ZZZ3

ZZZ3
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2FC7, 2YUM
Identifikatori
Aliasi	ZZZ3
Vanjski ID-jevi	MGI: 1920453 HomoloGene: 9182 GeneCards: ZZZ3
Lokacija gena (čovjek)
Hrom.	Hromosom 1 (čovjek)
Kraj	77,683,419 bp
Lokacija gena (miš)
Hrom.	Hromosom 3 (miš)
Kraj	152,168,463 bp
Ontologija gena
Molekularna funkcija	• vezivanje sa DNK; • vezivanje iona cinka; • GO:0001948, GO:0016582 vezivanje za proteine; • vezivanje iona metala; • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific;
Ćelijska komponenta	• Jedarce; • jedro;
Biološki proces	• GO:0009373 regulation of transcription, DNA-templated; • transcription, DNA-templated; • GO:0044324, GO:0003256, GO:1901213, GO:0046019, GO:0046020, GO:1900094, GO:0061216, GO:0060994, GO:1902064, GO:0003258, GO:0072212 regulation of transcription by RNA polymerase II;
	Izvori:Amigo / QuickGO
Ortolozi
	26009
	108946
	ENSG00000036549
	ENSMUSG00000039068
	Q8IYH5
	Q6KAQ7
	NM_001308237; NM_015534
	NM_001080755; NM_001287139; NM_198416; NM_001346655; NM_001355673
NP_001295166; NP_056349; NP_001363075; NP_001363076; NP_001363077;
	NP_001363078; NP_001363080; NP_001363082; NP_001363083; NP_001363084; NP_001363085
	NP_001074224; NP_001274068; NP_001333584; NP_001342602
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Protein 3 sa ZZ-tipim cinkovog prsta jest protein koji je kod ljudi kodiran genom ZZZ3 sa hromosoma 1.^[5]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 903 aminokiseline, a molekulska težina 102.023 Da.^[6]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MAASRSTRVT	RSTVGLNGLD	ESFCGRTLRN	RSIAHPEEIS	SNSQVRSRSP
KKRPEPVPIQ	KGNNNGRTTD	LKQQSTRESW	VSPRKRGLSS	SEKDNIERQA
IENCERRQTE	PVSPVLKRIK	RCLRSEAPNS	SEEDSPIKSD	KESVEQRSTV
VDNDADFQGT	KRACRCLILD	DCEKREIKKV	NVSEEGPLNS	AVVEEITGYL
AVNGVDDSDS	AVINCDDCQP	DGNTKQNSIG	SYVLQEKSVA	ENGDTDTQTS
MFLDSRKEDS	YIDHKVPCTD	SQVQVKLEDH	KIVTACLPVE	HVNQLTTEPA
TGPFSETQSS	LRDSEEEVDV	VGDSSASKEQ	CKENTNNELD	TSLESMPASG
EPEPSPVLDC	VSAQMMSLSE	PQEHRYTLRT	SPRRAAPTRG	SPTKNSSPYR
ENGQFEENNL	SPNETNATVS	DNVSQSPTNP	GEISQNEKGI	CCDSQNNGSE
GVSKPPSEAR	LNIGHLPSAK	ESASQHITEE	EDDDPDVYYF	ESDHVALKHN
KDYQRLLQTI	AVLEAQRSQA	VQDLESLGRH	QREALKNPIG	FVEKLQKKAD
IGLPYPQRVV	QLPEIVWDQY	THSLGNFERE	FKNRKRHTRR	VKLVFDKVGL
PARPKSPLDP	KKDGESLSYS	MLPLSDGPEG	SSSRPQMIRG	RLCDDTKPET
FNQLWTVEEQ	KKLEQLLIKY	PPEEVESRRW	QKIADELGNR	TAKQVASRVQ
KYFIKLTKAG	IPVPGRTPNL	YIYSKKSSTS	RRQHPLNKHL	FKPSTFMTSH
EPPVYMDEDD	DRSCFHSHMN	TAVEDASDDE	SIPIMYRNLP	EYKELLQFKK
LKKQKLQQMQ	AESGFVQHVG	FKCDNCGIEP	IQGVRWHCQD	CPPEMSLDFC
DSCSDCLHET	DIHKEDHQLE	PIYRSETFLD	RDYCVSQGTS	YNYLDPNYFP
ANR

Modelni organizmi[uredi | uredi izvor]

