S Wikipedije, slobodne enciklopedije
Podjedinica 12 integratorskog kompleksa (Int12) znana i kao PHD-prstni protein 22 (PHF22) jest protein koji je kod ljudi kodiran genom INTS12 sa hromosoma 4 .[4]
Dužina polipeptidnog lanca je 462 aminokiseline , а molekulska težina 48.808 Da .[5]
10 20 30 40 50 MAATVNLELD PIFLKALGFL HSKSKDSAEK LKALLDESLA RGIDSSYRPS QKDVEPPKIS STKNISIKQE PKISSSLPSG NNNGKVLTTE KVKKEAEKRP ADKMKSDITE GVDIPKKPRL EKPETQSSPI TVQSSKDLPM ADLSSFEETS ADDFAMEMGL ACVVCRQMMV ASGNQLVECQ ECHNLYHRDC HKPQVTDKEA NDPRLVWYCA RCTRQMKRMA QKTQKPPQKP APAVVSVTPA VKDPLVKKPE TKLKQETTFL AFKRTEVKTS TVISGNSSSA SVSSSVTSGL TGWAAFAAKT SSAGPSTAKL SSTTQNNTGK PATSSANQKP VGLTGLATSS KGGIGSKIGS NNSTTPTVPL KPPPPLTLGK TGLSRSVSCD NVSKVGLPSP SSLVPGSSSQ LSGNGNSGTS GPSGSTTSKT TSESSSSPSA SLKGPTSQES QLNAMKRLQM VKKKAAQKKL KK
INTS12 jest podjedinica integratorskog kompleksa , pridružena C-terminalnom domenu RNK-polimeraze II velike podjedinice (POLR2A ) i posreduje 3' preradu malih jedarnih RNK U1 (RNU1 ) i U2 (RNU2 )[4] [6]
U istraživanji posljedica delecija gena INT12 upotrebljeni su i modelni organizmi nokaut-miševa linije Ints12
Modelni organizmi bili su iz linije nokaut-miševa zvane Ints12 , generirani po programu Međunarodnog konzorcija za nokaut-miševe — visokopropusnog projekta za mutagenezu , generiranje životinjskih modela, na Institutu Wellcome Trust Sanger , zainteresiranim naučnicima[11] [12] [13]
Kalsi G, Kuo PH, Aliev F, et al. (2010). "A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts" . Hum. Mol. Genet . 19 (12): 2497–506. doi :10.1093/hmg/ddq112 . PMC 2876884 PMID 20332099 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 .
^ a b c GRCm38: Ensembl release 89: ENSMUSG00000028016 - Ensembl , maj 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ a b "Entrez Gene: integrator complex subunit 12" . Pristupljeno 30. 8. 2011 .^ "UniProt, Q96CB8" (jezik: engleski). Pristupljeno 28. 10. 2021 .^ Baillat D, Hakimi MA, et al. (2005). "Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II" . Cell . 123 (2): 265–76. doi :10.1016/j.cell.2005.08.019 PMID 16239144 . S2CID 18069461 . ^ "Salmonella infection data for Mtrf1l" . Wellcome Trust Sanger Institute.^ "Citrobacter infection data for Mtrf1l" . Wellcome Trust Sanger Institute.^ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica . 88 (S248). doi :10.1111/j.1755-3768.2010.4142.x . S2CID 85911512 . ^ Mouse Resources Portal , Wellcome Trust Sanger Institute.^ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function" . Nature 474 (7351): 337–342. doi :10.1038/nature10163 . PMC 3572410 PMID 21677750 . ^ Dolgin E (juni 2011). "Mouse library set to be knockout" . Nature 474 (7351): 262–3. doi :10.1038/474262a PMID 21677718 . ^ Collins FS, Rossant J, Wurst W (januar 2007). "A mouse for all reasons" . Cell . 128 (1): 9–13. doi :10.1016/j.cell.2006.12.018 PMID 17218247 . S2CID 18872015 . 
