NOL12

NOL12
Identifikatori
Aliasi	NOL12
Vanjski ID-jevi	MGI: 2146285 HomoloGene: 32577 GeneCards: NOL12
Lokacija gena (čovjek)
Hrom.	Hromosom 22 (čovjek)
Kraj	37,693,476 bp
Lokacija gena (miš)
Hrom.	Hromosom 15 (miš)
Kraj	78,827,838 bp
Obrazac RNK ekspresije
	; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• rRNA binding; • GO:0001948, GO:0016582 vezivanje za proteine; • vezivanje sa RNK; • single-stranded DNA binding;
Ćelijska komponenta	• Jedarce; • jedro;
Biološki proces	• rRNA processing; • GO:0007576 nucleolus organization; • positive regulation of cell population proliferation; • positive regulation of cell growth; • negative regulation of apoptotic process;
	Izvori:Amigo / QuickGO
Ortolozi
	79159
	97961
	ENSG00000273899
	ENSMUSG00000033099
	Q9UGY1
	Q8BG17
	NM_024313
	NM_133800
	NP_077289
	NP_598561
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Jedarčani protein 12 jest protein koji je kod ljudi kodiran genom NOL12 sa hromosoma 22.^[5]^[6]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 213 aminokiseline, a molekulska težina 24.663 Da.^[6]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MGRNKKKKRD	GDDRRPRLVL	SFDEEKRREY	LTGFHKRKVE	RKKAAIEEIK
QRLKEEQRKL	REERHQEYLK	MLAEREEALE	EADELDRLVT	AKTESVQYDH
PNHTVTVTTI	SDLDLSGARL	LGLTPPEGGA	GDRSEEEASS	TEKPTKALPR
KSRDPLLSQR	ISSLTASLHA	HSRKKVKRKH	PRRAQDSKKP	PRAPRTSKAQ
RRRLTGKARH	SGE

Funkcija

Pokazalo se da se ljudskii NOL12 nalazi u jedarcetuu i reguliše jedarčanu strukturu i homeostazu, održavanjem nivoa multifunkcionalnih fibrilarinskih i nukleolinskih proteina. Njegov nedostatak dovodi do aktivacije p53, što rezultira zaustavljanjem ćelijskog ciklusa u njgovoj fazi G2. Nol12 ili hNol12 pokreću p53 indukovano starenje ćelija sugerišući njegovu važnu ulogu u starenju.^[7] Ljudski Nol12 je također potreban za sazrijevanje ribosoma i integritet genoma.^[8]

Porodica Nol12

Ljudski Nol12 pripada porodici proteina poznatih kao ribosomski vezujući proteini RBP-i. Ljudski članovi imaju ekvivalente u različitim vrstama. Njegov homolog u Drosophila melanogaster zove se Viriato, koji je uključen u razvoj očiju i nervnog sistema i bez njega, kod Drosophila melanogaster ne uspijeva razvoj oka. Gubitak "Viriato" također rezultirao je proliferacijom ćelija, kašnjenjem u razvoju i apoptozama.^[9]^[10] hNOL12 homologue in rats is known as Nop25 and is responsible for nucleolar structure and integrity.^[11] Njegov homolog kod kvasca nazvan je Rrp17, za koji je pokazano da ima 5′-3′ egzonukleaznu aktivnost i da je uključen u biogenezu ribosoma.^[12]

