SPACA3

SPACA3
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	4YF2
Identifikatori
Aliasi	SPACA3
Vanjski ID-jevi	OMIM: 612749 MGI: 1922872 HomoloGene: 18365 GeneCards: SPACA3
Lokacija gena (čovjek)
Hrom.	Hromosom 17 (čovjek)
Kraj	32,997,877 bp
Lokacija gena (miš)
Hrom.	Hromosom 11 (miš)
Kraj	80,758,640 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• GO:0001948, GO:0016582 vezivanje za proteine; • lysozyme activity;
Ćelijska komponenta	• integral component of membrane; • extracellular region; • Akrozom; • secretory granule; • membrana; • GO:0016023 citoplazmatska vezikula; • Vanćelijsko; • acrosomal membrane; • acrosomal matrix;
Biološki proces	• monocyte activation; • cell wall macromolecule catabolic process; • oplodnja; • positive regulation of phagocytosis; • response to virus; • positive regulation of macrophage activation; • sperm-egg recognition; • GO:0051637 defense response to Gram-positive bacterium; • peptidoglycan catabolic process; • GO:0051636 defense response to Gram-negative bacterium; • fusion of sperm to egg plasma membrane involved in single fertilization;
	Izvori:Amigo / QuickGO
Ortolozi
	124912
	75622
	ENSG00000141316
	ENSMUSG00000053184
	Q8IXA5
	Q9D9X8
	NM_173847; NM_001317225; NM_001317226
	NM_029367; NM_001359183
	NP_001304154; NP_001304155; NP_776246
	NP_083643; NP_001346112
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Protein 3 spermatozoidno-akrosomske membrane je protein koki je kod ljudi kodiran genom SPACA3. Može biti uključen u prianjanje za jajnu ćeliju prije nego što se oplodi.^[5]^[6]

Dužina polipeptidnog lanca je 215 aminokiselina, a molekulska težina 23.431.^[7].

Aminokiselinska sekvenca

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10	20	30	40	50
MVSALRGAPL	IRVHSSPVSS	PSVSGPRRLV	SCLSSQSSAL	SQSGGGSTSA
AGIEARSRAL	RRRWCPAGIM	LLALVCLLSC	LLPSSEAKLY	GRCELARVLH
DFGLDGYRGY	SLADWVCLAY	FTSGFNAAAL	DYEADGSTNN	GIFQINSRRW
CSNLTPNVPN	VCRMYCSDLL	NPNLKDTVIC	AMKITQEPQG	LGYWEAWRHH
CQGKDLTEWV	DGCDF

Kloniranje i ekspresija[uredi | uredi izvor]

Proteasomskom analizom 2D gela ljudske sperme, nakon čega slijedi RACE-PCR ljudske testisne cDNK, Mandal et al. (2003) klonirali su SPACA3, koji su nazvali SLLP1, i identificirali homologe miša i pacova. Izvedeni protein SPACA3 od 215 aminokiselina ima izračunatu molekulsku masu od 23,4 kD. SPACA3 sadrži pretpostavljenu transmembransku regiju, praćenu predviđenim mjestom cijepanja proteazama između ala-lys veze, sekvencu transkripta za alfa-laktalbumin/lizozim C (LYZ) porodice proteina, tri predviđena N-terminala, jedno C-terminalno mjesto miristoilacije i dva mjesta fosforilacija. SPACA3 dijeli 53% i 52% identiteta s lizozimom želuca majmuna, odnosno s lizozimom čovjeka. Northern blot analiza ljudskih tkiva otkrila je transkript od 1,0 kb samo u testisu. Imunofluorescentne studije lokalizirale su SPACA3 na akrosomu i ekvatorskom segmentu glave spermatozoida, a imunozlatno bojenje otkrilo je distribuciju SPACA3 na cijeloj akrosomskoj matrici, uključujući glavni i ekvatorski segment.

Mandal et al. (2003) nisu pronašli dokaze o SPACA3 bakteriolitskoj aktivnosti, a SPACA3 nije pokazao značajniji efekat na vezanje i fuziju ljudske spermatozoida na jajima hrčaka. Utvrdili su da gen SPACA3 sadrži pet egzona u rasponu od oko 6,0 kb. Analizom genomske sekvence, mapirali su gen SPACA3 u hromosomskoj regiji 17q11.2. Zhang et al . (2005) mapirali su gen SPACA3 na 17q12.^[8]^[9]

