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Protein 13D pridružen vakuolskom sortiranju
jest protein koji je kod ljudi kodiran genom VPS13D sa hromosoma 1 .[5]
Dužina polipeptidnog lanca je 4.388 aminokiselina , a molekulska težina 491.916 Da .[6]
10 20 30 40 50 MLEGLVAWVL NTYLGKYVNN LNTDQLSVAL LKGAVELENL PLKKDALKEL ELPFEVKAGF IGKVTLQIPF YRPHVDPWVI SISSLHLIGA PEKIQDFNDE KEKLLERERK KALLQALEEK WKNDRQQKGE SYWYSVTASV VTRIVENIEL KIQDVHLRFE DGVTNPSHPF AFGICIKNVS MQNAVNEPVQ KLMRKKQLDV AEFSIYWDVD CTLLGDLPQM ELQEAMARSM ESRSHHYVLE PVFASALLKR NCSKKPLRSR HSPRIDCDIQ LETIPLKLSQ LQYRQIMEFL KELERKERQV KFRRWKPKVA ISKNCREWWY FALNANLYEI REQRKRCTWD FMLHRARDAV SYTDKYFNKL KGGLLSTDDK EEMCRIEEEQ SFEELKILRE LVHDRFHKQE ELAESLREPQ FDSPGACPGA PEPGGGSGML QYLQSWFPGW GGWYGQQTPE GNVVEGLSAE QQEQWIPEEI LGTEEFFDPT ADASCMNTYT KRDHVFAKLN LQLQRGTVTL LHKEQGTPQM NESAFMQLEF SDVKLLAESL PRRNSSLLSV RLGGLFLRDL ATEGTMFPLL VFPNPQKEVG RVSQSFGLQT TSADRSDHYP AADPDGPVFE MLYERNPAHS HFERRLNVST RPLNIIYNPQ AIKKVADFFY KGKVHTSGFG YQSELELRVA EAARRQYNKL KMQTKAEIRQ TLDRLLVGDF IEESKRWTVR LDISAPQVIF PDDFKFKNPV LVVVDLGRML LTNTQDNSRR KSRDGSASEE TQFSDDEYKT PLATPPNTPP PESSSSNGEK TPPFSGVEFS EEQLQAHLMS TKMYERYSLS FMDLQIMVGR VKDNWKHVQD IDVGPTHVVE KFNVHLQLER RLIYTSDPKY PGAVLSGNLP DLKIHINEDK ISALKNCFAL LTTPEMKTSD TQIKEKIFPQ EEQRGSLQDS VMNLTQSIVL LEQHTREVLV ESQLLLAEFK VNCMQLGVES NGRYISVLKV FGTNAHFVKR PYDAEVSLTV HGLLLVDTMQ TYGADFDLLM ASHKNLSFDI PTGSLRDSRA QSPVSGPNVA HLTDGATLND RSATSVSLDK ILTKEQESLI KLEYQFVSSE CPSMNLDSTL QVISLQVNNL DIILNPETIV ELIGFLQKSF PKEKDDLSPQ PLMTDFERSF REQGTYQSTY EQNTEVAVEI HRLNLLLLRT VGMANREKYG RKIATASIGG TKVNVSMGST FDMNGSLGCL QLMDLTQDNV KNQYVVSIGN SVGYENIISD IGYFESVFVR MEDAALTEAL SFTFVERSKQ ECFLNLKMAS LHYNHSAKFL KELTLSMDEL EENFRGMLKS AATKVTTVLA TKTAEYSEMV SLFETPRKTR EPFILEENEI YGFDLASSHL DTVKLILNIN IESPVVSIPR KPGSPELLVG HLGQIFIQNF VAGDDESRSD RLQVEIKDIK LYSLNCTQLA GREAVGSEGS RMFCPPSGSG SANSQEEAHF TRHDFFESLH RGQAFHILNN TTIQFKLEKI PIERESELTF SLSPDDLGTS SIMKIEGKFV NPVQVVLAKH VYEQVLQTLD NLVYSEDLNK YPASATSSPC PDSPLPPLST CGESSVERKE NGLFSHSSLS NTSQKSLSVK EVKSFTQIQA TFCISELQVQ LSGDLTLGAQ GLVSLKFQDF EVEFSKDHPQ TLSIQIALHS LLMEDLLEKN PDSKYKNLMV SRGAPKPSSL AQKEYLSQSC PSVSNVEYPD MPRSLPSHME EAPNVFQLYQ RPTSASRKKQ KEVQDKDYPL TPPPSPTVDE PKILVGKSKF DDSLVHINIF LVDKKHPEFS