API5

API5
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	3U0R, 3V6A
Identifikatori
Aliasi	API5
Vanjski ID-jevi	OMIM: 609774 MGI: 1888993 HomoloGene: 4809 GeneCards: API5
Lokacija gena (čovjek)
Hrom.	Hromosom 11 (čovjek)
Kraj	43,344,529 bp
Lokacija gena (miš)
Hrom.	Hromosom 2 (miš)
Kraj	94,268,481 bp
Ontologija gena
Molekularna funkcija	• fibroblast growth factor binding; • GO:0001948, GO:0016582 vezivanje za proteine; • vezivanje sa RNK;
Ćelijska komponenta	• citoplazma; • spliceosomal complex; • membrana; • jedro; • nuclear speck;
Biološki proces	• negative regulation of fibroblast apoptotic process; • negative regulation of apoptotic process; • GO:0097285 apoptoza;
	Izvori:Amigo / QuickGO
Ortolozi
	8539
	11800
	ENSG00000166181
	ENSMUSG00000027193
	Q9BZZ5
	O35841
	NM_001142930; NM_001142931; NM_001243747; NM_006595
	NM_007466; NM_001305258; NM_001349003
	NP_001136402; NP_001136403; NP_001230676; NP_006586
	NP_001292187; NP_031492; NP_001335932
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Ljudski gen API5 kodira protein apoptozni inhibitor 5.^[5]^[6]

Ovaj gen kodira inhibitorni protein apoptoze, čija ekspresija sprječava apoptozu nakon lišavanja faktora rasta. ovaj protein potiskuje apoptozu izazvanu transkripcijskim faktorom E2F1, a također stupa u interakciju i negativno regulira acinus, jedarni faktor koji je uključen u apopto fragmentacijutskku DNK . Njegovo iscrpljivanje pojačava citotoksično djelovanje hemoterapijskih lijekova. Kristalna struktura API5 pokazala je funkciju interakcije protein-protein.^[7]

Bolesti povezane sa API5 uključuju adenokarcinom debelog crijeva i rak grlića materice.

API5 funkcionira u eksportu jedarne iRNK.^[8]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 524 aminokiseline, a molekulska težina 59.005 Da.^[9].

10	20	30	40	50
MPTVEELYRN	YGILADATEQ	VGQHKDAYQV	ILDGVKGGTK	EKRLAAQFIP
KFFKHFPELA	DSAINAQLDL	CEDEDVSIRR	QAIKELPQFA	TGENLPRVAD
ILTQLLQTDD	SAEFNLVNNA	LLSIFKMDAK	GTLGGLFSQI	LQGEDIVRER
AIKFLSTKLK	TLPDEVLTKE	VEELILTESK	KVLEDVTGEE	FVLFMKILSG
LKSLQTVSGR	QQLVELVAEQ	ADLEQTFNPS	DPDCVDRLLQ	CTRQAVPLFS
KNVHSTRFVT	YFCEQVLPNL	GTLTTPVEGL	DIQLEVLKLL	AEMSSFCGDM
EKLETNLRKL	FDKLLEYMPL	PPEEAENGEN	AGNEEPKLQF	SYVECLLYSF
HQLGRKLPDF	LTAKLNAEKL	KDFKIRLQYF	ARGLQVYIRQ	LRLALQGKTG
EALKTEENKI	KVVALKITNN	INVLIKDLFH	IPPSYKSTVT	LSWKPVQKVE
IGQKRASEDT	TSGSPPKKSS	AGPKRDARQI	YNPPSGKYSS	NLGNFNYEQR
GAFRGSRGGR	GWGTRGNRSR	GRLY

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000166181 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000027193 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Tewari M, Yu M, Ross B, Dean C, Giordano A, Rubin R (septembar 1997). "AAC-11, a novel cDNA that inhibits apoptosis after growth factor withdrawal". Cancer Research. 57 (18): 4063–9. PMID 9307294.
^ "Entrez Gene: API5 apoptosis inhibitor 5".
^ Han BG, Kim KH, Lee SJ, Jeong KC, Cho JW, Noh KH, Kim TW, Kim SJ, Yoon HJ, Suh SW, Lee S, Lee BI (mart 2012). "Helical repeat structure of apoptosis inhibitor 5 reveals protein-protein interaction modules". The Journal of Biological Chemistry. 287 (14): 10727–37. doi:10.1074/jbc.M111.317594. PMC 3322819. PMID 22334682.
^ Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI (2020). "Regulation of mRNA Export Through API5 and Nuclear FGF2 Interaction". Nucleic Acids Res. 48 (11): 6340–6352. doi:10.1093/nar/gkaa335. PMC 7293033. PMID 32383752.
^ "UniProt, Q9BZZ5". Pristupljeno 15. 8. 2021.

