ARHGEF2

ARHGEF2
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	5EFX
Identifikatori
Aliasi	ARHGEF2
Vanjski ID-jevi	OMIM: 607560 MGI: 103264 HomoloGene: 3468 GeneCards: ARHGEF2
Lokacija gena (čovjek)
Hrom.	Hromosom 1 (čovjek)
Kraj	156,007,070 bp
Lokacija gena (miš)
Hrom.	Hromosom 3 (miš)
Kraj	88,555,359 bp
Obrazac RNK ekspresije
	; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• microtubule binding; • vezivanje iona cinka; • transcription factor binding; • vezivanje iona metala; • GO:0001948, GO:0016582 vezivanje za proteine; • guanyl-nucleotide exchange factor activity;
Ćelijska komponenta	• citoplazma; • Vezikula; • citosol; • Golđijev aparat; • projekcija ćelije; • membrana; • focal adhesion; • bicellular tight junction; • Diobeno vreteno; • ćelijska membrana; • dendritic shaft; • ruffle membrane; • međućelijske veze; • neuronal cell body; • Mikrotubula; • citoskelet; • GO:0016023 citoplazmatska vezikula; • podosome; • GO:0097483, GO:0097481 postsynaptic density; • GO:0009327 makromolekulani kompleks; • glutamatergic synapse; • postsynaptic density, intracellular component;
Biološki proces	• GO:0007243 intracellular signal transduction; • negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress; • negative regulation of necroptotic process; • actin filament organization; • immune system process; • negative regulation of neurogenesis; • cellular response to tumor necrosis factor; • Ćelijska dioba; • cellular response to muramyl dipeptide; • cellular hyperosmotic response; • negative regulation of microtubule depolymerization; • regulation of cell population proliferation; • positive regulation of NF-kappaB transcription factor activity; • negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; • GO:0000767 cell morphogenesis; • establishment of mitotic spindle orientation; • positive regulation of interleukin-6 production; • positive regulation of tumor necrosis factor production; • positive regulation of apoptotic process; • positive regulation of peptidyl-tyrosine phosphorylation; • ćelijski ciklus; • regulation of Rho protein signal transduction; • intracellular protein transport; • Urođeni imunski sistem; • regulation of small GTPase mediated signal transduction; • GO:0003257, GO:0010735, GO:1901228, GO:1900622, GO:1904488 positive regulation of transcription by RNA polymerase II; • negative regulation of podosome assembly; • G protein-coupled receptor signaling pathway; • positive regulation of neuron differentiation; • asymmetric neuroblast division; • positive regulation of neuron migration; • multicellular organism development; • nervous system development; • Ćelijska diferencijacija;
	Izvori:Amigo / QuickGO
Ortolozi
	9181
	16800
	ENSG00000116584
	ENSMUSG00000028059
	Q92974
	Q60875
NM_001162383; NM_001162384; NM_004723; NM_001350110; NM_001350111;
	NM_001350112
	NM_001198911; NM_001198912; NM_001198913; NM_008487; NM_001377126
NP_001155855; NP_001155856; NP_004714; NP_001337039; NP_001337040;
	NP_001337041
	NP_001185840; NP_001185841; NP_001185842; NP_032513; NP_001364055
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Rho guaninsko nukleotidni izmjenjivački faktor 2 jest protein koji je kod ljudi kodiran genom ARHGEF2 sa hromosoma 1.^[5]^[6]^[7]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 986 aminokiselina, a molekulska težina 111.543 Da.^[8]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MSRIESLTRA	RIDRSRELAS	KTREKEKMKE	AKDARYTNGH	LFTTISVSGM
TMCYACNKSI	TAKEALICPT	CNVTIHNRCK	DTLANCTKVK	QKQQKAALLK
NNTALQSVSL	RSKTTIRERP	SSAIYPSDSF	RQSLLGSRRG	RSSLSLAKSV
STTNIAGHFN	DESPLGLRRI	LSQSTDSLNM	RNRTLSVESL	IDEAEVIYSE
LMSDFEMDEK	DFAADSWSLA	VDSSFLQQHK	KEVMKQQDVI	YELIQTELHH
VRTLKIMTRL	FRTGMLEELH	LEPGVVQGLF	PCVDELSDIH	TRFLSQLLER
RRQALCPGST	RNFVIHRLGD	LLISQFSGPS	AEQMCKTYSE	FCSRHSKALK
LYKELYARDK	RFQQFIRKVT	RPAVLKRHGV	QECILLVTQR	ITKYPLLISR
ILQHSHGIEE	ERQDLTTALG	LVKELLSNVD	EGIYQLEKGA	RLQEIYNRMD
PRAQTPVPGK	GPFGREELLR	RKLIHDGCLL	WKTATGRFKD	VLVLLMTDVL
VFLQEKDQKY	IFPTLDKPSV	VSLQNLIVRD	IANQEKGMFL	ISAAPPEMYE
VHTASRDDRS	TWIRVIQQSV	RTCPSREDFP	LIETEDEAYL	RRIKMELQQK
DRALVELLRE	KVGLFAEMTH	FQAEEDGGSG	MALPTLPRGL	FRSESLESPR
GERLLQDAIR	EVEGLKDLLV	GPGVELLLTP	REPALPLEPD	SGGNTSPGVT
ANGEARTFNG	SIELCRADSD	SSQRDRNGNQ	LRSPQEEALQ	RLVNLYGLLH
GLQAAVAQQD	TLMEARFPEG	PERREKLCRA	NSRDGEAGRA	GAAPVAPEKQ
ATELALLQRQ	HALLQEELRR	CRRLGEERAT	EAGSLEARLR	ESEQARALLE
REAEEARRQL	AALGQTEPLP	AEAPWARRPV	DPRRRSLPAG	DALYLSFNPP
QPSRGTDRLD	LPVTTRSVHR	NFEDRERQEL	GSPEERLQDS	SDPDTGSEEE
GSSRLSPPHS	PRDFTRMQDI	PEETESRDGE	AVASES

