VPS45
Vakuolski protein 45 pridružen sortiranju jest protein koji je kod ljudi kodiran genom VPS45 sa hromosoma 1.[5][6]
Aminokiselinska sekvenca[uredi | uredi izvor]
Dužina polipeptidnog lanca je 570 aminokiselina, a molekulska težina 65.077 Da.[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MNVVFAVKQY | ISKMIEDSGP | GMKVLLMDKE | TTGIVSMVYT | QSEILQKEVY | ||||
| LFERIDSQNR | EIMKHLKAIC | FLRPTKENVD | YIIQELRRPK | YTIYFIYFSN | ||||
| VISKSDVKSL | AEADEQEVVA | EVQEFYGDYI | AVNPHLFSLN | ILGCCQGRNW | ||||
| DPAQLSRTTQ | GLTALLLSLK | KCPMIRYQLS | SEAAKRLAEC | VKQVITKEYE | ||||
| LFEFRRTEVP | PLLLILDRCD | DAITPLLNQW | TYQAMVHELL | GINNNRIDLS | ||||
| RVPGISKDLR | EVVLSAENDE | FYANNMYLNF | AEIGSNIKNL | MEDFQKKKPK | ||||
| EQQKLESIAD | MKAFVENYPQ | FKKMSGTVSK | HVTVVGELSR | LVSERNLLEV | ||||
| SEVEQELACQ | NDHSSALQNI | KRLLQNPKVT | EFDAARLVML | YALHYERHSS | ||||
| NSLPGLMMDL | RNKGVSEKYR | KLVSAVVEYG | GKRVRGSDLF | SPKDAVAITK | ||||
| QFLKGLKGVE | NVYTQHQPFL | HETLDHLIKG | RLKENLYPYL | GPSTLRDRPQ | ||||
| DIIVFVIGGA | TYEEALTVYN | LNRTTPGVRI | VLGGTTVHNT | KSFLEEVLAS | ||||
| GLHSRSKESS | QVTSRSASRR |
Funkcija[uredi | uredi izvor]
Vezikulski posredovano sortiranje proteina ima važnu ulogu u segregaciji unutarćelijskihih molekula u različite organela. Genetičke studije kvasca identifikovale su više od 40 vakuokskih sortiranja proteina (VPS) gena uključenih u transport vezikula do vakuola. Ovaj gen je član porodice Sec1 domena i pokazuje visok stepen sličnosti sekvence sa mišjim, pacovskim i kvaščevim Vps45. Tačna funkcija ovog gena nije poznata, ali njegova visoka ekspresija u monjedarnim ćelijamama periferne krvi ukazuje na ulogu u prometu proteina, uključujući upalnene medijatore.[6]
Reference[uredi | uredi izvor]
- ^ a b c GRCh38: Ensembl release 89: ENSG00000136631 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000015747 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Pevsner J, Hsu SC, Hyde PS, Scheller RH (Feb 1997). "Mammalian homologues of yeast vacuolar protein sorting (vps) genes implicated in Golgi-to-lysosome trafficking". Gene. 183 (1–2): 7–14. doi:10.1016/S0378-1119(96)00367-8. PMID 8996080.
- ^ a b "Entrez Gene: VPS45 vacuolar protein sorting 45 homolog (S. cerevisiae)".
- ^ "UniProt, Q9NRW7" (jezik: en.). Pristupljeno 9. 12. 2021.CS1 održavanje: nepoznati jezik (link)
Dopunska literatura[uredi | uredi izvor]
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Tellam JT, James DE, Stevens TH, Piper RC (1997). "Identification of a mammalian Golgi Sec1p-like protein, mVps45". J. Biol. Chem. 272 (10): 6187–93. doi:10.1074/jbc.272.10.6187. PMID 9045632.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Rajasekariah P, Eyre HJ, Stanley KK, et al. (1999). "Molecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (h1Vps45)". Int. J. Biochem. Cell Biol. 31 (6): 683–94. doi:10.1016/S1357-2725(99)00017-5. PMID 10404641.
- Nielsen E, Christoforidis S, Uttenweiler-Joseph S, et al. (2000). "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain". J. Cell Biol. 151 (3): 601–12. doi:10.1083/jcb.151.3.601. PMC 2185588. PMID 11062261.
- de Renzis S, Sönnichsen B, Zerial M (2002). "Divalent Rab effectors regulate the sub-compartmental organization and sorting of early endosomes". Nat. Cell Biol. 4 (2): 124–33. doi:10.1038/ncb744. PMID 11788822. S2CID 6596498.
- Dulubova I, Yamaguchi T, Gao Y, et al. (2002). "How Tlg2p/syntaxin 16 'snares' Vps45". EMBO J. 21 (14): 3620–31. doi:10.1093/emboj/cdf381. PMC 126126. PMID 12110575.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Kim BY, Ueda M, Kominami E, et al. (2004). "Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex". Biochem. Biophys. Res. Commun. 311 (3): 577–82. doi:10.1016/j.bbrc.2003.10.030. PMID 14623309.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.