REXO2
REX2, RNK homolog egzonukleaze 2 (S. cerevisiae), znan i kao REXO2, je enzim koji je kod ljudi kodiran genom REXO2.[5][6][7]
Aminokiselinska sekvenca[uredi | uredi izvor]
Dužina polipeptidnog lanca je 237 aminokiselina, a molekulska težina 26.833 Da.[8]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MLGGSLGSRL | LRGVGGSHGR | FGARGVREGG | AAMAAGESMA | QRMVWVDLEM | ||||
| TGLDIEKDQI | IEMACLITDS | DLNILAEGPN | LIIKQPDELL | DSMSDWCKEH | ||||
| HGKSGLTKAV | KESTITLQQA | EYEFLSFVRQ | QTPPGLCPLA | GNSVHEDKKF | ||||
| LDKYMPQFMK | HLHYRIIDVS | TVKELCRRWY | PEEYEFAPKK | AASHRALDDI | ||||
| SESIKELQFY | RNNIFKKKID | EKKRKIIENG | ENEKTVS |
Funkcija[uredi | uredi izvor]
Nukleaze su komponente metabolizma DNK i RNK, koje obavljaju funkcije u popravljanju, replikaciji i rekombinaciji DNK te u preradi i razgradnji RNA. SFN je homolog Orn-a, 3-pra-u-5-pajmera egzoribonukleaza Escherichia coli koja napada slobodnu 3-primarnu hidroksilnu grupu na jednolančanoj RNK, oslobađajući 5-primarne mononukleotide na uzastopni način.[5]
Reference[uredi | uredi izvor]
- ^ a b c GRCh38: Ensembl release 89: ENSG00000076043 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000032026 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b "Entrez Gene: REXO2 REX2, RNA exonuclease 2 homolog (S. cerevisiae)".
- ^ Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W (maj 2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". Genome Res. 10 (5): 703–13. doi:10.1101/gr.10.5.703. PMC 310876. PMID 10810093.
- ^ Nguyen LH, Erzberger JP, Root J, Wilson DM (august 2000). "The human homolog of Escherichia coli Orn degrades small single-stranded RNA and DNA oligomers". J. Biol. Chem. 275 (34): 25900–6. doi:10.1074/jbc.M002672200. PMID 10851236.
- ^ "UniProt, Q9Y3B8". Pristupljeno 26. 8. 2021.
Dopunska literatura[uredi | uredi izvor]
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Lai CH, Chou CY, Ch'ang LY, et al. (2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". Genome Res. 10 (5): 703–13. doi:10.1101/gr.10.5.703. PMC 310876. PMID 10810093.
- Nguyen LH, Erzberger JP, Root J, Wilson DM (2000). "The human homolog of Escherichia coli Orn degrades small single-stranded RNA and DNA oligomers". J. Biol. Chem. 275 (34): 25900–6. doi:10.1074/jbc.M002672200. PMID 10851236.
- Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
- Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
- Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Ito S, Kita K, Zhai L, et al. (2006). "Involvement of human small fragment nuclease in the resistance of human cells to UV-C-induced cell death". Photochem. Photobiol. 80 (2): 281–5. doi:10.1562/2004-01-21-RA-051. PMID 15362935.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
- Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
- Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.