LCMT2
Leucin-karboksil metiltransferaza 2 je enzim koji je kod ljudi kodiran genom LCMT2.[5][6][7]
Protein koji je kodiran ovim genom bez introna pripada superporodici metiltransferaza i djeluje kao regulator kontrolne tačke G(1)/S i G(2)/M. Pretpostavlja se da oksidativni stres izazvan dimom cigareta i transformirajući faktor rasta beta 1 mogu inhibirati ćelijsku proliferaciju, modulirajući ekspresiju ovog proteina.[7]
Hibridnom analizom zračenja, Nagase et al. (1998) mapirali su gen LCMT2 na hromosom 4. Međutim, Hartz (2013) ga je mapirala je na hromosom 15, sekvenca q15.3, a na osnovu poravnanja sekvence LCMT2 (GenBank AF265443) sa genomskom sekvencom (GRCh37).[8][9]
Aminokiselinska sekvenca
[uredi | uredi izvor]Dužina polipeptidnog lanca je 686 aminokiselina, a molekulska težina 75.602 Da .[10].
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MGPRSRERRA | GAVQNTNDSS | ALSKRSLAAR | GYVQDPFAAL | LVPGAARRAP | ||||
| LIHRGYYVRA | RAVRHCVRAF | LEQIGAPQAA | LRAQILSLGA | GFDSLYFRLK | ||||
| TAGRLARAAV | WEVDFPDVAR | RKAERIGETP | ELCALTGPFE | RGEPASALCF | ||||
| ESADYCILGL | DLRQLQRVEE | ALGAAGLDAA | SPTLLLAEAV | LTYLEPESAA | ||||
| ALIAWAAQRF | PNALFVVYEQ | MRPQDAFGQF | MLQHFRQLNS | PLHGLERFPD | ||||
| VEAQRRRFLQ | AGWTACGAVD | MNEFYHCFLP | AEERRRVENI | EPFDEFEEWH | ||||
| LKCAHYFILA | ASRGDTLSHT | LVFPSSEAFP | RVNPASPSGV | FPASVVSSEG | ||||
| QVPNLKRYGH | ASVFLSPDVI | LSAGGFGEQE | GRHCRVSQFH | LLSRDCDSEW | ||||
| KGSQIGSCGT | GVQWDGRLYH | TMTRLSESRV | LVLGGRLSPV | SPALGVLQLH | ||||
| FFKSEDNNTE | DLKVTITKAG | RKDDSTLCCW | RHSTTEVSCQ | NQEYLFVYGG | ||||
| RSVVEPVLSD | WHFLHVGTMA | WVRIPVEGEV | PEARHSHSAC | TWQGGALIAG | ||||
| GLGASEEPLN | SVLFLRPISC | GFLWESVDIQ | PPITPRYSHT | AHVLNGKLLL | ||||
| VGGIWIHSSS | FPGVTVINLT | TGLSSEYQID | TTYVPWPLML | HNHTSILLPE | ||||
| EQQLLLLGGG | GNCFSFGTYF | NPHTVTLDLS | SLSAGQ |
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
Kloniranje i ekspresija
[uredi | uredi izvor]Sekvenciranjem klonova dobijenih iz biblioteke moždane cDNK Nagase et al. (1998) klonirali su LCMT2, koji su označili KIAA0547. Transkript sadrži nekoliko ponavljajućih elemenata u svom 3-baznom UTR-u, a izvedeni protein sadrži 686 aminokiselina. RT-PCR je detektirao ekspresiju LCMT2 samo u bubrezima. In vitro translatirani LCMT3 imao je prividnu molekulsku masu od 61 kD, pomoću SDS-PAGE.
Reference
[uredi | uredi izvor]- ^ a b c GRCh38: Ensembl release 89: ENSG00000168806 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000074890 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Aug 1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (1): 31–9. doi:10.1093/dnares/5.1.31. PMID 9628581.
- ^ Noma A, Suzuki T (Dec 2006). "Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis". Nucleic Acids Symp Ser (Oxf). 50 (50): 65–6. doi:10.1093/nass/nrl032. PMID 17150819.
- ^ a b "Entrez Gene: LCMT2 leucine carboxyl methyltransferase 2".
- ^ Hartz, P. A. Personal Communication. Baltimore, Md. 6/3/2013.
- ^ Nagase, T., Ishikawa, K., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 31-39, 1998. PubMed: 9628581
- ^ "UniProt, O60294". Pristupljeno 15. 7. 2021.
Dopunska literatura
[uredi | uredi izvor]- De Baere I, Derua R, Janssens V, et al. (2000). "Purification of porcine brain protein phosphatase 2A leucine carboxyl methyltransferase and cloning of the human homologue". Biochemistry. 38 (50): 16539–47. doi:10.1021/bi991646a. PMID 10600115.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Marwick JA, Kirkham P, Gilmour PS, et al. (2003). "Cigarette smoke-induced oxidative stress and TGF-beta1 increase p21waf1/cip1 expression in alveolar epithelial cells". Ann. N. Y. Acad. Sci. 973: 278–83. doi:10.1111/j.1749-6632.2002.tb04649.x. PMID 12485877.
- Ng CC, Arakawa H, Fukuda S, et al. (2003). "p53RFP, a p53-inducible RING-finger protein, regulates the stability of p21WAF1". Oncogene. 22 (28): 4449–58. doi:10.1038/sj.onc.1206586. PMID 12853982.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Kawamata N, Inagaki N, Mizumura S, et al. (2005). "Methylation status analysis of cell cycle regulatory genes (p16INK4A, p15INK4B, p21Waf1/Cip1, p27Kip1 and p73) in natural killer cell disorders". Eur. J. Haematol. 74 (5): 424–9. doi:10.1111/j.1600-0609.2005.00417.x. PMID 15813917.
- Noma A, Kirino Y, Ikeuchi Y, Suzuki T (2006). "Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA". EMBO J. 25 (10): 2142–54. doi:10.1038/sj.emboj.7601105. PMC 1462984. PMID 16642040.
- Liu FY, Qi JP, Xu FL, Wu AP (2006). "Clinicopathological and immunohistochemical analysis of gastrointestinal stromal tumor". World J. Gastroenterol. 12 (26): 4161–5. PMID 16830365.
- Moon SK, Choi YH, Kim CH, Choi WS (2006). "p38MAPK mediates benzyl isothiocyanate-induced p21WAF1 expression in vascular smooth muscle cells via the regulation of Sp1". Biochem. Biophys. Res. Commun. 350 (3): 662–8. doi:10.1016/j.bbrc.2006.09.092. PMID 17026958.