VPS72

VPS72
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	5FUG
Identifikatori
Aliasi	VPS72
Vanjski ID-jevi	OMIM: 600607 MGI: 1202305 HomoloGene: 110905 GeneCards: VPS72
Lokacija gena (čovjek)
Hrom.	Hromosom 1 (čovjek)
Kraj	151,195,321 bp
Lokacija gena (miš)
Hrom.	Hromosom 3 (miš)
Kraj	95,030,362 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• vezivanje sa DNK; • GO:0001948, GO:0016582 vezivanje za proteine; • GO:0031493 histone binding;
Ćelijska komponenta	• jedro; • nukleoplazma; • nuclear speck; • GO:0009327 makromolekulani kompleks;
Biološki proces	• chromatin remodeling; • GO:0009373 regulation of transcription, DNA-templated; • GO:1901227 negative regulation of transcription by RNA polymerase II; • somatic stem cell population maintenance; • transcription, DNA-templated; • GO:0035042 histone exchange; • GO:0031497, GO:0006336, GO:0034724, GO:0001301, GO:0007580, GO:0034652, GO:0010847 chromatin organization;
	Izvori:Amigo / QuickGO
Ortolozi
	6944
	21427
	ENSG00000163159
	ENSMUSG00000008958
	Q15906
	Q62481
	NM_005997; NM_001271087; NM_001271088
	NM_009336
	NP_001258016; NP_001258017; NP_005988
	NP_033362
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Homolog vakuolskog proteina 72 pridruženog sortiranju jest protein koji je kod ljudi kodiran genom VPS72 sa hromosoma 1.^[5]^[6]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 364 aminokiselina, a molekulska težina Da. 40 594^[7]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MSLAGGRAPR	KTAGNRLSGL	LEAEEEDEFY	QTTYGGFTEE	SGDDEYQGDQ
SDTEDEVDSD	FDIDEGDEPS	SDGEAEEPRR	KRRVVTKAYK	EPLKSLRPRK
VNTPAGSSQK	AREEKALLPL	ELQDDGSDSR	KSMRQSTAEH	TRQTFLRVQE
RQGQSRRRKG	PHCERPLTQE	ELLREAKITE	ELNLRSLETY	ERLEADKKKQ
VHKKRKCPGP	IITYHSVTVP	LVGEPGPKEE	NVDIEGLDPA	PSVSALTPHA
GTGPVNPPAR	CSRTFITFSD	DATFEEWFPQ	GRPPKVPVRE	VCPVTHRPAL
YRDPVTDIPY	ATARAFKIIR	EAYKKYITAH	GLPPTASALG	PGPPPPEPLP
GSGPRALRQK	IVIK

Modelni organizmi[uredi | uredi izvor]

U proučavanju funkcije VPS72 korišteni su modelni organizmi. Uslovna nokaut-mišja linija pod nazivom Vps72^{tm1a(EUCOMM)Wtsi} generirana je u Institutu Wellcome Trust Sanger.^[8] > Mužjaci i ženke podvrgnuti su standardizovanom fenotipskom pregledu^[9] za otkrivanja efekta delecija.^[10]^[11]^[12]^[13] Urađeni su dodatni pregledi: Dubinska imunološka fenotipizacija:^[14]

Fenotip *Vps72* nokaut-miševa
Svojstvo	Fenotip
Svi podaci raspoloživi na.^[9]^[14]
Leukociti periferne krvi 6 sedmica	Normalan
Insulin	Normalan
Hematologija 6 sedmica	Normalan
Vijabilnost homozigota na P14	Nenormalan
Studija recesivne letalnosti	Nenormalan
Tjelesna težina	Normalan
Neurološka procjena	Normalan
Snaga stiska	Normalan
Dismorfologija	Normalan
Indirektna kalorimetrija	Normalan
Test tolerancije glukoze	Normalan
Slušni odgovor moždanog stabla	Normalan
DEXA	Normalan
Radiografija	Normalan
Morfologija oka	Normalan
Klinička hemija	Normalan
Hematologija 16 seddmica	Normalan
Leukociti periferne krvi 16 sedmica	Normalan
Težina srca	Normalan
Salmonella infekcija	Normalan
Funkcija citotoksičnih T-ćelija	Normalan
Imunofenotipizacija slezene	Normalan
Imunofenotipizacija mezenternog limfnog čvora	Normalan
Imunofenotipizacija koštane srži	Normal
Kompozicija epidermne imunosti	Normal

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000163159 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000008958 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Horikawa I, Tanaka H, Yuasa Y, Suzuki M, Oshimura M (Mar 1995). "Molecular cloning of a novel human cDNA on chromosome 1q21 and its mouse homolog encoding a nuclear protein with DNA-binding ability". Biochemical and Biophysical Research Communications. 208 (3): 999–1007. doi:10.1006/bbrc.1995.1433. PMID 7702631.
^ "Entrez Gene: VPS72 vacuolar protein sorting 72 homolog (S. cerevisiae)".
^ "UniProt, Q15906" (jezik: en.). Pristupljeno 8. 12. 2021.CS1 održavanje: nepoznati jezik (link)
^ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
^ ^a ^b "International Mouse Phenotyping Consortium".
^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
^ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
^ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
^ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
^ ^a ^b "Infection and Immunity Immunophenotyping (3i) Consortium". Arhivirano s originala, 21. 5. 2015. Pristupljeno 8. 12. 2021.

Doponska literatura[uredi | uredi izvor]

Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Cai Y, Jin J, Florens L, Swanson SK, Kusch T, Li B, Workman JL, Washburn MP, Conaway RC, Conaway JW (Apr 2005). "The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes". The Journal of Biological Chemistry. 280 (14): 13665–70. doi:10.1074/jbc.M500001200. PMID 15647280.
Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Denoeud F, Kapranov P, Ucla C, Frankish A, Castelo R, Drenkow J, Lagarde J, Alioto T, Manzano C, Chrast J, Dike S, Wyss C, Henrichsen CN, Holroyd N, Dickson MC, Taylor R, Hance Z, Foissac S, Myers RM, Rogers J, Hubbard T, Harrow J, Guigó R, Gingeras TR, Antonarakis SE, Reymond A (Jun 2007). "Prominent use of distal 5' transcription start sites and discovery of a large number of additional exons in ENCODE regions". Genome Research. 17 (6): 746–59. doi:10.1101/gr.5660607. PMC 1891335. PMID 17567994.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000163159 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000008958 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid7702631-5] Horikawa I, Tanaka H, Yuasa Y, Suzuki M, Oshimura M (Mar 1995). "Molecular cloning of a novel human cDNA on chromosome 1q21 and its mouse homolog encoding a nuclear protein with DNA-binding ability". Biochemical and Biophysical Research Communications. 208 (3): 999–1007. doi:10.1006/bbrc.1995.1433. PMID 7702631.

[entrez-6] "Entrez Gene: VPS72 vacuolar protein sorting 72 homolog (S. cerevisiae)".

[UniProt-7] "UniProt, Q15906" (jezik: en.). Pristupljeno 8. 12. 2021.CS1 održavanje: nepoznati jezik (link)

[mgp_reference-8] Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.

[IMPCsearch_ref-9] "International Mouse Phenotyping Consortium".

[pmid21677750-10] Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.

[mouse_library-11] Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.

[mouse_for_all_reasons-12] Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.

[pmid23870131-13] White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.

[iii_ref-14] "Infection and Immunity Immunophenotyping (3i) Consortium". Arhivirano s originala, 21. 5. 2015. Pristupljeno 8. 12. 2021.

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