Funkcija[uredi | uredi izvor]

Rho GTPaze imaju fundamentalnu ulogu u brojnim ćelijskim procesima koji su inicirani vanćelijskim stimulansima koji djeluju preko receptora vezanih za G-protein. Kodirani protein može formirati kompleks s G-proteinima i stimulirati signale zavisne o rho.^[7]

Interakcije[uredi | uredi izvor]

Pokazalo se da ARHGEF2 ima interakciju sa PAK1.^[9]

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000116584 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000028059 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Ren Y, Li R, Zheng Y, Busch H (Feb 1999). "Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases". J Biol Chem. 273 (52): 34954–60. doi:10.1074/jbc.273.52.34954. PMID 9857026.
^ Ishikawa K, Nagase T, Suyama M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Dec 1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (3): 169–76. doi:10.1093/dnares/5.3.169. PMID 9734811.
^ ^a ^b "Entrez Gene: ARHGEF2 rho/rac guanine nucleotide exchange factor (GEF) 2".
^ "UniProt, Q92974" (jezik: en.). Pristupljeno 7. 12. 2021.CS1 održavanje: nepoznati jezik (link)
^ Zenke FT, Krendel M, DerMardirossian C, King CC, Bohl BP, Bokoch GM (Apr 2004). "p21-activated kinase 1 phosphorylates and regulates 14-3-3 binding to GEF-H1, a microtubule-localized Rho exchange factor". J. Biol. Chem. 279 (18): 18392–400. doi:10.1074/jbc.M400084200. PMID 14970201.

Dopunska literatura[uredi | uredi izvor]

Ehrhardt A, Ehrhardt GR, Guo X, Schrader JW (2002). "Ras and relatives--job sharing and networking keep an old family together". Exp. Hematol. 30 (10): 1089–106. doi:10.1016/S0301-472X(02)00904-9. PMID 12384139.
Reddy AB, Chatterjee A, Rothblum LI, Black A, Busch H (1989). "Isolation and characterization of complementary DNA to proliferating cell nucleolar antigen P40". Cancer Res. 49 (7): 1763–7. PMID 2466560.
Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
Gao Y, Xing J, Streuli M, Leto TL, Zheng Y (2002). "Trp(56) of rac1 specifies interaction with a subset of guanine nucleotide exchange factors". J. Biol. Chem. 276 (50): 47530–41. doi:10.1074/jbc.M108865200. PMID 11595749.
Krendel M, Zenke FT, Bokoch GM (2002). "Nucleotide exchange factor GEF-H1 mediates cross-talk between microtubules and the actin cytoskeleton". Nat. Cell Biol. 4 (4): 294–301. doi:10.1038/ncb773. PMID 11912491. S2CID 25681168.
Brajenovic M, Joberty G, Küster B, Bouwmeester T, Drewes G (2004). "Comprehensive proteomic analysis of human Par protein complexes reveals an interconnected protein network". J. Biol. Chem. 279 (13): 12804–11. doi:10.1074/jbc.M312171200. PMID 14676191.
Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986.
Zenke FT, Krendel M, DerMardirossian C, King CC, Bohl BP, Bokoch GM (2004). "p21-activated kinase 1 phosphorylates and regulates 14-3-3 binding to GEF-H1, a microtubule-localized Rho exchange factor". J. Biol. Chem. 279 (18): 18392–400. doi:10.1074/jbc.M400084200. PMID 14970201.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660. S2CID 2371325.
Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
Callow MG, Zozulya S, Gishizky ML, Jallal B, Smeal T (2005). "PAK4 mediates morphological changes through the regulation of GEF-H1". J. Cell Sci. 118 (Pt 9): 1861–72. doi:10.1242/jcs.02313. PMID 15827085.
Aijaz S, D'Atri F, Citi S, Balda MS, Matter K (2005). "Binding of GEF-H1 to the tight junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition". Dev. Cell. 8 (5): 777–86. doi:10.1016/j.devcel.2005.03.003. PMID 15866167.
Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.

Vanjski linkovi[uredi | uredi izvor]

Lokacija ljudskog genoma ARHGEF2 i stranica sa detaljima o genu ARHGEF2 u UCSC Genome Browseru.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000116584 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000028059 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9857026-5] Ren Y, Li R, Zheng Y, Busch H (Feb 1999). "Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases". J Biol Chem. 273 (52): 34954–60. doi:10.1074/jbc.273.52.34954. PMID 9857026.

[pmid9734811-6] Ishikawa K, Nagase T, Suyama M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Dec 1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (3): 169–76. doi:10.1093/dnares/5.3.169. PMID 9734811.

[entrez-7] "Entrez Gene: ARHGEF2 rho/rac guanine nucleotide exchange factor (GEF) 2".

[UniProt-8] "UniProt, Q92974" (jezik: en.). Pristupljeno 7. 12. 2021.CS1 održavanje: nepoznati jezik (link)

[pmid14970201-9] Zenke FT, Krendel M, DerMardirossian C, King CC, Bohl BP, Bokoch GM (Apr 2004). "p21-activated kinase 1 phosphorylates and regulates 14-3-3 binding to GEF-H1, a microtubule-localized Rho exchange factor". J. Biol. Chem. 279 (18): 18392–400. doi:10.1074/jbc.M400084200. PMID 14970201.

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