KRTDAP

KRTDAP
Identifikatori
Aliasi	KRTDAP
Vanjski ID-jevi	OMIM: 617212 MGI: 1928282 HomoloGene: 52619 GeneCards: KRTDAP
Lokacija gena (čovjek)
Hrom.	Hromosom 19 (čovjek)
Kraj	35,495,558 bp
Lokacija gena (miš)
Hrom.	Hromosom 7 (miš)
Kraj	30,791,097 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• molekularna funkcija;
Ćelijska komponenta	• extracellular region; • Vanćelijsko; • lamellar granule;
Biološki proces	• Ćelijska diferencijacija; • GO:0022610 biološki proces; • epidermis development;
	Izvori:Amigo / QuickGO
Ortolozi
	388533
	64661
	ENSG00000188508
	ENSMUSG00000074199
	P60985
	Q3V2T4
	NM_207392; NM_001244847
	NM_001033131
	NP_001231776; NP_997275
	NP_001028303; NP_001350938; NP_001350939; NP_001350940
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Pridruženi protein keratinocitne diferencijacije jeprotein koji je kod ljudi kodiran genom KRTDAP .^[5]^[6]

Kloniranje i ekspresija

Iz embrionske epiderme pacova, Oomizu et al. (2000) klonirali su potpuni Krtdap, kojeg su nazvali Kdap, a analizom baze podataka identificirali su tri dodatne varijante prerade. Također su identificirali KDAP miša i čovjeka, za koje se također predviđalo da imaju po četiri varijante prerade. Izvedeni pacovski protein pune dužine od 98 aminokiselina ima izračunatu molekulsku masu od 11 kD, konzerviranu N-terminalnu signalnu sekvencu i dva pretpostavljena mjesta N-miristoilacije. RT-PCR analita je detektirala je četiri prerađene varijante Kdap-a u diferenciranoj embrionskoj epidermi odraslih pacova, sa raslojenim i pseudostratifikiciranim epitelima. [[FISH|Hibridizacija in situ pacovske kože lokalizirala je Kdap u suprabaznom sloju epiderme i suprabaznim ćelijama u infundibulumu dlakinog folikula.

Dio elektroforetske i tkivne ekspresije gena KRTDAP

Koristeći kvantitativnu RT-PCR analizu, Matsui et al. (2004) otkrili su Kdap u slojevitom epitelu iz nekoliko mišjih tkiva, kao i u dušnik]u, mokraćnom mjehuru i timusu. Kdap nije bio otkriven u tipskim jednostavnim epitelima. Hibridizacija in situjastučića mišjeg stopala, otkrila je ekspresiju Kdap ograničenu na suprabazni dio spinoznog sloja. ]][[Epitop-označeni ljudski KDAP je N-terminalno obrađen i izlučen nakon ekspresije u 293 / EBNA-1 ćelijama.

Leclerc et al. (2014) otkrili su da je ekspresija Krtdap-a i suprabasina (SBSN) povećana u koži novorođenih homozigotnih null miševa za varijante dermokina (DMKN) -beta i -gama.^[7]^[8]^[9]

Aminokiselinska sekvenca

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10		20		30		40		50
MKIPVLPAVV		LLSLLVLHSA		QGATLGGPEE		ESTIENYASR		PEAFNTPFLN
IDKLRSAFKA		DEFLNWHALF		ESIKRKLPFL		NWDAFPKLKG		LRSATPDAQ

Reference

1 2 3 GRCh38: Ensembl release 89: ENSG00000188508 - Ensembl, maj 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000074199 - Ensembl, maj 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Oomizu S, Sahuc F, Asahina K, Inamatsu M, Matsuzaki T, Sasaki M, Obara M, Yoshizato K (Dec 2000). "Kdap, a novel gene associated with the stratification of the epithelium". Gene. 256 (1–2): 19–27. doi:10.1016/S0378-1119(00)00357-7. PMID 11054531.
↑ "Entrez Gene: UNQ467 KIPV467".
↑ Leclerc, E. A., Huchenq, A., Kezic, S., Serre, G., Jonca, N. Mice deficient for the epidermal dermokine beta and gamma isoforms display transient cornification defects. J. Cell Sci. 127: 2862-2872, 2014. PubMed: 24794495
↑ Matsui, T., Hayashi-Kisumi, F., Kinoshita, Y., Katahira, S., Morita, K., Miyachi, Y., Ono, Y., Imai, T., Tanigawa, Y., Komiya, T., Tsukita, S. Identification of novel keratinocyte-secreted peptides dermokine-alpha/-beta and a new stratified epithelium-secreted protein gene complex on human chromosome 19q13.1. Genomics 84: 384-397, 2004. [PubMed]: 15234001
↑ Oomizu, S., Sahuc, F., Asahina, K., Inamatsu, M., Matsuzaki, T., Sasaki, M., Obara, M., Yoshizato, K. Kdap, a novel gene associated with the stratification of the epithelium. Gene 256: 19-27, 2000. [PubMed]: 11054531

Dopunska literatura

Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
Tsuchida S, Bonkobara M, McMillan JR, et al. (2004). "Characterization of Kdap, a protein secreted by keratinocytes". J. Invest. Dermatol. 122 (5): 1225–34. doi:10.1111/j.0022-202X.2004.22511.x. PMID 15140226.
Clark HF, Gurney AL, Abaya E, et al. (2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and Transmembrane Proteins: A Bioinformatics Assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Adams MD, Kerlavage AR, Fleischmann RD, et al. (1995). "Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence" (PDF). Nature. 377 (6547 Suppl): 3–174. PMID 7566098.

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000188508 - Ensembl, maj 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000074199 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid11054531-5] Oomizu S, Sahuc F, Asahina K, Inamatsu M, Matsuzaki T, Sasaki M, Obara M, Yoshizato K (Dec 2000). "Kdap, a novel gene associated with the stratification of the epithelium". Gene. 256 (1–2): 19–27. doi:10.1016/S0378-1119(00)00357-7. PMID 11054531.

[entrez-6] "Entrez Gene: UNQ467 KIPV467".

[7] Leclerc, E. A., Huchenq, A., Kezic, S., Serre, G., Jonca, N. Mice deficient for the epidermal dermokine beta and gamma isoforms display transient cornification defects. J. Cell Sci. 127: 2862-2872, 2014. PubMed: 24794495

[8] Matsui, T., Hayashi-Kisumi, F., Kinoshita, Y., Katahira, S., Morita, K., Miyachi, Y., Ono, Y., Imai, T., Tanigawa, Y., Komiya, T., Tsukita, S. Identification of novel keratinocyte-secreted peptides dermokine-alpha/-beta and a new stratified epithelium-secreted protein gene complex on human chromosome 19q13.1. Genomics 84: 384-397, 2004. [PubMed]: 15234001

[9] Oomizu, S., Sahuc, F., Asahina, K., Inamatsu, M., Matsuzaki, T., Sasaki, M., Obara, M., Yoshizato, K. Kdap, a novel gene associated with the stratification of the epithelium. Gene 256: 19-27, 2000. [PubMed]: 11054531

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