S Wikipedije, slobodne enciklopedije
TRAF-tip domena cinkovog prsta sa proteinom 1 je protein koji je kod ljudi kodiran genom TRAFD1 .[5] [6]
Dužina polipeptidnog lanca je 582 aminokiselina , a molekulska težina 64.841 Da .[7]
10 20 30 40 50 MAEFLDDQET RLCDNCKKEI PVFNFTIHEI HCQRNIGMCP TCKEPFPKSD METHMAAEHC QVTCKCNKKL EKRLLKKHEE TECPLRLAVC QHCDLELSIL KLKEHEDYCG ARTELCGNCG RNVLVKDLKT HPEVCGREGE EKRNEVAIPP NAYDESWGQD GIWIASQLLR QIEALDPPMR LPRRPLRAFE SDVFHNRTTN QRNITAQVSI QNNLFEEQER QERNRGQQPP KEGGEESANL DFMLALSLQN EGQASSVAEQ DFWRAVCEAD QSHGGPRSLS DIKGAADEIM LPCEFCEELY PEELLIDHQT SCNPSRALPS LNTGSSSPRG VEEPDVIFQN FLQQAASNQL DSLMGLSNSH PVEESIIIPC EFCGVQLEEE VLFHHQDQCD QRPATATNHV TEGIPRLDSQ PQETSPELPR RRVRHQGDLS SGYLDDTKQE TANGPTSCLP PSRPINNMTA TYNQLSRSTS GPRPGCQPSS PCVPKLSNSD SQDIQGRNRD SQNGAIAPGH VSVIRPPQNL YPENIVPSFS PGPSGRYGAS GRSEGGRNSR VTPAAANYRS RTAKAKPSKQ QGAGDAEEEE EE
U proučavanju PNPO funkcije, korišteni su modelni organizmi . Uvjetna linija nokaut-miševa , zvana Pnpotm1a (KOMP) Wtsi , [12] [13] Međunarosnog konzorcija za miševe – visokopropusnog projekta mutageneze za generiranje i distribuciju životinjskih modela bolesti zainteresiranim naučnicima.[14] [15] [16]
Muške i ženske životinje prolazile su standardizirani fenotipski skrining , kako bi se utvrdili efekti delecije.[10] [17] mutantnim miševima, uočene su dvije značajne abnormalnosti.[10] gestacije , nije utvrđen homozigotni mutantni embrion , pa tako nijedan nije preživio do odvikavanja. Preostali testovi su izvedeni na mutiranim heterozigotnim odraslim miševima; nisu opažene nikakve dodatne značajne abnormalnosti kod ovih životinja.[10]
^ a b c GRCh38: Ensembl release 89: ENSG00000135148 - Ensembl , maj 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000042726 - Ensembl , maj 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA (Dec 2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc Natl Acad Sci U S A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 . PMC 139241 PMID 12477932 . ^ "Entrez Gene: TRAFD1 TRAF-type zinc finger domain containing 1" .^ "UniProt, O14545" . Pristupljeno 14. 8. 2021 .^ "Salmonella infection data for Pnpo" . Wellcome Trust Sanger Institute.^ "Citrobacter infection data for Pnpo" . Wellcome Trust Sanger Institute.^ a b c d Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica . 88 : 925–7. doi :10.1111/j.1755-3768.2010.4142.x . S2CID 85911512 . ^ Mouse Resources Portal , Wellcome Trust Sanger Institute.^ "International Knockout Mouse Consortium" . Arhivirano s originala , 3. 4. 2012. Pristupljeno 14. 8. 2021 .^ "Mouse Genome Informatics" .^ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function" . Nature 474 (7351): 337–342. doi :10.1038/nature10163 . PMC 3572410 PMID 21677750 . ^ Dolgin E (2011). "Mouse library set to be knockout" . Nature . 474 (7351): 262–3. doi :10.1038/474262a PMID 21677718 . ^ Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell . 128 (1): 9–13. doi :10.1016/j.cell.2006.12.018 . PMID 17218247 . S2CID 18872015 . ^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism" . Genome Biol . 12 (6): 224. doi :10.1186/gb-2011-12-6-224 . PMC 3218837 PMID 21722353 .
Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks" . Cell . 127 (3): 635–48. doi :10.1016/j.cell.2006.09.026 PMID 17081983 . S2CID 7827573 . Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization" . Nat. Biotechnol . 24 (10): 1285–92. doi :10.1038/nbt1240 . PMID 16964243 . S2CID 14294292 . Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle" . Proc. Natl. Acad. Sci. U.S.A . 103 (14): 5391–6. Bibcode :2006PNAS..103.5391N . doi :10.1073/pnas.0507066103 . PMC 1459365 PMID 16565220 . Mashima R, Saeki K, Aki D, et al. (2006). "FLN29, a novel interferon- and LPS-inducible gene acting as a negative regulator of toll-like receptor signaling" . J. Biol. Chem . 280 (50): 41289–97. doi :10.1074/jbc.M508221200 PMID 16221674 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins" . Proc. Natl. Acad. Sci. U.S.A . 101 (33): 12130–5. Bibcode :2004PNAS..10112130B . doi :10.1073/pnas.0404720101 . PMC 514446 PMID 15302935 .
