CLSPN

CLSPN
Identifikatori
Aliasi	CLSPN
Vanjski ID-jevi	OMIM: 605434 MGI: 2445153 HomoloGene: 11138 GeneCards: CLSPN
Lokacija gena (čovjek)
Hrom.	Hromosom 1 (čovjek)
Kraj	35,769,978 bp
Lokacija gena (miš)
Hrom.	Hromosom 4 (miš)
Kraj	126,487,696 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• vezivanje sa DNK; • GO:0001948, GO:0016582 vezivanje za proteine; • anaphase-promoting complex binding;
Ćelijska komponenta	• jedro; • nukleoplazma; • Golđijev aparat;
Biološki proces	• Replikacija DNK; • ćelijski ciklus; • DNA damage checkpoint signaling; • mitotic DNA replication checkpoint signaling; • GO:0035404 peptidyl-serine phosphorylation; • activation of protein kinase activity; • cellular response to DNA damage stimulus; • GO:0100026 Popravka DNK; • protein deubiquitination; • mitotic G2 DNA damage checkpoint signaling;
	Izvori:Amigo / QuickGO
Ortolozi
	63967
	269582
	ENSG00000092853
	ENSMUSG00000042489
	Q9HAW4
	Q80YR7
	NM_001190481; NM_022111; NM_001330490
	NM_175554
	NP_001177410; NP_001317419; NP_071394
	NP_780763; NP_001392727; NP_001392728; NP_001392729; NP_001392730
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Klaspin jest protein koji je kod ljudi kodiran genom CLSPN sa hromosoma 1.^[5]^[6]^[7]

Amiokiselininska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 1.339 aminokiselina, a molekulska težina 151.094 Da.^[8]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MTGEVGSEVH	LEINDPNVIS	QEEADSPSDS	GQGSYETIGP	LSEGDSDEEI
FVSKKLKNRK	VLQDSDSETE	DTNASPEKTT	YDSAEEENKE	NLYAGKNTKI
KRIYKTVADS	DESYMEKSLY	QENLEAQVKP	CLELSLQSGN	STDFTTDRKS
SKKHIHDKEG	TAGKAKVKSK	RRLEKEERKM	EKIRQLKKKE	TKNQEDDVEQ
PFNDSGCLLV	DKDLFETGLE	DENNSPLEDE	ESLESIRAAV	KNKVKKHKKK
EPSLESGVHS	FEEGSELSKG	TTRKERKAAR	LSKEALKQLH	SETQRLIRES
ALNLPYHMPE	NKTIHDFFKR	KPRPTCHGNA	MALLKSSKYQ	SSHHKEIIDT
ANTTEMNSDH	HSKGSEQTTG	AENEVETNAL	PVVSKETQII	TGSDESCRKD
LVKNEELEIQ	EKQKQSDIRP	SPGDSSVLQQ	ESNFLGNNHS	EECQVGGLVA
FEPHALEGEG	PQNPEETDEK	VEEPEQQNKS	SAVGPPEKVR	RFTLDRLKQL
GVDVSIKPRL	GADEDSFVIL	EPETNRELEA	LKQRFWKHAN	PAAKPRAGQT
VNVNVIVKDM	GTDGKEELKA	DVVPVTLAPK	KLDGASHTKP	GEKLQVLKAK
LQEAMKLRRF	EERQKRQALF	KLDNEDGFEE	EEEEEEEMTD	ESEEDGEEKV
EKEEKEEELE	EEEEKEEEEE	EEGNQETAEF	LLSSEEIETK	DEKEMDKENN
DGSSEIGKAV	GFLSVPKSLS	SDSTLLLFKD	SSSKMGYFPT	EEKSETDENS
GKQPSKLDED	DSCSLLTKES	SHNSSFELIG	STIPSYQPCN	RQTGRGTSFF
PTAGGFRSPS	PGLFRASLVS	SASKSSGKLS	EPSLPIEDSQ	DLYNASPEPK
TLFLGAGDFQ	FCLEDDTQSQ	LLDADGFLNV	RNHRNQYQAL	KPRLPLASMD
ENAMDANMDE	LLDLCTGKFT	SQAEKHLPRK	SDKKENMEEL	LNLCSGKFTS
QDASTPASSE	LNKQEKESSM	GDPMEEALAL	CSGSFPTDKE	EEDEEEEFGD
FRLVSNDNEF	DSDEDEHSDS	GNDLALEDHE	DDDEEELLKR	SEKLKRQMRL
RKYLEDEAEV	SGSDVGSEDE	YDGEEIDEYE	EDVIDEVLPS	DEELQSQIKK
IHMKTMLDDD	KRQLRLYQER	YLADGDLHSD	GPGRMRKFRW	KNIDDASQMD
LFHRDSDDDQ	TEEQLDESEA	RWRKERIERE	QWLRDMAQQG	KITAEEEEEI
GEDSQFMILA	KKVTAKALQK	NASRPMVIQE	SKSLLRNPFE	AIRPGSAQQV
KTGSLLNQPK	AVLQKLAALS	DHNPSAPRNS	RNFVFHTLSP	VKAEAAKESS
KSQVKKRGPS	FMTSPSPKHL	KTDDSTSGLT	RSIFKYLES

