YBX2

YBX2
Identifikatori
Aliasi	YBX2
Vanjski ID-jevi	OMIM: 611447 MGI: 1096372 HomoloGene: 22942 GeneCards: YBX2
Lokacija gena (čovjek)
Hrom.	Hromosom 17 (čovjek)
Kraj	7,294,639 bp
Lokacija gena (miš)
Hrom.	Hromosom 11 (miš)
Kraj	69,941,605 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• vezivanje sa DNK; • vezivanje sa RNK; • nucleic acid binding; • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific;
Ćelijska komponenta	• citoplazma; • jedro; • fibrillar center;
Biološki proces	• GO:0009373 regulation of transcription, DNA-templated; • translational attenuation; • transcription by RNA polymerase II; • Spermatogeneza; • oocyte development; • GO:0044324, GO:0003256, GO:1901213, GO:0046019, GO:0046020, GO:1900094, GO:0061216, GO:0060994, GO:1902064, GO:0003258, GO:0072212 regulation of transcription by RNA polymerase II; • positive regulation of cold-induced thermogenesis;
	Izvori:Amigo / QuickGO
Ortolozi
	51087
	53422
	ENSG00000006047; ENSG00000288504
	ENSMUSG00000018554
	Q9Y2T7
	Q9Z2C8
	NM_015982
	NM_016875; NM_001347634
	NP_057066
	n/a
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Y-boksni protein 2 je protein koji je kod ljudi kodiran genom YBX2.^[5]^[6]^[7] YBX2 je specifičan za klicine ćelije iz porodice kičmenjačkih Y-boksnih proteina koji vežu nukleinske kiseline.

Dužina polipeptidnog lanca je 364 aminokiseline, a molekulska težina 38.518 Da.^[8].

PCR analizom s hibridima somatskih ćelija čovjeka-glodara, Tekur et al. (1999) mapirali su gen YBX2 u hromosomskoj sekvenci 17p13.1-p11.2.^[9]

Aminokiselinski sastav

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10	20	30	40	50
MSEVEAAAGA	TAVPAATVPA	TAAGVVAVVV	PVPAGEPQKG	GGAGGGGGAA
SGPAAGTPSA	PGSRTPGNPA	TAVSGTPAPP	ARSQADKPVL	AIQVLGTVKW
FNVRNGYGFI	NRNDTKEDVF	VHQTAIKRNN	PRKFLRSVGD	GETVEFDVVE
GEKGAEATNV	TGPGGVPVKG	SRYAPNRRKS	RRFIPRPPSV	APPPMVAEIP
SAGTGPGSKG	ERAEDSGQRP	RRWCPPPFFY	RRRFVRGPRP	PNQQQPIEGT
DRVEPKETAP	LEGHQQQGDE	RVPPPRFRPR	YRRPFRPRPR	QQPTTEGGDG
ETKPSQGPAD	GSRPEPQRPR	NRPYFQRRRQ	QAPGPQQAPG	PRQPAAPETS
APVNSGDPTT	TILE

Kloniranje i ekspresija[uredi | uredi izvor]

Preglednjem biblioteka cDNK ljudskog i mišjeg testisa Ybx2, nakon ponovnog skeniranja s pet osnovnih oligonukleotida iz pozitivnih klonova, Tekur et al. (1999) klonirali su YBX2, koji su nazvali contrin. Izvedeni protein s 364 aminokiseline ima izračunatu molekulsku masu od 38,6 kD i bogat je argininom i prolinom. Dijeli 69% i 73% aminokiselinskih identiteta s DBPA (CSDA), odnosno YB1 (NSEP1) i sadrži N-terminalni domen hladnog šoka (CSD), sedam navodnih mjesta fosforilacija za kazein-kinazu II, tri potencijalna mjesta fosforilacije za protein kinazu C i dvije signala jedarne lokalizacije unutar B/A otoka. Northern blot analizom otkriven je transkript od 1,8 kb samo u ljudskim testisima. YBX2 dijeli 93%, odnosno 77% aminokiselinskih identiteta s mišjim Ybx2 i ksenopusovim Frgy2.

Gu et al. (1998) klonirali su miša Ybx2, kojeg su nazvali Msy2, i alternativno preradili izoforme, Msy2a. Izvedene izoforme mišjeg Msy2 od 360 aminokiselina i Msy2a od 282 aminokiselina imale su izračunate molekulske mase od 38, odnosno 31 kD. Northern blot analizom mišjih tkiva, otkriven je Msy2 u zametnim ćelijama miša, samo s ekspresijom koja je prvi put otkrivena u testisima miševa starih 12 dana, povećavajući se značajno za 17 dana i dostigavši maksimalni nivo u testisima miša starim 22 dana , zadržavajući se u odrasloj dobi. Western blot analiza i imunohistokemijske studije otkrile su protein Msy2 u testisima miša te u oocitima u fazi diplotena i u zrelim. SDS-PAGE su pokazali da su izoforme mišjeg Msy2 i Msy2a migrirale s molekulskom masom od 52, odnosno 48 kD. Ova razlika u migraciji u odnosu na pravu molekulsku masu karakteristika je koja se dijeli s ostalim proteinima Y-boksa.