SSYNRVNRSI DVDFNCLDVL ITLQTWVVIL DFFGIGSTAD NHAMRLPPEG ILHNVKLEPH ASMESGLQDP VNTKLDLKVH SLSLVLNKTT SELAKANVSK LVAHLEMIEG DLALQGSIGS LSLSDLTCHG EFYRERFTTS GEEALIFQTF KYGRPDPLLR REHDIRVSLR MASVQYVHTQ RFQAEVVAFI QHFTQLQDVL GRQRAAIEGQ TVRDQAQRCS RVLLDIEAGA PVLLIPESSR SNNLIVANLG KLKVKNKFLF AGFPGTFSLQ DKESVPSASP TGIPKHSLRK TTSTEEPRGT HSQGQFTMPL AGMSLGSLKS EFVPSTSTKQ QGPQPTLSVG QESSSPEDHV CLLDCVVVDL QDMDIFAAER HPREYSKAPE DSSGDLIFPS YFVRQTGGSL LTEPCRLKLQ VERNLDKEIS HTVPDISIHG NLSSVHCSLD LYKYKLIRGL LENNLGEPIE EFMRPYDLQD PRIHTVLSGE VYTCMCFLID MVNVSLELKD PKRKEGAGSL ARFDFKKCKL LYESFSNQTK SINLVSHSMM AFDTRYAGQK TSPGMTNVFS CIFQPAKNSS TTQGSIQIEL HFRSTKDSSC FTVVLNNLRV FLIFDWLLLV HDFLHTPSDI KKQNHVTPSR HRNSSSESAI VPKTVKSGVV TKRSSLPVSN ERHLEVKVNV TGTEFVVIED VSCFDTNAII LKGTTVLTYK PRFVDRPFSG SLFGIEVFSC RLGNEHDTAL SIVDPVQIQM ELVGNSSYQN SSGLMDAFNS EDFPPVLEIQ LQALDIRLSY NDVQLFLAIA KSIPEQANAA VPDSVALESD SVGTYLPGAS RVGEEIREGT RHTLDPVLEL QLARLQELGF SMDDCRKALL ACQGQLKKAA SWLFKNAEPL KSLSLASTSR DSPGAVAAPL ISGVEIKAES VCICFIDDCM DCDVPLAELT FSRLNFLQRV RTSPEGYAHF TLSGDYYNRA LSGWEPFIEP WPCSVSWQQQ AASRLHPPRL KLEAKAKPRL DINITSVLID QYVSTKESWM ADYCKDDKDI ESAKSEDWMG SSVDPPCFGQ SLPLVYLRTR STASLTNLEH QIYARAEVKT PKRRQPFVPF ALRNHTGCTL WFATLTTTPT RAALSHSGSP GVVPEGNGTF LDDTHNVSEW REVLTGEEIP FEFEARGKLR HRHTHDLRIH QLQVRVNGWE QVSPVSVDKV GTFFRYAAPD KNSSSSTIGS PSSRTNIIHP QVYFSSLPPV RVVFAVTMEG SARKVITVRS ALIVRNRLET PMELRLDSPS APDKPVVLPA IMPGDSFAVP LHLTSWRLQA RPKGLGVFFC KAPIHWTNVV KTAEISSSKR ECHSMDTEKS RFFRFCVAIK KENYPDYMPS NIFSDSAKQI FRQPGHTIYL LPTVVICNLL PCELDFYVKG MPINGTLKPG KEAALHTADT SQNIELGVSL ENFPLCKELL IPPGTQNYMV RMRLYDVNRR QLNLTIRIVC RAEGSLKIFI SAPYWLINKT GLPLIFRQDN AKTDAAGQFE EHELARSLSP LLFCYADKEQ PNLCTMRIGR GIHPEGMPGW CQGFSLDGGS GVRALKVIQQ GNRPGLIYNI GIDVKKGRGR YIDTCMVIFA PRYLLDNKSS HKLAFAQREF ARGQGTANPE GYISTLPGSS VVFHWPRNDY DQLLCVRLMD VPNCIWSGGF EVNKNNSFHI NMRDTLGKCF FLRVEITLRG ATYRISFSDT DQLPPPFRID NFSKVPVVFT QHGVAEPRLR TEVKPMTSLD YAWDEPTLPP FITLTVKGAG SSEINCNMND FQDNRQLYYE NFIYIAATYT FSGLQEGTGR PVASNKAITC AELVLDVSPK TQRVILKKKE PGKRSQLWRM TGTGMLAHEG SSVPHNPNKP SAARSTEGSA ILDIAGLAAV TDNRYEPLML RKPDRRRSTT QTWSFREGKL TCGLHGLVVQ AKGGLSGLFD GAEVVLGPDT SMELLGPVPP EQQFINQKMR PGSGMLSIRV IPDGPTRALQ ITDFCHRKSS RSYEVDELPV TEQELQKLKN PDTEQELEVL VRLEGGIGLS LINKVPEELV