SHCBP1
SHCBP1 | |||||||||||||||||||||||||
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Identifikatori | |||||||||||||||||||||||||
Aliasi | SHCBP1 | ||||||||||||||||||||||||
Vanjski ID-jevi | OMIM: 611027 MGI: 1338802 HomoloGene: 32123 GeneCards: SHCBP1 | ||||||||||||||||||||||||
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Ortolozi | |||||||||||||||||||||||||
Vrste | Čovjek | Miš | |||||||||||||||||||||||
Entrez | |||||||||||||||||||||||||
Ensembl | |||||||||||||||||||||||||
UniProt | |||||||||||||||||||||||||
RefSeq (mRNK) | |||||||||||||||||||||||||
RefSeq (bjelančevina) | |||||||||||||||||||||||||
Lokacija (UCSC) | Chr 16: 46.58 – 46.62 Mb | Chr 8: 4.79 – 4.83 Mb | |||||||||||||||||||||||
PubMed pretraga | [3] | [4] | |||||||||||||||||||||||
Wikipodaci | |||||||||||||||||||||||||
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SHC SH2 domen-vezujuči protein 1 je protein koji je kod ljudi kodiran genom SHCBP1.
Međunarodni konzorcij za radijacijsko hibridno mapiranje lociralo je je gen SHCBP1 na hromosom 16 (RH78742).[5][6]
Aminokiselinska sekvenca[uredi | uredi izvor]
Dužina polipeptidnog lanca je 673 aminokiseline, a molekulska težina 75.690 Da.[7]
10 | 20 | 30 | 40 | 50 | ||||
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MADGSLTGGG | LEAAAMAPER | MGWAVEQELA | SLEKGLFQDE | DSCSDCSYRD | ||||
KPGSSLQSFM | PEGKTFFPEI | FQTNQLLFYE | RFRAYQDYIL | ADCKASEVQE | ||||
FTAEFLEKVL | EPSGWRAVWH | TNVFKVLVEI | TDVDFAALKA | VVRLAEPYLC | ||||
DSQVSTFTME | CMKELLDLKE | HRLPLQELWV | VFDDSGVFDQ | TALAIEHVRF | ||||
FYQNIWRSWD | EEEEDEYDYF | VRCVEPRLRL | HYDILEDRVP | SGLIVDYHNL | ||||
LSQCEESYRK | FLNLRSSLSN | CNSDSEQENI | SMVEGLKLYS | EMEQLKQKLK | ||||
LIENPLLRYV | FGYQKNSNIQ | AKGVRSSGQK | ITHVVSSTMM | AGLLRSLLTD | ||||
RLCQEPGEEE | REIQFHSDPL | SAINACFEGD | TVIVCPGHYV | VHGTFSIADS | ||||
IELEGYGLPD | DIVIEKRGKG | DTFVDCTGAD | IKISGIKFVQ | HDAVEGILIV | ||||
HRGKTTLENC | VLQCETTGVT | VRTSAEFLMK | NSDLYGAKGA | GIEIYPGSQC | ||||
TLSDNGIHHC | KEGILIKDFL | DEHYDIPKIS | MVNNIIHNNE | GYGVVLVKPT | ||||
IFSDLQENAE | DGTEENKALK | IQTSGEPDVA | ERVDLEELIE | CATGKMELCA | ||||
RTDPSEQVEG | NCEIVNELIA | ASTQKGQIKK | KRLSELGITQ | ADDNLMSQEM | ||||
FVGIVGNQFK | WNGKGSFGTF | LF |
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
Funkcija gena[uredi | uredi izvor]
Studijama koimunoprecipitacije na mišjima Shcbp1 i Shc, Schmandt et al. (1999) pokazali su da se Shcbp1 povezuje s izoformama p52 i p46 Shc. Studije padavina pokazale su da je povezanost Shcbp1 i Shc posredovana preko SH2 domena Shc i da je ta interakcija neovisna od fosforilacije tirozina.[8]
Kloniranje i ekspresija[uredi | uredi izvor]
2-hibridnim skriningom kvačeve biblioteka mišjih T-ćelija i embrionske cDNK s p52 SHC (SHC1) kao sondom, Schmandt et al. (1999) klonirali su Shcbp1, koji su nazvali Pal. Izvedeni mišji protein od 668 aminokiselina ima predviđenu molekulskuu masu od 75 kD i sadrži 23 ostatka tirozina, od kojih se nekoliko nalazi u motivima dobrog konsenzusa za domene SH2. Izolirali su ljudski SHCBP1, koji dijeli 78,2% aminokiselinskog identiteta sa svojim mišjim homologom. Northern blot analizom mišjih tkiva otkrivena je snažna ekspresija u sjemenicima i niža ekspresija u slezeni, plućima i srcu. Ekspresija iRNK Shcbp1 uočena je u mišićima u svim fazama razvoja embriona, a ekspresija iRNK Shcbp1 i proteina ograničena je na tkiva koja sadrže aktivno dijeleće ćelije i proliferirajuće ćelije u kulturi
Interakcije[uredi | uredi izvor]
Dokazano je da SHCBP1 ima interakcije sa SHC1.[5]
Reference[uredi | uredi izvor]
- ^ a b c GRCh38: Ensembl release 89: ENSG00000171241 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000022322 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b Schmandt R, Liu SK, McGlade CJ (Mar 1999). "Cloning and characterization of mPAL, a novel Shc SH2 domain-binding protein expressed in proliferating cells". Oncogene. 18 (10): 1867–1879. doi:10.1038/sj.onc.1202507. PMID 10086341.
- ^ "Entrez Gene: SHCBP1 SHC SH2-domain binding protein 1".
- ^ "UniProt, Q8NEM2". Pristupljeno 9. 7. 2021.
- ^ Schmandt, R., Liu, S. K., McGlade, C. J. Cloning and characterization of mPAL, a novel Shc SH2 domain-binding protein expressed in proliferating cells. Oncogene 18: 1867-1879, 1999. [ PubMed: 10086341
Dopunska literatura[uredi | uredi izvor]
- Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–174. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K (Feb 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase". Genes to Cells. 9 (2): 105–120. doi:10.1111/j.1365-2443.2004.00705.x. PMID 15009096. S2CID 21813024.
- Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–12135. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
- Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T (Aug 2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Current Biology. 14 (16): 1436–1450. doi:10.1016/j.cub.2004.07.051. PMID 15324660. S2CID 2371325.
- Benzinger A, Muster N, Koch HB, Yates JR, Hermeking H (Jun 2005). "Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer". Molecular & Cellular Proteomics. 4 (6): 785–795. doi:10.1074/mcp.M500021-MCP200. PMID 15778465.
- Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–1178. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
- Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP (Oct 2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nature Biotechnology. 24 (10): 1285–1292. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.
- Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.