USP1
Ubikvitin karboksil-terminalna hidrolaza 1 jest enzim koji je kod ljudi kodiran genom USP1 sa hromosoma 1.[5][6]
Aminokiselinska sekvenca[uredi | uredi izvor]
Dužina polipeptidnog lanca je 785 aminokiselina, a molekulska težina 88.207 Da.[7].
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MPGVIPSESN | GLSRGSPSKK | NRLSLKFFQK | KETKRALDFT | DSQENEEKAS | ||||
| EYRASEIDQV | VPAAQSSPIN | CEKRENLLPF | VGLNNLGNTC | YLNSILQVLY | ||||
| FCPGFKSGVK | HLFNIISRKK | EALKDEANQK | DKGNCKEDSL | ASYELICSLQ | ||||
| SLIISVEQLQ | ASFLLNPEKY | TDELATQPRR | LLNTLRELNP | MYEGYLQHDA | ||||
| QEVLQCILGN | IQETCQLLKK | EEVKNVAELP | TKVEEIPHPK | EEMNGINSIE | ||||
| MDSMRHSEDF | KEKLPKGNGK | RKSDTEFGNM | KKKVKLSKEH | QSLEENQRQT | ||||
| RSKRKATSDT | LESPPKIIPK | YISENESPRP | SQKKSRVKIN | WLKSATKQPS | ||||
| ILSKFCSLGK | ITTNQGVKGQ | SKENECDPEE | DLGKCESDNT | TNGCGLESPG | ||||
| NTVTPVNVNE | VKPINKGEEQ | IGFELVEKLF | QGQLVLRTRC | LECESLTERR | ||||
| EDFQDISVPV | QEDELSKVEE | SSEISPEPKT | EMKTLRWAIS | QFASVERIVG | ||||
| EDKYFCENCH | HYTEAERSLL | FDKMPEVITI | HLKCFAASGL | EFDCYGGGLS | ||||
| KINTPLLTPL | KLSLEEWSTK | PTNDSYGLFA | VVMHSGITIS | SGHYTASVKV | ||||
| TDLNSLELDK | GNFVVDQMCE | IGKPEPLNEE | EARGVVENYN | DEEVSIRVGG | ||||
| NTQPSKVLNK | KNVEAIGLLG | GQKSKADYEL | YNKASNPDKV | ASTAFAENRN | ||||
| SETSDTTGTH | ESDRNKESSD | QTGINISGFE | NKISYVVQSL | KEYEGKWLLF | ||||
| DDSEVKVTEE | KDFLNSLSPS | TSPTSTPYLL | FYKKL |
Funkcija[uredi | uredi izvor]
Ovaj gen kodira člana porodice ubikvitin-specifične obrade (UBP) proteaza koji je deubikvitinirajući enzim (DUB) sa His i Cys domenima. Ovaj protein nalazi se u citoplazmi i cijepa ubikvitinski dio prekursora spojenih na ubikvitin i ubikvitiniliranih proteina.
Protein specifično deubikvitinira protein u putu popravke DNK Fanconijeve anemije (FA). Karakterizirana je alternativna transkripcija sa varijantama prerade.[6]
Reference[uredi | uredi izvor]
- ^ a b c GRCh38: Ensembl release 89: ENSG00000162607 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000028560 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Puente XS, Sanchez LM, Overall CM, Lopez-Otin C (Jul 2003). "Human and mouse proteases: a comparative genomic approach". Nat Rev Genet. 4 (7): 544–58. doi:10.1038/nrg1111. PMID 12838346. S2CID 2856065.
- ^ a b "Entrez Gene: USP1 ubiquitin specific peptidase 1".
- ^ "UniProt, O94782" (jezik: engleski). Pristupljeno 1. 12. 2021.
Dopunska literatura[uredi | uredi izvor]
- D'Andrea A, Pellman D (1999). "Deubiquitinating enzymes: a new class of biological regulators". Crit. Rev. Biochem. Mol. Biol. 33 (5): 337–52. doi:10.1080/10409239891204251. PMID 9827704.
- Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
- Fujiwara T, Saito A, Suzuki M, et al. (1999). "Identification and chromosomal assignment of USP1, a novel gene encoding a human ubiquitin-specific protease". Genomics. 54 (1): 155–8. doi:10.1006/geno.1998.5554. PMID 9806842.
- Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
- Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
- Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
- Nijman SM, Huang TT, Dirac AM, et al. (2005). "The deubiquitinating enzyme USP1 regulates the Fanconi anemia pathway". Mol. Cell. 17 (3): 331–9. doi:10.1016/j.molcel.2005.01.008. hdl:1874/17796. PMID 15694335.
- Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.