FASLTGINVH YTQLATSHML ELSIQDVQVD NQLIGTTQPF MLYVTPLSNE NEVIETGPAV QVNAVKFPSK SALTNIYKHL MITAQRFTVQ IEEKLLLKLL SFFGYDQAES EVEKYDENLH EKTAEQGGTP IRYYFENLKI SIPQIKLSVF TSNKLPLDLK ALKSTLGFPL IRFEDAVINL DPFTRVHPYE TKEFIINDIL KHFQEELLSQ AARILGSVDF LGNPMGLLND VSEGVTGLIK YGNVGGLIRN VTHGVSNSAA KFAGTLSDGL GKTMDNRHQS EREYIRYHAA TSGEHLVAGI HGLAHGIIGG LTSVITSTVE GVKTEGGVSG FISGLGKGLV GTVTKPVAGA LDFASETAQA VRDTATLSGP RTQAQRVRKP RCCTGPQGLL PRYSESQAEG QEQLFKLTDN IQDEFFIAVE NIDSYCVLIS SKAVYFLKSG DYVDREAIFL EVKYDDLYHC LVSKDHGKVY VQVTKKAVST SSGVSIPGPS HQKPMVHVKS EVLAVKLSQE INYAKSLYYE QQLMLRLSEN REQLELDS
Ovaj gen kodira protein koji pripada porodici gena vakuolskih -sortirajućih proteina-13. U kvascima , vakuolno-protein-sortirajući-13 proteini uključeni su u promet membranskih proteina između trans-Golgi jeve mreže i prevakuolskog odjeljka.
Iako za ovaj gen može postojati nekoliko varijanti transkripta , do danas su opisane pune prirode samo dvije. Ova dva predstavljaju glavne varijante ovog gena i kodiraju različite izoforme .[5]
Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem . 236 (1): 107–13. doi :10.1006/abio.1996.0138 . PMID 8619474 . Yu W, Andersson B, Worley KC, et al. (1997). "Large-Scale Concatenation cDNA Sequencing" . Genome Res . 7 (4): 353–8. doi :10.1101/gr.7.4.353 . PMC 139146 PMID 9110174 . Seki N, Ohira M, Nagase T, et al. (1998). "Characterization of cDNA clones in size-fractionated cDNA libraries from human brain" . DNA Res . 4 (5): 345–9. doi :10.1093/dnares/4.5.345 PMID 9455484 . Nakayama M, Kikuno R, Ohara O (2003). "Protein–Protein Interactions Between Large Proteins: Two-Hybrid Screening Using a Functionally Classified Library Composed of Long cDNAs" . Genome Res . 12 (11): 1773–84. doi :10.1101/gr.406902 . PMC 187542 PMID 12421765 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5. doi :10.1038/ng1285 PMID 14702039 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Velayos-Baeza A, Vettori A, Copley RR, et al. (2005). "Analysis of the human VPS13 gene family". Genomics . 84 (3): 536–49. doi :10.1016/j.ygeno.2004.04.012 . PMID 15498460 . Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1" . Nature . 441 (7091): 315–21. Bibcode :2006Natur.441..315G . doi :10.1038/nature04727 PMID 16710414 . 
