RPL27A
Podjedinica 60S ribosomskog proteina L27a je protein koji je kod ljudi kodiran genom RPL27A.[5][6][7]
Ribosomi, organele koje kataliziraju sintezu proteina, sastoje se od male 40S i velike 60S podjedinice. Ove podjedinice zajedno se sastoje od četiri tipa RNK i približno 80 strukturno različitih proteina. Ovaj gen kodira ribosomni protein koji je komponenta podjedinice 60S. Ovaj protein pripada porodici L15P ribosomnih proteina. Nalazi se u citoplazmi. Uočena je varijabilna ekspresija ovog gena u kolorekttumskom karcinomu u poređenju sa susjednim normalnim tkivima, iako nije pronađena korelacija između nivoa ekspresije i težine bolesti. Kao što je tipski za gene koji kodiraju ribosomske proteine, više obrađenih pseudogena izvedenih iz ovog gena raspršeno je širom genoma.[7]
Aminokiaelinska sekvenca[uredi | uredi izvor]
Dužina polipeptidnog lanca je 148 aminokiselina, а molekulska težina 16.561 Da.[8]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MPSRLRKTRK | LRGHVSHGHG | RIGKHRKHPG | GRGNAGGLHH | HRINFDKYHP | ||||
| GYFGKVGMKH | YHLKRNQSFC | PTVNLDKLWT | LVSEQTRVNA | AKNKTGAAPI | ||||
| IDVVRSGYYK | VLGKGKLPKQ | PVIVKAKFFS | RRAEEKIKSV | GGACVLVA |
- C: Cistein
- D: Asparaginska kiselina
- E: Glutaminska kiselina
- F: Fenilalanin
- G: Glicin
- H: Histidin
- I: Izoleucin
- K: Lizin
- L: Leucin
- M: Metionin
- N: Asparagin
- P: Prolin
- Q: Glutamin
- R: Arginin
- S: Serin
- T: Treonin
- V: Valin
- W: Triptofan
- Y: Tirozin
Reference[uredi | uredi izvor]
- ^ a b c GRCh38: Ensembl release 89: ENSG00000166441 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000046364 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Frigerio JM, Dagorn JC, Iovanna JL (Jul 1995). "Cloning, sequencing and expression of the L5, L21, L27a, L28, S5, S9, S10 and S29 human ribosomal protein mRNAs". Biochim Biophys Acta. 1262 (1): 64–8. doi:10.1016/0167-4781(95)00045-i. PMID 7772601.
- ^ Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (Aug 1998). "A map of 75 human ribosomal protein genes". Genome Res. 8 (5): 509–23. doi:10.1101/gr.8.5.509. PMID 9582194.
- ^ a b "Entrez Gene: RPL27A ribosomal protein L27a".
- ^ "UniProt, P46776". Pristupljeno 26. 8. 2021.
Dopunska literatura[uredi | uredi izvor]
- Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal proteins". Biochem. Cell Biol. 73 (11–12): 933–947. doi:10.1139/o95-101. PMID 8722009.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–2127. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–45. doi:10.1038/ng1285. PMID 14702039.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–16903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. S2CID 14132033.
- Bortoluzzi S, d'Alessi F, Romualdi C, Danieli GA (2002). "Differential expression of genes coding for ribosomal proteins in different human tissues". Bioinformatics. 17 (12): 1152–1157. doi:10.1093/bioinformatics/17.12.1152. PMID 11751223.
- Kusuda J, Hirai M, Tanuma R, et al. (1999). "Genomic structure and chromosome location of RPL27A/Rpl27a, the genes encoding human and mouse ribosomal protein L27A". Cytogenet. Cell Genet. 85 (3–4): 248–251. doi:10.1159/000015303. PMID 10449908. S2CID 7314458.