RETNLB

RETNLB
Identifikatori
Aliasi	RETNLB
Vanjski ID-jevi	OMIM: 605645 MGI: 1888505 HomoloGene: 10735 GeneCards: RETNLB
Lokacija gena (čovjek)
Hrom.	Hromosom 3 (čovjek)
Kraj	108,757,410 bp
Lokacija gena (miš)
Hrom.	Hromosom 16 (miš)
Kraj	48,639,255 bp
Ontologija gena
Molekularna funkcija	• hormone activity; • molekularna funkcija;
Ćelijska komponenta	• extracellular region; • Vanćelijsko; • ćelijska komponenta;
Biološki proces	• Ćelijska proliferacija; • regulation of signaling receptor activity; • GO:0072468 Transdukcija signala;
	Izvori:Amigo / QuickGO
Ortolozi
	84666
	57263
	ENSG00000163515
	ENSMUSG00000022650
	Q9BQ08
	Q99P86
	NM_032579
	NM_023881
	NP_115968
	NP_076370
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Rezistinoliki protein beta jest protein koji je kod ljudi kodiran genom RETNLB sa hromosoma 3.^[5]^[6]^[7]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 111 aminokiselina, а molekulska težina 11.730 Da.^[8]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MGPSSCLLLI	LIPLLQLINP	GSTQCSLDSV	MDKKIKDVLN	SLEYSPSPIS
KKLSCASVKS	QGRPSSCPAG	MAVTGCACGY	GCGSWDVQLE	TTCHCQCSVV
DWTTARCCHL	T

Funkcija[uredi | uredi izvor]

U nedavnoj studiji (2021) polazi se od činjnice da je karcinom usne šupljine po učestalosti šesti malignitet u svijetu, od kojih je oralni karcinom ploćasstih ćelija dominantan tip. Ova studija je imala za cilj da istraži funkciju i osnovni mehanizam rezistinu sličnog beta (RETNLB) u oralnom skvamoznom karcinomu. Podaci uzoraka oralnog skvamoznog karcinoma iz baze podataka Cancer Genome Atlas korišćeni su za ispitivanje ekspresije RETNLB i procenu njene korelacije sa kliničkim ishodima. Biološke funkcije RETNLB-a na rast, invaziju i migraciju ćelija određene su kompletom za brojanje ćelija 8, klonogenim rastom i Transwell testovima. Analiza obogaćivanja genskog skupa korištena je za identifikaciju važnih genskih skupova povezanih s ekspresijom RETNLB-s, što je dalje potvrđeno Western blot-om.

Otkrilisu da je RETNLB povećan u tkivima i pločastim ćelijama oralnog karcinoma. Visoka ekspresija RETNLB-a usko je povezana sa starošću i patološkim tumorom i značajno povezana sa lošim preživljavanjem pacijenata sa oralnim karcinomom skvamoznih ćelija. Daljnji funkcionalni eksperimenti pokazali su da nokdaun RETNLB-a značajno smanjuje održivost, pokretljivost i invazivnost ćelija. Štaviše, analiza obogaćivanja genskog skupa sugerira da je signalni put receptora sličan Tollu značajno povezano s visokom ekspresijom RETNLB. Daljnja Western blot analiza potvrdila je da utišavanje RETNLB-a može značajno potisnuti nivoe proteina Toll-likog receptora 2, Toll-likog receptora 4 i fosfor-vanćelijskim signalom regulirane kinaze.

Zaključeno je da ovi rezultati sugeriraju da smanjenje regulacije RETNLB-a može spriječiti progresiju oralnog karcinoma ploćastihih ćelija inaktivacijom TLR/2/4/ERK puta.^[9]