Dopunska literatura[uredi | uredi izvor]

Gianfrancesco F, Esposito T, Ciccodicola A, D'Esposito M, Mazzarella R, D'Urso M, Forabosco A (1999). "Molecular cloning and fine mapping of API5L1, a novel human gene strongly related to an antiapoptotic gene". Cytogenetics and Cell Genetics. 84 (3–4): 164–6. doi:10.1159/000015247. PMID 10393420. S2CID 44601647.
Kim JW, Cho HS, Kim JH, Hur SY, Kim TE, Lee JM, Kim IK, Namkoong SE (april 2000). "AAC-11 overexpression induces invasion and protects cervical cancer cells from apoptosis". Laboratory Investigation; A Journal of Technical Methods and Pathology. 80 (4): 587–94. doi:10.1038/labinvest.3780008. PMID 10780674.
Van den Berghe L, Laurell H, Huez I, Zanibellato C, Prats H, Bugler B (novembar 2000). "FIF [fibroblast growth factor-2 (FGF-2)-interacting-factor], a nuclear putatively antiapoptotic factor, interacts specifically with FGF-2". Molecular Endocrinology. 14 (11): 1709–24. doi:10.1210/me.14.11.1709. PMID 11075807.
Sutherland HG, Mumford GK, Newton K, Ford LV, Farrall R, Dellaire G, Cáceres JF, Bickmore WA (septembar 2001). "Large-scale identification of mammalian proteins localized to nuclear sub-compartments". Human Molecular Genetics. 10 (18): 1995–2011. doi:10.1093/hmg/10.18.1995. PMID 11555636.
Li Z, Hu CY, Mo BQ, Xu JD, Zhao Y (april 2003). "[Effect of beta-carotene on gene expression of breast cancer cells]". AI Zheng = Aizheng = Chinese Journal of Cancer. 22 (4): 380–4. PMID 12703993.
Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (januar 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
Kim JE, Tannenbaum SR, White FM (2005). "Global phosphoproteome of HT-29 human colon adenocarcinoma cells". Journal of Proteome Research. 4 (4): 1339–46. doi:10.1021/pr050048h. PMID 16083285.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (novembar 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
Morris EJ, Michaud WA, Ji JY, Moon NS, Rocco JW, Dyson NJ (novembar 2006). "Functional identification of Api5 as a suppressor of E2F-dependent apoptosis in vivo". PLOS Genetics. 2 (11): e196. doi:10.1371/journal.pgen.0020196. PMC 1636698. PMID 17112319.
Han BG, Kim KH, Lee SJ, Jeong KC, Cho JW, Noh KH, Kim TW, Kim SJ, Yoon HJ, Suh SW, Lee S, Lee BI (2012). "Helical repeat structure of apoptosis inhibitor 5 reveals protein-protein interaction modules". J Biol Chem. 287 (14): 10727–37. doi:10.1074/jbc.M111.317594. PMC 3322819. PMID 22334682.
Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI (2020). "Regulation of mRNA Export Through API5 and Nuclear FGF2 Interaction". Nucleic Acids Res. 48 (11): 6340–6352. doi:10.1093/nar/gkaa335. PMC 7293033. PMID 32383752.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000166181 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000027193 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9307294-5] Tewari M, Yu M, Ross B, Dean C, Giordano A, Rubin R (septembar 1997). "AAC-11, a novel cDNA that inhibits apoptosis after growth factor withdrawal". Cancer Research. 57 (18): 4063–9. PMID 9307294.

[entrez-6] "Entrez Gene: API5 apoptosis inhibitor 5".

[7] Han BG, Kim KH, Lee SJ, Jeong KC, Cho JW, Noh KH, Kim TW, Kim SJ, Yoon HJ, Suh SW, Lee S, Lee BI (mart 2012). "Helical repeat structure of apoptosis inhibitor 5 reveals protein-protein interaction modules". The Journal of Biological Chemistry. 287 (14): 10727–37. doi:10.1074/jbc.M111.317594. PMC 3322819. PMID 22334682.

[8] Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI (2020). "Regulation of mRNA Export Through API5 and Nuclear FGF2 Interaction". Nucleic Acids Res. 48 (11): 6340–6352. doi:10.1093/nar/gkaa335. PMC 7293033. PMID 32383752.

[UniProt-9] "UniProt, Q9BZZ5". Pristupljeno 15. 8. 2021.

[1]

[2]

[3]

[4]

[5]

[6]

[7]

[8]

[9]