Fenotip *Zzz3* nokaut-miševa
Svojstvo	Fenotip
Vijabilnost homozigota	Nenormalnan
Studija recesivne letalnosti	Nenormalnan
Tjeelsna težina	Normalan
Anksioznost otvorenog polja	Normalan
Neurološka peocjena	Normalan
Snaga stiska	Normalan
Test vruće ploče	Normalan
Dismorfologija	Normalan
Indirektna kalorimetrija	Normalan
Test tolerancije glukoze	Normalan
Slušni odgovor moždanog stabla	Normalan
DEXA	Normalan
Radiografija	Normalan
Tjelesna temperatura	Normalan
Morfologija oka	Normalan
Klinička hemija	Normalan
Hematologija	Normalan
Limfociti periferne krvi	Normalan
Mikronukleus test	Normalan
Težina srca	Normalan
Tail epidermis wholemount	Normalan
Histopatologija kože	Normalan
Histopatologija mozga	Normalan
Citrobacter infekcija	Normalan^[7]
Svi testovi I analize iz^[8]^[9]

U proučavanju funkcije ZZZ3 korišteni su modelni organizmi. Uvjetna linija nokaut-miševa, zvana Zzz3^{tm1a(EUCOMM)Wtsi}^[10]^[11] generirana je kao dio programa Međunarodnog konzorcija za nokaut-miševe — projekta mutageneze visoke propusnosti za generiranje i distribuciju životinjskih modela bolesti zainteresiranim naučnicima.^[12]^[13]^[14]

Mužjaci i ženke podvrgnute su standardiziranom fenotipskom pregledu, kako bi se utvrdili efekti delecija.^[8]^[15] Obavljena su 24 testa na mutantnim miševima i uočene su dvije značajne abnormalnosti.^[8] Nijedan homozigotni mutantni embrion nije identificiran tokom gestacije, pa stoga nijedan nije preživio do odbijanja. Preostali testovi su sprovedeni na heterozigot nim mutantnim odraslim miševima i nisu identifikovani dalji abnormalni fenotipovi.^[8]

Također pogledajte[uredi | uredi izvor]

ZZ cinkov prst

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000036549 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000039068 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Entrez Gene: zinc finger, ZZ-type containing 3". Pristupljeno 30. 8. 2011.
^ "UniProt, Q8IYH5" (jezik: eng.). Pristupljeno 26. 11. 2021.CS1 održavanje: nepoznati jezik (link)
^ "Citrobacter infection data for Zzz3". Wellcome Trust Sanger Institute.
^ ^a ^b ^c ^d Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
^ Mouse Resources Portal, Wellcome Trust Sanger Institute.
^ "International Knockout Mouse Consortium". Arhivirano s originala, 3. 4. 2012. Pristupljeno 26. 11. 2021.
^ "Mouse Genome Informatics".
^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
^ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
^ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Dopunska literatura[uredi | uredi izvor]

Guelman S, Suganuma T, Florens L, Swanson SK, Kiesecker CL, Kusch T, Anderson S, Yates JR, Washburn MP, Abmayr SM, Workman JL (Feb 2006). "Host cell factor and an uncharacterized SANT domain protein are stable components of ATAC, a novel dAda2A/dGcn5-containing histone acetyltransferase complex in Drosophila". Molecular and Cellular Biology. 26 (3): 871–82. doi:10.1128/MCB.26.3.871-882.2006. PMC 1347012. PMID 16428443.
Ravasi T, Suzuki H, Cannistraci CV, Katayama S, Bajic VB, Tan K, Akalin A, Schmeier S, Kanamori-Katayama M, Bertin N, Carninci P, Daub CO, Forrest AR, Gough J, Grimmond S, Han JH, Hashimoto T, Hide W, Hofmann O, Kamburov A, Kaur M, Kawaji H, Kubosaki A, Lassmann T, van Nimwegen E, MacPherson CR, Ogawa C, Radovanovic A, Schwartz A, Teasdale RD, Tegnér J, Lenhard B, Teichmann SA, Arakawa T, Ninomiya N, Murakami K, Tagami M, Fukuda S, Imamura K, Kai C, Ishihara R, Kitazume Y, Kawai J, Hume DA, Ideker T, Hayashizaki Y (Mar 2010). "An atlas of combinatorial transcriptional regulation in mouse and man". Cell. 140 (5): 744–52. doi:10.1016/j.cell.2010.01.044. PMC 2836267. PMID 20211142.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000036549 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000039068 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] "Entrez Gene: zinc finger, ZZ-type containing 3". Pristupljeno 30. 8. 2011.

[UniProt-6] "UniProt, Q8IYH5" (jezik: eng.). Pristupljeno 26. 11. 2021.CS1 održavanje: nepoznati jezik (link)

[''Citrobacter''_infection-7] "Citrobacter infection data for Zzz3". Wellcome Trust Sanger Institute.

[mgp_reference-8] Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.

[9] Mouse Resources Portal, Wellcome Trust Sanger Institute.

[allele_ref-10] "International Knockout Mouse Consortium". Arhivirano s originala, 3. 4. 2012. Pristupljeno 26. 11. 2021.

[mgi_allele_ref-11] "Mouse Genome Informatics".

[pmid21677750-12] Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.

[mouse_library-13] Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.

[mouse_for_all_reasons-14] Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.

[pmid21722353-15] van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

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