Funkcija[uredi | uredi izvor]

Klaspin u žaba roda Xenopus je esencijalni uzvodni regulator kinazne kontrolne tačke 1 i pokreće zaustavljanje kontrolne tačke ćelijskog ciklusa u prisustvu DNK šablona u ekstraktima ksenopusovih jaja. Čini se da je ljudski gen homolog njegovom klaspinu, a njegova funkcija još nije utvrđena.^[7]

Interakcije[uredi | uredi izvor]

Pokazalo se da CLSPN ima interakcije sa:

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000092853 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000042489 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Kumagai A, Dunphy WG (Nov 2000). "Claspin, a novel protein required for the activation of Chk1 during a DNA replication checkpoint response in Xenopus egg extracts". Mol Cell. 6 (4): 839–49. doi:10.1016/S1097-2765(05)00092-4. PMID 11090622.
^ ^a ^b Chini CC, Chen J (Aug 2003). "Human claspin is required for replication checkpoint control". J Biol Chem. 278 (32): 30057–62. doi:10.1074/jbc.M301136200. PMID 12766152.
^ ^a ^b "Entrez Gene: CLSPN claspin homolog (Xenopus laevis)".
^ "UniProt, Q9HAW4" (jezik: engleski). Pristupljeno 23. 11. 2021.
^ Lin SY, Li K, Stewart GS, Elledge SJ (2004). "Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation". Proc. Natl. Acad. Sci. U.S.A. 101 (17): 6484–9. Bibcode:2004PNAS..101.6484L. doi:10.1073/pnas.0401847101. PMC 404071. PMID 15096610.
^ ^a ^b ^c Serçin O, Kemp MG (maj 2011). "Characterization of functional domains in human Claspin". Cell Cycle. 10 (10): 1599–606. doi:10.4161/cc.10.10.15562. PMC 3127160. PMID 21478680.
^ Faustrup H, Bekker-Jensen S, Bartek J, Lukas J, Mailand N (2009). "USP7 counteracts SCFbetaTrCP- but not APCCdh1-mediated proteolysis of Claspin". J. Cell Biol. 184 (1): 13–9. doi:10.1083/jcb.200807137. PMC 2615094. PMID 19124652.

Dopunska literatura[uredi | uredi izvor]