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000163515 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000022650 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Holcomb IN, Kabakoff RC, Chan B, Baker TW, Gurney A, Henzel W, Nelson C, Lowman HB, Wright BD, Skelton NJ, Frantz GD, Tumas DB, Peale FV Jr, Shelton DL, Hebert CC (Sep 2000). "FIZZ1, a novel cysteine-rich secreted protein associated with pulmonary inflammation, defines a new gene family". EMBO J. 19 (15): 4046–55. doi:10.1093/emboj/19.15.4046. PMC 306596. PMID 10921885.
^ Stutz AM, Pickart LA, Trifilieff A, Baumruker T, Prieschl-Strassmayr E, Woisetschlager M (Feb 2003). "The Th2 cell cytokines IL-4 and IL-13 regulate found in inflammatory zone 1/resistin-like molecule alpha gene expression by a STAT6 and CCAAT/enhancer-binding protein-dependent mechanism". J Immunol. 170 (4): 1789–96. doi:10.4049/jimmunol.170.4.1789. PMID 12574343.
^ "Entrez Gene: RETNLB resistin like beta".
^ "UniProt, Q9BQ08" (jezik: engleski). Pristupljeno 9. 11. 2021.
^ Hong Jin, Hui Miao, Yuan-Wen Nie, Yang-Yang Lin (2021): Investigating resistin like beta (RETNLB) as a tumor promoter for oral squamous cell carcinoma. Head Face Med 17(1):20; pmid: 34158059; pmcid: pmc8218422; doi: 10.1186/s13005-021-00272-4

Dopunska literatura[uredi | uredi izvor]

Kubota T, Kawano S, Chih DY, et al. (2001). "Representational difference analysis using myeloid cells from C/EBP epsilon deletional mice". Blood. 96 (12): 3953–7. doi:10.1182/blood.V96.12.3953. PMID 11090083.
Steppan CM, Brown EJ, Wright CM, et al. (2001). "A family of tissue-specific resistin-like molecules". Proc. Natl. Acad. Sci. U.S.A. 98 (2): 502–6. doi:10.1073/pnas.98.2.502. PMC 14616. PMID 11209052.
De Young MP, Damania H, Scheurle D, et al. (2003). "Bioinformatics-based discovery of a novel factor with apparent specificity to colon cancer". In Vivo. 16 (4): 239–48. PMID 12224133.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Clark HF, Gurney AL, Abaya E, et al. (2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and Transmembrane Proteins: A Bioinformatics Assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
He W, Wang ML, Jiang HQ, et al. (2003). "Bacterial colonization leads to the colonic secretion of RELMbeta/FIZZ2, a novel goblet cell-specific protein". Gastroenterology. 125 (5): 1388–97. doi:10.1016/j.gastro.2003.07.009. PMID 14598255.
Chumakov AM, Kubota T, Walter S, Koeffler HP (2004). "Identification of murine and human XCP1 genes as C/EBP-epsilon-dependent members of FIZZ/Resistin gene family". Oncogene. 23 (19): 3414–25. doi:10.1038/sj.onc.1207126. PMID 15064728.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Renigunta A, Hild C, Rose F, et al. (2006). "Human RELMbeta is a mitogenic factor in lung cells and induced in hypoxia". FEBS Lett. 580 (3): 900–3. doi:10.1016/j.febslet.2006.01.012. PMID 16427636. S2CID 42905763.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000163515 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000022650 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10921885-5] Holcomb IN, Kabakoff RC, Chan B, Baker TW, Gurney A, Henzel W, Nelson C, Lowman HB, Wright BD, Skelton NJ, Frantz GD, Tumas DB, Peale FV Jr, Shelton DL, Hebert CC (Sep 2000). "FIZZ1, a novel cysteine-rich secreted protein associated with pulmonary inflammation, defines a new gene family". EMBO J. 19 (15): 4046–55. doi:10.1093/emboj/19.15.4046. PMC 306596. PMID 10921885.

[pmid12574343-6] Stutz AM, Pickart LA, Trifilieff A, Baumruker T, Prieschl-Strassmayr E, Woisetschlager M (Feb 2003). "The Th2 cell cytokines IL-4 and IL-13 regulate found in inflammatory zone 1/resistin-like molecule alpha gene expression by a STAT6 and CCAAT/enhancer-binding protein-dependent mechanism". J Immunol. 170 (4): 1789–96. doi:10.4049/jimmunol.170.4.1789. PMID 12574343.

[entrez-7] "Entrez Gene: RETNLB resistin like beta".

[UniProt-8] "UniProt, Q9BQ08" (jezik: engleski). Pristupljeno 9. 11. 2021.

[9] Hong Jin, Hui Miao, Yuan-Wen Nie, Yang-Yang Lin (2021): Investigating resistin like beta (RETNLB) as a tumor promoter for oral squamous cell carcinoma. Head Face Med 17(1):20; pmid: 34158059; pmcid: pmc8218422; doi: 10.1186/s13005-021-00272-4

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