GAPDHS

GAPDHS
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	3H9E, 3PFW, 5C7O, 5C7L
Identifikatori
Aliasi	GAPDHS
Vanjski ID-jevi	OMIM: 609169 MGI: 95653 HomoloGene: 101265 GeneCards: GAPDHS
Lokacija gena (čovjek)
Hrom.	Hromosom 19 (čovjek)
Kraj	35,545,319 bp
Lokacija gena (miš)
Hrom.	Hromosom 7 (miš)
Kraj	30,443,106 bp
Obrazac RNK ekspresije
	; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• oxidoreductase activity; • NAD binding; • oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; • GO:0001948, GO:0016582 vezivanje za proteine; • NADP binding; • glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity;
Ćelijska komponenta	• citoplazma; • citosol; • jedro;
Biološki proces	• flagellated sperm motility; • Glukoneogeneza; • Glikoliza; • positive regulation of glycolytic process; • canonical glycolysis; • glucose metabolic process;
	Izvori:Amigo / QuickGO
Ortolozi
	26330
	14447
	ENSG00000105679
	ENSMUSG00000061099
	O14556
	Q64467
	NM_014364
	NM_001290631; NM_008085
	NP_055179
	NP_001277560; NP_032111
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Spermatogena gliceraldehid-3-fosfat dehidrogenaza, znana i kao testisno specifična gliceraldehide-3-fosfat dehidrogenaza, je enzim koji je kod ljudi kodiran genom GAPDHS.^[5]^[6]

Aminokiselinska sekvenca

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10	20	30	40	50
MSKRDIVLTN	VTVVQLLRQP	CPVTRAPPPP	EPKAEVEPQP	QPEPTPVREE
IKPPPPPLPP	HPATPPPKMV	SVARELTVGI	NGFGRIGRLV	LRACMEKGVK
VVAVNDPFID	PEYMVYMFKY	DSTHGRYKGS	VEFRNGQLVV	DNHEISVYQC
KEPKQIPWRA	VGSPYVVEST	GVYLSIQAAS	DHISAGAQRV	VISAPSPDAP
MFVMGVNEND	YNPGSMNIVS	NASCTTNCLA	PLAKVIHERF	GIVEGLMTTV
HSYTATQKTV	DGPSRKAWRD	GRGAHQNIIP	ASTGAAKAVT	KVIPELKGKL
TGMAFRVPTP	DVSVVDLTCR	LAQPAPYSAI	KEAVKAAAKG	PMAGILAYTE
DEVVSTDFLG	DTHSSIFDAK	AGIALNDNFV	KLISWYDNEY	GYSHRVVDLL
RYMFSRDK

Funkcija[uredi | uredi izvor]

Ovaj gen kodira protein koji pripada gliceraldehid-3-fosfat dehidrogenaza, enzima koji imaju važnu ulogu u metabolizmu ugljikohidrata. Pored ispoljavanja u somatskim ćelijama, ovaj enzim specifičan za spermu, djekuje na nikotinamid adenin-dinukleotid – u zavisnosti od uklanjanja vodika i dodavanja fosfata u gliceraldehid 3-fosfat, da bi se formirao 1,3-difosfoglicerat . Tokom spermiogeneze, ovaj enzim može imati važnu ulogu u regulaciji prebacivanja između različitih puteva za proizvodnju energije, a potreban je za pokretljivost spermatozoida i plodnost muškaraca.^[6]

U melanocitnim ćelijama, ekspresija gena GAPDHS može se regulirati pomoću transkripcijskim faktorom povezanim sa mikroftalmijom (MITF).^[7]

Mapa interaktivnog puta[uredi | uredi izvor]

Šablon:GlycolysisGluconeogenesis WP534

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000105679 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000061099 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Welch JE, Brown PL, O'Brien DA, Magyar PL, Bunch DO, Mori C, Eddy EM (Apr 2000). "Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells". Journal of Andrology. 21 (2): 328–38. doi:10.1002/j.1939-4640.2000.tb02111.x (neaktivno 13. 1. 2021). PMID 10714828.CS1 održavanje: DOI nije aktivan od 2021 (link)
^ ^a ^b "Entrez Gene: GAPDHS glyceraldehyde-3-phosphate dehydrogenase, spermatogenic".
^ Hoek KS, Schlegel NC, Eichhoff OM, Widmer DS, Praetorius C, Einarsson SO, Valgeirsdottir S, Bergsteinsdottir K, Schepsky A, Dummer R, Steingrimsson E (Dec 2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell & Melanoma Research. 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID 19067971. S2CID 24698373.