Chini CC, Chen J (2005). "Claspin, a regulator of Chk1 in DNA replication stress pathway". DNA Repair (Amst.). 3 (8–9): 1033–7. doi:10.1016/j.dnarep.2004.03.001. PMID 15279790.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Lin SY, Li K, Stewart GS, Elledge SJ (2004). "Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation". Proc. Natl. Acad. Sci. U.S.A. 101 (17): 6484–9. Bibcode:2004PNAS..101.6484L. doi:10.1073/pnas.0401847101. PMC 404071. PMID 15096610.
Sørensen CS, Syljuåsen RG, Lukas J, Bartek J (2006). "ATR, Claspin and the Rad9-Rad1-Hus1 complex regulate Chk1 and Cdc25A in the absence of DNA damage". Cell Cycle. 3 (7): 941–5. doi:10.4161/cc.3.7.972. PMID 15190204.
Sar F, Lindsey-Boltz LA, Subramanian D, et al. (2004). "Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures". J. Biol. Chem. 279 (38): 39289–95. doi:10.1074/jbc.M405793200. PMID 15226314.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Clarke CA, Clarke PR (2005). "DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system". Biochem. J. 388 (Pt 2): 705–12. doi:10.1042/BJ20041966. PMC 1138979. PMID 15707391.
Clarke CA, Bennett LN, Clarke PR (2005). "Cleavage of claspin by caspase-7 during apoptosis inhibits the Chk1 pathway". J. Biol. Chem. 280 (42): 35337–45. doi:10.1074/jbc.M506460200. PMID 16123041.
Chini CC, Wood J, Chen J (2006). "Chk1 is required to maintain claspin stability". Oncogene. 25 (30): 4165–71. doi:10.1038/sj.onc.1209447. PMID 16501606.
Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi:10.1038/nature04727. PMID 16710414.
Bennett LN, Clarke PR (2006). "Regulation of Claspin degradation by the ubiquitin-proteosome pathway during the cell cycle and in response to ATR-dependent checkpoint activation". FEBS Lett. 580 (17): 4176–81. doi:10.1016/j.febslet.2006.06.071. PMID 16828751.
Liu S, Bekker-Jensen S, Mailand N, et al. (2006). "Claspin operates downstream of TopBP1 to direct ATR signaling towards Chk1 activation". Mol. Cell. Biol. 26 (16): 6056–64. doi:10.1128/MCB.00492-06. PMC 1592810. PMID 16880517.
Mailand N, Bekker-Jensen S, Bartek J, Lukas J (2006). "Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress". Mol. Cell. 23 (3): 307–18. doi:10.1016/j.molcel.2006.06.016. PMID 16885021.
Peschiaroli A, Dorrello NV, Guardavaccaro D, et al. (2006). "SCFbetaTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response". Mol. Cell. 23 (3): 319–29. doi:10.1016/j.molcel.2006.06.013. PMID 16885022.
Mamely I, van Vugt MA, Smits VA, et al. (2006). "Polo-like kinase-1 controls proteasome-dependent degradation of Claspin during checkpoint recovery". Curr. Biol. 16 (19): 1950–5. doi:10.1016/j.cub.2006.08.026. PMID 16934469.
Rodríguez-Bravo V, Guaita-Esteruelas S, Florensa R, et al. (2007). "Chk1- and claspin-dependent but ATR/ATM- and Rad17-independent DNA replication checkpoint response in HeLa cells". Cancer Res. 66 (17): 8672–9. doi:10.1158/0008-5472.CAN-05-4443. PMID 16951182.
Chini CC, Chen J (2006). "Repeated phosphopeptide motifs in human Claspin are phosphorylated by Chk1 and mediate Claspin function". J. Biol. Chem. 281 (44): 33276–82. doi:10.1074/jbc.M604373200. PMID 16963448.
Sercin O, Kemp MG (2011). "Characterization of functional domains in human Claspin". Cell Cycle. 10 (10): 1599–606. doi:10.4161/cc.10.10.15562. PMC 3127160. PMID 21478680.

Vanjski linkovi[uredi | uredi izvor]

Lokacija ljudskog genoma CLSPN i stranica sa detaljima o genu CLSPN u UCSC Genome Browseru.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000092853 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000042489 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid11090622-5] Kumagai A, Dunphy WG (Nov 2000). "Claspin, a novel protein required for the activation of Chk1 during a DNA replication checkpoint response in Xenopus egg extracts". Mol Cell. 6 (4): 839–49. doi:10.1016/S1097-2765(05)00092-4. PMID 11090622.

[pmid12766152-6] Chini CC, Chen J (Aug 2003). "Human claspin is required for replication checkpoint control". J Biol Chem. 278 (32): 30057–62. doi:10.1074/jbc.M301136200. PMID 12766152.

[entrez-7] "Entrez Gene: CLSPN claspin homolog (Xenopus laevis)".

[UniProt-8] "UniProt, Q9HAW4" (jezik: engleski). Pristupljeno 23. 11. 2021.

[pmid15096610-9] Lin SY, Li K, Stewart GS, Elledge SJ (2004). "Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation". Proc. Natl. Acad. Sci. U.S.A. 101 (17): 6484–9. Bibcode:2004PNAS..101.6484L. doi:10.1073/pnas.0401847101. PMC 404071. PMID 15096610.

[pmid21478680-10] Serçin O, Kemp MG (maj 2011). "Characterization of functional domains in human Claspin". Cell Cycle. 10 (10): 1599–606. doi:10.4161/cc.10.10.15562. PMC 3127160. PMID 21478680.

[pmid19124652-11] Faustrup H, Bekker-Jensen S, Bartek J, Lukas J, Mailand N (2009). "USP7 counteracts SCFbetaTrCP- but not APCCdh1-mediated proteolysis of Claspin". J. Cell Biol. 184 (1): 13–9. doi:10.1083/jcb.200807137. PMC 2615094. PMID 19124652.

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