POLR3K

POLR3K
Identifikatori
Aliasi	POLR3K
Vanjski ID-jevi	OMIM: 606007 MGI: 1914255 HomoloGene: 5610 GeneCards: POLR3K
Lokacija gena (čovjek)
Hrom.	Hromosom 16 (čovjek)
Kraj	53,608 bp
Lokacija gena (miš)
Hrom.	Hromosom 2 (miš)
Kraj	181,512,623 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• nucleic acid binding; • DNA-directed 5'-3' RNA polymerase activity; • vezivanje iona cinka; • vezivanje iona metala; • RNA polymerase III activity;
Ćelijska komponenta	• RNA polymerase III complex; • citosol; • Jedarce; • jedro; • nukleoplazma;
Biološki proces	• immune system process; • defense response to virus; • transcription, DNA-templated; • transcription by RNA polymerase III; • Urođeni imunski sistem; • termination of RNA polymerase III transcription; • tRNA 3'-trailer cleavage; • positive regulation of type I interferon production;
	Izvori:Amigo / QuickGO
Ortolozi
	51728
	67005
	ENSG00000161980
	ENSMUSG00000038628
	Q9Y2Y1
	Q9CQZ7
	NM_016310
	NM_025901
	NP_057394
	NP_080177
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Podjedinica RPC10 DNK-usmjerene RNK-polymeraze III je enzim kojimje kod ljudi kodiran genom POLR3K.^[5]^[6]^[7]

Chedin et al. (1998) mapirali su ljudski gen POLR3K na hromosomu 16, anallizoom genomske sekvence.^[8] Stumpf (2021) mapirao je gen POLR3K na hromosomsku regiju 16p13.3, na osnovu poravnavanja sekvence POLR3K-a (GenBank BC011932) sa genomskom sekvencom (GRCh38).^[9]

Ovaj gen kodira malu esencijalnu podjedinicu RNK-polimeraze III, polimeraze odgovorne za sintezu transfernih i malih ribosomskih RNK u eukariota.

Karboksi-terminalni domen ove podjedinice ima visok stepen sličnosti sekvence sa karboksi-terminalnim domenom faktora elongacije RNK-polimeraze II. Ovu sličnost u sekvenci potkrepljuju funkcionalne studije koje pokazuju da je potrebna za pravilno zaustavljanje i prekid tokom transkripcije.^[7]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 108 aminokiselina, a molekulska težina 12.336 Da.^[10].

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10	20	30	40	50
MLLFCPGCGN	GLIVEEGQRC	HRFSCNTCPY	VHNITRKVTN	RKYPKLKEVD
DVLGGAAAWE	NVDSTAESCP	KCEHPRAYFM	QLQTRSADEP	MTTFYKCCNA
QCGHRWRD

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000161980 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000038628 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Chedin S, Riva M, Schultz P, Sentenac A, Carles C (Jan 1999). "The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination". Genes Dev. 12 (24): 3857–71. doi:10.1101/gad.12.24.3857. PMC 317263. PMID 9869639.
^ Spakovskii GV, Lebedenko EN (Mar 1999). "[Molecular identification and characteristics of hRPC11, the smallest specific subunit of human RNA polymerase III]". Bioorg Khim. 24 (11): 877–80. PMID 10079944.
^ ^a ^b "Entrez Gene: POLR3K polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa".
^ Chedin, S., Riva, M., Schultz, P., Sentenac, A., Carles, C. The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination. Genes Dev. 12: 3857-3871, 1998. PubMed: 9869639.
^ Stumpf, A. M. Personal Communication. Baltimore, Md. 05/04/2021.
^ "UniProt, Q9Y2Y1". Pristupljeno 9. 7. 2021.

Dopunska literatura[uredi | uredi izvor]

Jang KL, Collins MK, Latchman DS (1992). "The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC". J. Acquir. Immune Defic. Syndr. 5 (11): 1142–7. PMID 1403646.
Flint J, Thomas K, Micklem G, et al. (1997). "The relationship between chromosome structure and function at a human telomeric region". Nat. Genet. 15 (3): 252–7. doi:10.1038/ng0397-252. PMID 9054936. S2CID 20630444.
Daniels RJ, Peden JF, Lloyd C, et al. (2001). "Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16". Hum. Mol. Genet. 10 (4): 339–52. doi:10.1093/hmg/10.4.339. PMID 11157797.
Hu P, Wu S, Sun Y, et al. (2002). "Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits". Mol. Cell. Biol. 22 (22): 8044–55. doi:10.1128/MCB.22.22.8044-8055.2002. PMC 134740. PMID 12391170.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Martin J, Han C, Gordon LA, et al. (2005). "The sequence and analysis of duplication-rich human chromosome 16". Nature. 432 (7020): 988–94. doi:10.1038/nature03187. PMID 15616553.
De Gobbi M, Viprakasit V, Hughes JR, et al. (2006). "A regulatory SNP causes a human genetic disease by creating a new transcriptional promoter". Science. 312 (5777): 1215–7. doi:10.1126/science.1126431. PMID 16728641. S2CID 16044469.
Johnson SS, Zhang C, Fromm J, et al. (2007). "Mammalian Maf1 is a negative regulator of transcription by all three nuclear RNA polymerases". Mol. Cell. 26 (3): 367–79. doi:10.1016/j.molcel.2007.03.021. PMID 17499043.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000161980 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000038628 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9869639-5] Chedin S, Riva M, Schultz P, Sentenac A, Carles C (Jan 1999). "The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination". Genes Dev. 12 (24): 3857–71. doi:10.1101/gad.12.24.3857. PMC 317263. PMID 9869639.

[pmid10079944-6] Spakovskii GV, Lebedenko EN (Mar 1999). "[Molecular identification and characteristics of hRPC11, the smallest specific subunit of human RNA polymerase III]". Bioorg Khim. 24 (11): 877–80. PMID 10079944.

[entrez-7] "Entrez Gene: POLR3K polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa".

[8] Chedin, S., Riva, M., Schultz, P., Sentenac, A., Carles, C. The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination. Genes Dev. 12: 3857-3871, 1998. PubMed: 9869639.

[9] Stumpf, A. M. Personal Communication. Baltimore, Md. 05/04/2021.

[UniProt-10] "UniProt, Q9Y2Y1". Pristupljeno 9. 7. 2021.

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