EVI5
Proteinski homolog ekotropskog virusnog integracijskog mjesta 5 jest protein koji je kod ljudi kodiran genom EVI5 sa hromosoma 1.[5][6]
Amiokiselininska sekvenca[uredi | uredi izvor]
Dužina polipeptidnog lanca je 810 aminokiselina, a molekulska težina 92.949 Da.[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MVTNKMTAAF | RNPSGKQVAT | DKVAEKLSST | LSWVKNTVSH | TVSQMASQVA | ||||
| SPSTSLHTTS | SSTTLSTPAL | SPSSPSQLSP | DDLELLAKLE | EQNRLLETDS | ||||
| KSLRSVNGSR | RNSGSSLVSS | SSASSNLSHL | EEDSWILWGR | IVNEWEDVRK | ||||
| KKEKQVKELV | HKGIPHHFRA | IVWQLLCSAQ | SMPIKDQYSE | LLKMTSPCEK | ||||
| LIRRDIARTY | PEHNFFKEKD | SLGQEVLFNV | MKAYSLVDRE | VGYCQGSAFI | ||||
| VGLLLMQMPE | EEAFCVFVKL | MQDYRLRELF | KPSMAELGLC | MYQFECMIQE | ||||
| HLPELFVHFQ | SQSFHTSMYA | SSWFLTIFLT | TFPLPIATRI | FDIFMSEGLE | ||||
| IVFRVGLALL | QMNQAELMQL | DMEGMLQHFQ | KVIPHQFDGV | PDKLIQAAYQ | ||||
| VKYNSKKMKK | LEKEYTTIKT | KEMEEQVEIK | RLRTENRLLK | QRIETLEKHK | ||||
| CSSNYNEDFV | LQLEKELVQA | RLSEAESQCA | LKEMQDKVLD | IEKRNNSLPD | ||||
| ENNIARLQEE | LIAVKLREAE | AIMGLKELRQ | QVKDLEEHWQ | RHLARTTGRW | ||||
| KDPPKKNAMN | ELQDELMTIR | LREAETQAEI | REIKQRMMEM | ETQNQINSNH | ||||
| LRRAEQEVIS | LQEKVQYLSA | QNKGLLTQLS | EAKRKQAEIE | CKNKEEVMAV | ||||
| RLREADSIAA | VAELRQHIAE | LEIQKEEGKL | QGQLNKSDSN | QYIGELKDQI | ||||
| AELNHELRCL | KGQRGFSGQP | PFDGIHIVNH | LIGDDESFHS | SDEDFIDNSL | ||||
| QETGVGFPLH | GKSGSMSLDP | AVADGSESET | EDSVLETRES | NQVVQKERPP | ||||
| RRRESYSTTV |
Modelni organizmi[uredi | uredi izvor]
U proučavanju funkcije EVI5 korišteni su modelni organizmi. Uslovna nokaut-mišja linija pod nazivom Evi5tm1a(KOMP)Wtsi generirana je u Institutu Wellcome Trust Sanger.[8] Mužjaci i ženke podvrgnuti su standardizovanom fenotipskom pregledu[9] za ordeđivanje efekata delecija.[10][11][12][13] Urađeni su dodatni pregledi: Dubinska imunološka fenotipizacija[14]
| Svojstvo | Fenotip |
|---|---|
| Svi podaci raspoloživi.[9][14] | |
| Leukociti periferne krvi 6 sedmica | Normalan |
| Insulin | Normalan |
| Hematologija 6 sedmica | Normalan |
| Vijabilnost homozigota na P14 | Normalan |
| Plodnost homozigota | Normalan |
| Tjelesna težina | Normalan |
| Neurološka procjena | Normalan |
| Snaga stiska | Normalan |
| Dismorfologija | Normalan |
| Indirektna kalorimetrija | Normalan |
| Test tolerancije glukoze | Normalan |
| Slušni odgovor moždanog stabla | Normalan |
| DEXA | Normalan |
| Radiografija | Normalan |
| Morfologija oka | Normalan |
| Klinička hemija | Normalan |
| Hematologija 16 sedmica | Normalan |
| Leukociti periferne krvi 16 sedmica | Normalan |
| Težina srca | Normalan |
| Salmonella infekcija | Normalan |
| Funkcija citotoksičnih T-ćelija | Normalan |
| Imunofenotipirzacija slezene | Normalan |
| Imunofenotipirzacija mezenternog limfnog čvora | Normalan |
| Imunofenotipirzacija koštane srži | Normalan |
| Kompoziocija epidermne imunosti | Normalan |
Reference[uredi | uredi izvor]
- ^ a b c GRCh38: Ensembl release 89: ENSG00000067208 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000011831 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Roberts T, Chernova O, Cowell JK (Jul 1998). "NB4S, a member of the TBC1 domain family of genes, is truncated as a result of a constitutional t(1;10)(p22;q21) chromosome translocation in a patient with stage 4S neuroblastoma". Human Molecular Genetics. 7 (7): 1169–78. doi:10.1093/hmg/7.7.1169. PMID 9618176.
- ^ "Entrez Gene: EVI5 ecotropic viral integration site 5".
- ^ "UniProt, O60447" (jezik: engleski). Pristupljeno 25. 11. 2021.
- ^ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
- ^ a b "International Mouse Phenotyping Consortium".
- ^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- ^ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ^ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
- ^ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
- ^ a b "Infection and Immunity Immunophenotyping (3i) Consortium".[mrtav link]
Dopunska literatura[uredi | uredi izvor]
- Dabbeekeh JT, Faitar SL, Dufresne CP, Cowell JK (Apr 2007). "The EVI5 TBC domain provides the GTPase-activating protein motif for RAB11". Oncogene. 26 (19): 2804–8. doi:10.1038/sj.onc.1210081. PMID 17099728.
- Westlake CJ, Junutula JR, Simon GC, Pilli M, Prekeris R, Scheller RH, Jackson PK, Eldridge AG (Jan 2007). "Identification of Rab11 as a small GTPase binding protein for the Evi5 oncogene". Proceedings of the National Academy of Sciences of the United States of America. 104 (4): 1236–41. Bibcode:2007PNAS..104.1236W. doi:10.1073/pnas.0610500104. PMC 1773056. PMID 17229837.
- Faitar SL, Sossey-Alaoui K, Ranalli TA, Cowell JK (Jul 2006). "EVI5 protein associates with the INCENP-aurora B kinase-survivin chromosomal passenger complex and is involved in the completion of cytokinesis". Experimental Cell Research. 312 (12): 2325–35. doi:10.1016/j.yexcr.2006.03.032. PMID 16764853.
- Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (Apr 2006). "Phosphoproteome analysis of the human mitotic spindle". Proceedings of the National Academy of Sciences of the United States of America. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
- Eldridge AG, Loktev AV, Hansen DV, Verschuren EW, Reimann JD, Jackson PK (Jan 2006). "The evi5 oncogene regulates cyclin accumulation by stabilizing the anaphase-promoting complex inhibitor emi1". Cell. 124 (2): 367–80. doi:10.1016/j.cell.2005.10.038. PMID 16439210. S2CID 15578836.
- Faitar SL, Dabbeekeh JT, Ranalli TA, Cowell JK (Nov 2005). "EVI5 is a novel centrosomal protein that binds to alpha- and gamma-tubulin". Genomics. 86 (5): 594–605. doi:10.1016/j.ygeno.2005.06.002. PMID 16033705.
- Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, Li Y, Xu C, Fang R, Guegler K, Rao MS, Mandalam R, Lebkowski J, Stanton LW (Jun 2004). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nature Biotechnology. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197. S2CID 27764390.