Dopunska literatura[uredi | uredi izvor]

Cooper TG, Yeung CH (Jun 1999). "Approaches to post-testicular contraception". Asian Journal of Andrology. 1 (1–2): 29–36. PMID 11225901.
Benham FJ, Povey S (Aug 1989). "Members of the human glyceraldehyde-3-phosphate dehydrogenase-related gene family map to dispersed chromosomal locations". Genomics. 5 (2): 209–14. doi:10.1016/0888-7543(89)90048-7. PMID 2793178.
Peterson RN, Freund M (Apr 1973). "Effects of (H + ), (Na + ), (K + ) and certain membrane-active drugs on glycolysis, motility, and ATP synthesis by human spermatozoa". Biology of Reproduction. 8 (3): 350–7. PMID 4349855. Arhivirano s originala (PDF), 23. 9. 2015. Pristupljeno 14. 10. 2011.
Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Westhoff D, Kamp G (Aug 1997). "Glyceraldehyde 3-phosphate dehydrogenase is bound to the fibrous sheath of mammalian spermatozoa". Journal of Cell Science. 110 (15): 1821–9. PMID 9264469.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Goodwin LO, Karabinus DS, Pergolizzi RG, Benoff S (Feb 2000). "L-type voltage-dependent calcium channel alpha-1C subunit mRNA is present in ejaculated human spermatozoa". Molecular Human Reproduction. 6 (2): 127–36. doi:10.1093/molehr/6.2.127. PMID 10655454.
Bone W, Jones AR, Morin C, Nieschlag E, Cooper TG (2001). "Susceptibility of glycolytic enzyme activity and motility of spermatozoa from rat, mouse, and human to inhibition by proven and putative chlorinated antifertility compounds in vitro". Journal of Andrology. 22 (3): 464–70. PMID 11330647.
Suzuki Y, Yamashita R, Shirota M, Sakakibara Y, Chiba J, Mizushima-Sugano J, Nakai K, Sugano S (Sep 2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". Genome Research. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC 515316. PMID 15342556.
Li Y, Nowotny P, Holmans P, Smemo S, Kauwe JS, Hinrichs AL, Tacey K, Doil L, van Luchene R, Garcia V, Rowland C, Schrodi S, Leong D, Gogic G, Chan J, Cravchik A, Ross D, Lau K, Kwok S, Chang SY, Catanese J, Sninsky J, White TJ, Hardy J, Powell J, Lovestone S, Morris JC, Thal L, Owen M, Williams J, Goate A, Grupe A (Nov 2004). "Association of late-onset Alzheimer's disease with genetic variation in multiple members of the GAPD gene family". Proceedings of the National Academy of Sciences of the United States of America. 101 (44): 15688–93. Bibcode:2004PNAS..10115688L. doi:10.1073/pnas.0403535101. PMC 524264. PMID 15507493.
Kodama R, Kondo T, Yokote H, Jing X, Sawada T, Hironishi M, Sakaguchi K (Dec 2005). "Nuclear localization of glyceraldehyde-3-phosphate dehydrogenase is not involved in the initiation of apoptosis induced by 1-Methyl-4-phenyl-pyridium iodide (MPP+)". Genes to Cells. 10 (12): 1211–9. doi:10.1111/j.1365-2443.2005.00911.x. PMID 16324157. S2CID 22847496.
Welch JE, Barbee RR, Magyar PL, Bunch DO, O'Brien DA (Aug 2006). "Expression of the spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase (GAPDS) in rat testis". Molecular Reproduction and Development. 73 (8): 1052–60. doi:10.1002/mrd.20235. PMID 16700075. S2CID 6243286.
Yatsenko AN, Roy A, Chen R, Ma L, Murthy LJ, Yan W, Lamb DJ, Matzuk MM (Dec 2006). "Non-invasive genetic diagnosis of male infertility using spermatozoal RNA: KLHL10 mutations in oligozoospermic patients impair homodimerization". Human Molecular Genetics. 15 (23): 3411–9. doi:10.1093/hmg/ddl417. PMID 17047026.
Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000105679 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000061099 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10714828-5] Welch JE, Brown PL, O'Brien DA, Magyar PL, Bunch DO, Mori C, Eddy EM (Apr 2000). "Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells". Journal of Andrology. 21 (2): 328–38. doi:10.1002/j.1939-4640.2000.tb02111.x (neaktivno 13. 1. 2021). PMID 10714828.CS1 održavanje: DOI nije aktivan od 2021 (link)

[entrez-6] "Entrez Gene: GAPDHS glyceraldehyde-3-phosphate dehydrogenase, spermatogenic".

[pmid19067971-7] Hoek KS, Schlegel NC, Eichhoff OM, Widmer DS, Praetorius C, Einarsson SO, Valgeirsdottir S, Bergsteinsdottir K, Schepsky A, Dummer R, Steingrimsson E (Dec 2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell & Melanoma Research. 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID 19067971. S2CID 24698373.

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