Reference

1 2 3 GRCh38: Ensembl release 89: ENSG00000273899 - Ensembl, maj 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000033099 - Ensembl, maj 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, et al. (December 2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proceedings of the National Academy of Sciences of the United States of America. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
1 2 "Entrez Gene: NOL12 nucleolar protein 12".
↑ Pinho M, Macedo JC, Logarinho E, Pereira PS (June 2019). "NOL12 Repression Induces Nucleolar Stress-Driven Cellular Senescence and Is Associated with Normative Aging". Molecular and Cellular Biology. 39 (12). doi:10.1128/MCB.00099-19. PMC 6549468. PMID 30988155.
↑ Scott DD, Trahan C, Zindy PJ, Aguilar LC, Delubac MY, Van Nostrand EL, et al. (December 2017). "Nol12 is a multifunctional RNA binding protein at the nexus of RNA and DNA metabolism". Nucleic Acids Research. 45 (21): 12509–12528. doi:10.1093/nar/gkx963. PMC 5716212. PMID 29069457.
↑ Marinho J, Martins T, Neto M, Casares F, Pereira PS (May 2013). "The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development". Developmental Biology. 377 (1): 154–65. doi:10.1016/j.ydbio.2013.02.003. PMID 23416177.
↑ Marinho J, Casares F, Pereira PS (January 2011). "The Drosophila Nol12 homologue viriato is a dMyc target that regulates nucleolar architecture and is required for dMyc-stimulated cell growth". Development. 138 (2): 349–57. doi:10.1242/dev.054411. PMID 21177347. S2CID 18287789.
↑ Suzuki S, Fujiwara T, Kanno M (July 2007). "Nucleolar protein Nop25 is involved in nucleolar architecture". Biochemical and Biophysical Research Communications. 358 (4): 1114–9. doi:10.1016/j.bbrc.2007.05.069. PMID 17524363.
↑ Oeffinger M, Zenklusen D, Ferguson A, Wei KE, El Hage A, Tollervey D, et al. (December 2009). "Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA". Molecular Cell. 36 (5): 768–81. doi:10.1016/j.molcel.2009.11.011. PMC 2806520. PMID 20005841.

Dopunska literatura

Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (November 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (April 2006). "Phosphoproteome analysis of the human mitotic spindle". Proceedings of the National Academy of Sciences of the United States of America. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, et al. (2005). "A genome annotation-driven approach to cloning the human ORFeome". Genome Biology. 5 (10): R84. doi:10.1186/gb-2004-5-10-r84. PMC 545604. PMID 15461802.
Collins JE, Goward ME, Cole CG, Smink LJ, Huckle EJ, Knowles S, et al. (January 2003). "Reevaluating human gene annotation: a second-generation analysis of chromosome 22". Genome Research. 13 (1): 27–36. doi:10.1101/gr.695703. PMC 430954. PMID 12529303.
Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, et al. (December 1999). "The DNA sequence of human chromosome 22". Nature. 402 (6761): 489–95. Bibcode:1999Natur.402..489D. doi:10.1038/990031. PMID 10591208.

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000273899 - Ensembl, maj 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000033099 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid12477932-5] Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, et al. (December 2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proceedings of the National Academy of Sciences of the United States of America. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.

[entrez-6] 1 2 "Entrez Gene: NOL12 nucleolar protein 12".

[7] Pinho M, Macedo JC, Logarinho E, Pereira PS (June 2019). "NOL12 Repression Induces Nucleolar Stress-Driven Cellular Senescence and Is Associated with Normative Aging". Molecular and Cellular Biology. 39 (12). doi:10.1128/MCB.00099-19. PMC 6549468. PMID 30988155.

[8] Scott DD, Trahan C, Zindy PJ, Aguilar LC, Delubac MY, Van Nostrand EL, et al. (December 2017). "Nol12 is a multifunctional RNA binding protein at the nexus of RNA and DNA metabolism". Nucleic Acids Research. 45 (21): 12509–12528. doi:10.1093/nar/gkx963. PMC 5716212. PMID 29069457.

[9] Marinho J, Martins T, Neto M, Casares F, Pereira PS (May 2013). "The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development". Developmental Biology. 377 (1): 154–65. doi:10.1016/j.ydbio.2013.02.003. PMID 23416177.

[10] Marinho J, Casares F, Pereira PS (January 2011). "The Drosophila Nol12 homologue viriato is a dMyc target that regulates nucleolar architecture and is required for dMyc-stimulated cell growth". Development. 138 (2): 349–57. doi:10.1242/dev.054411. PMID 21177347. S2CID 18287789.

[11] Suzuki S, Fujiwara T, Kanno M (July 2007). "Nucleolar protein Nop25 is involved in nucleolar architecture". Biochemical and Biophysical Research Communications. 358 (4): 1114–9. doi:10.1016/j.bbrc.2007.05.069. PMID 17524363.

[12] Oeffinger M, Zenklusen D, Ferguson A, Wei KE, El Hage A, Tollervey D, et al. (December 2009). "Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA". Molecular Cell. 36 (5): 768–81. doi:10.1016/j.molcel.2009.11.011. PMC 2806520. PMID 20005841.

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