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000141316 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000053184 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Mandal A, Klotz KL, Shetty J, Jayes FL, Wolkowicz MJ, Bolling LC, Coonrod SA, Black MB, Diekman AB, Haystead TA, Flickinger CJ, Herr JC (Apr 2003). "SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa". Biol Reprod. 68 (5): 1525–37. doi:10.1095/biolreprod.102.010108. PMID 12606493.
^ "Entrez Gene: SPACA3 sperm acrosome associated 3".
^ "UniProt, Q8IXA5". Pristupljeno 12. 6. 2021.
^ Mandal, A., Klotz, K. L., Shetty, J., Jayes, F. L., Wolkowicz, M. J., Bolling, L. C., Coonrod, S. A., Black, M. B., Diekman, A. B., Haystead, T. A. J., Flickinger, C. J., Herr, J. C. SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa. Biol. Reprod. 68: 1525-1537, 2003. [PubMed]: 12606493
^ Zhang, K., Gao, R., Zhang, H., Cai, X., Shen, C., Wu, C., Zhao, S., Yu, L. Molecular cloning and characterization of three novel lysozyme-like genes, predominantly expressed in the male reproductive system of humans, belonging to the C-type lysozyme/alpha-lactalbumin family. Biol. Reprod. 73: 1064-1071, 2005. [PubMed]: 16014814

Dopunska literatura[uredi | uredi izvor]

Niyonsaba F, Ogawa H (2006). "Protective roles of the skin against infection: implication of naturally occurring human antimicrobial agents beta-defensins, cathelicidin LL-37 and lysozyme". J. Dermatol. Sci. 40 (3): 157–68. doi:10.1016/j.jdermsci.2005.07.009. PMID 16150577.
Cramer EM, Breton-Gorius J (1987). "Ultrastructural localization of lysozyme in human neutrophils by immunogold". J. Leukoc. Biol. 41 (3): 242–7. doi:10.1002/jlb.41.3.242. PMID 3470418. S2CID 32012371.
Welsh IR, Spitznagel JK (1973). "Distribution of lysosomal enzymes, cationic proteins, and bactericidal substances in subcellular fractions of human polymorphonuclear leukocytes". Infect. Immun. 4 (2): 97–102. doi:10.1128/IAI.4.2.97-102.1971. PMC 416271. PMID 5154881.
Ibrahim HR, Matsuzaki T, Aoki T (2001). "Genetic evidence that antibacterial activity of lysozyme is independent of its catalytic function". FEBS Lett. 506 (1): 27–32. doi:10.1016/S0014-5793(01)02872-1. PMID 11591365. S2CID 21593262.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Clark HF, Gurney AL, Abaya E, et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
Wang Z, Zhang Y, Mandal A, et al. (2005). "The spermatozoa protein, SLLP1, is a novel cancer-testis antigen in hematologic malignancies". Clin. Cancer Res. 10 (19): 6544–50. doi:10.1158/1078-0432.CCR-04-0911. PMID 15475442.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Zhang K, Gao R, Zhang H, et al. (2006). "Molecular cloning and characterization of three novel lysozyme-like genes, predominantly expressed in the male reproductive system of humans, belonging to the c-type lysozyme/alpha-lactalbumin family". Biol. Reprod. 73 (5): 1064–71. doi:10.1095/biolreprod.105.041889. PMID 16014814. S2CID 21603980.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000141316 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000053184 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid12606493-5] Mandal A, Klotz KL, Shetty J, Jayes FL, Wolkowicz MJ, Bolling LC, Coonrod SA, Black MB, Diekman AB, Haystead TA, Flickinger CJ, Herr JC (Apr 2003). "SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa". Biol Reprod. 68 (5): 1525–37. doi:10.1095/biolreprod.102.010108. PMID 12606493.

[entrez-6] "Entrez Gene: SPACA3 sperm acrosome associated 3".

[UniProt-7] "UniProt, Q8IXA5". Pristupljeno 12. 6. 2021.

[8] Mandal, A., Klotz, K. L., Shetty, J., Jayes, F. L., Wolkowicz, M. J., Bolling, L. C., Coonrod, S. A., Black, M. B., Diekman, A. B., Haystead, T. A. J., Flickinger, C. J., Herr, J. C. SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa. Biol. Reprod. 68: 1525-1537, 2003. [PubMed]: 12606493

[9] Zhang, K., Gao, R., Zhang, H., Cai, X., Shen, C., Wu, C., Zhao, S., Yu, L. Molecular cloning and characterization of three novel lysozyme-like genes, predominantly expressed in the male reproductive system of humans, belonging to the C-type lysozyme/alpha-lactalbumin family. Biol. Reprod. 73: 1064-1071, 2005. [PubMed]: 16014814

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