Koristeći imunohistohemijske studije, Kohno et al. (2006) otkrili su najviše nivoe DBPC u oocitima, spermatogonijama i spermatocitima sa umerenom ekspresijom u trofoblastima i niskim nivoima u plućima, srcu, kori nadbubrežne žlijezde i skeletnim mišićima. Suprotno ograničenoj distribuciji u normalnim ćelijama, pronašli su povećani nivo DBPC u različitim ljudskim karcinomima, uključujući adenokarcinome debelog crijeva (9/10) i holangiocelularne karcinome (8/10). Svi testirani tumori zametnih ćelija, uključujući seminome testisa i disgerminome jajnika, imunohistokemijskom analizom, pokazali su visok nivo DBPC.^[10]^[11]

Također pogledajte[uredi | uredi izvor]

Reference[uredi | uredi izvor]

^ ^a ^b ^c ENSG00000288504 GRCh38: Ensembl release 89: ENSG00000006047, ENSG00000288504 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000018554 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Tekur S, Pawlak A, Guellaen G, Hecht NB (Jul 1999). "Contrin, the human homologue of a germ-cell Y-box-binding protein: cloning, expression, and chromosomal localization". J Androl. 20 (1): 135–44. PMID 10100484.
^ Gu W, Tekur S, Reinbold R, Eppig JJ, Choi YC, Zheng JZ, Murray MT, Hecht NB (Dec 1998). "Mammalian male and female germ cells express a germ cell-specific Y-Box protein, MSY2". Biol Reprod. 59 (5): 1266–74. doi:10.1095/biolreprod59.5.1266. PMID 9780336.
^ "Entrez Gene: YBX2 Y box binding protein 2".
^ "UniProt, Q9Y2T7". Pristupljeno 14. 6. 2021.
^ Gu, W., Tekur, S., Reinbold, R., Eppig, J. J., Choi, Y.-C., Zheng, J. Z., Murray, M. T., Hecht, N. B. Mammalian male and female germ cells express a germ cell-specific Y-box protein, MSY2. Biol. Reprod. 59: 1266-1274, 1998. [PubMed]: 9780336
^ Kohno, Y., Matsuki, Y., Tanimoto, A., Izumi, H., Uchiumi, T., Kohno, K., Shimajiri, S., Sasaguri, Y. Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen. Brit. J. Cancer 94: 710-716, 2006. [PubMed]: 16479255.
^ Tekur, S., Pawlak, A., Guellaen, G., Hecht, N. B. Contrin, the human homologue of a germ-cell Y-box-binding protein: cloning, expression, and chromosomal localization. J. Androl. 20: 135-144, 1999. PubMed: 10100484

Dopunska literatura[uredi | uredi izvor]

Hammoud S, Emery BR, Dunn D, et al. (2009). "Sequence alterations in the YBX2 gene are associated with male factor infertility". Fertil. Steril. 91 (4): 1090–5. doi:10.1016/j.fertnstert.2008.01.009. PMID 18339382.
Yoshida T, Izumi H, Uchiumi T, et al. (2006). "Expression and cellular localization of dbpC/Contrin in germ cell tumor cell lines". Biochim. Biophys. Acta. 1759 (1–2): 80–8. doi:10.1016/j.bbaexp.2006.02.005. PMID 16624424.
Kohno Y, Matsuki Y, Tanimoto A, et al. (2006). "Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen". Br. J. Cancer. 94 (5): 710–6. doi:10.1038/sj.bjc.6602987. PMC 2361212. PMID 16479255.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.

[refGRCh38Ensembl-1] ENSG00000288504 GRCh38: Ensembl release 89: ENSG00000006047, ENSG00000288504 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000018554 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10100484-5] Tekur S, Pawlak A, Guellaen G, Hecht NB (Jul 1999). "Contrin, the human homologue of a germ-cell Y-box-binding protein: cloning, expression, and chromosomal localization". J Androl. 20 (1): 135–44. PMID 10100484.

[pmid9780336-6] Gu W, Tekur S, Reinbold R, Eppig JJ, Choi YC, Zheng JZ, Murray MT, Hecht NB (Dec 1998). "Mammalian male and female germ cells express a germ cell-specific Y-Box protein, MSY2". Biol Reprod. 59 (5): 1266–74. doi:10.1095/biolreprod59.5.1266. PMID 9780336.

[entrez-7] "Entrez Gene: YBX2 Y box binding protein 2".

[UniProt-8] "UniProt, Q9Y2T7". Pristupljeno 14. 6. 2021.

[9] Gu, W., Tekur, S., Reinbold, R., Eppig, J. J., Choi, Y.-C., Zheng, J. Z., Murray, M. T., Hecht, N. B. Mammalian male and female germ cells express a germ cell-specific Y-box protein, MSY2. Biol. Reprod. 59: 1266-1274, 1998. [PubMed]: 9780336

[10] Kohno, Y., Matsuki, Y., Tanimoto, A., Izumi, H., Uchiumi, T., Kohno, K., Shimajiri, S., Sasaguri, Y. Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen. Brit. J. Cancer 94: 710-716, 2006. [PubMed]: 16479255.

[11] Tekur, S., Pawlak, A., Guellaen, G., Hecht, N. B. Contrin, the human homologue of a germ-cell Y-box-binding protein: cloning, expression, and chromosomal localization. J. Androl. 20: 135-144, 1999. PubMed: 10100484

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