Ensembl release 89: ENSG00000116874 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000004233 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Martinez-Dominguez MT, Justesen J, Kruse TA, Hansen LL (Mar 1999). "Assignment of the human mitochondrial tryptophanyl-tRNA synthetase (WARS2) to 1p13.3→p13.1 by radiation hybrid mapping". Cytogenet Cell Genet. 83 (3–4): 249–50. doi:10.1159/000015196. PMID 10072595. S2CID 28931531.
- ^ Jorgensen R, Søgaard TM, Rossing AB, Martensen PM, Justesen J (Jul 2000). "Identification and characterization of human mitochondrial tryptophanyl-tRNA synthetase". J Biol Chem. 275 (22): 16820–6. doi:10.1074/jbc.275.22.16820. PMID 10828066.
- ^ a b "Entrez Gene: WARS2 tryptophanyl tRNA synthetase 2, mitochondrial".
- ^ "UniProt, Q9UGM6" (jezik: eng.). Pristupljeno 29. 11. 2021.CS1 održavanje: nepoznati jezik (link)
Dopunska literatura[uredi | uredi izvor]
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Liu J, Shue E, Ewalt KL, Schimmel P (2004). "A new gamma-interferon-inducible promoter and splice variants of an anti-angiogenic human tRNA synthetase". Nucleic Acids Res. 32 (2): 719–27. doi:10.1093/nar/gkh240. PMC 373357. PMID 14757836.
- Oh JH, Yang JO, Hahn Y, Kim MR, Byun SS, Jeon YJ, Kim JM, Song KS, Noh SM, Kim S, Yoo HS, Kim YS, Kim NS (2006). "Transcriptome analysis of human gastric cancer". Mamm. Genome. 16 (12): 942–54. doi:10.1007/s00335-005-0075-2. PMID 16341674. S2CID 69278.
- Guo LT, Chen XL, Zhao BT, Shi Y, Li W, Xue H, Jin YX (2007). "Human tryptophanyl-tRNA synthetase is switched to a tRNA-dependent mode for tryptophan activation by mutations at V85 and I311". Nucleic Acids Res. 35 (17): 5934–43. doi:10.1093/nar/gkm633. PMC 2034488. PMID 17726052.
Vanjski linkovi[uredi | uredi izvor]
- WARS2 lokacija ljudskog genoma UCSC Genome Browser.
WARS2 detalji ljudskog genoma u UCSC Genome Browser.