CHP (gen)

CHP (gen)
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2E30
Identifikatori
Aliasi	CHP1
Vanjski ID-jevi	OMIM: 606988 MGI: 1927185 HomoloGene: 5235 GeneCards: CHP1
Lokacija gena (čovjek)
Hrom.	Hromosom 15 (čovjek)
Kraj	41,281,887 bp
Lokacija gena (miš)
Hrom.	Hromosom 2 (miš)
Kraj	119,417,508 bp
Obrazac RNK ekspresije
	; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• calcium ion binding; • transporter activity; • microtubule binding; • protein kinase inhibitor activity; • vezivanje iona metala; • calcium-dependent protein binding; • potassium channel regulator activity; • kinase binding; • GO:0001948, GO:0016582 vezivanje za proteine;
Ćelijska komponenta	• citoplazma; • citosol; • membrana; • focal adhesion; • microtubule cytoskeleton; • Golđijeva membrana; • ćelijska membrana; • transport vesicle; • Endoplazmatski retikulum; • Egzosom; • citoskelet; • Endomembranski sistem; • jedro; • endoplasmic reticulum-Golgi intermediate compartment;
Biološki proces	• GO:0033128 negative regulation of protein phosphorylation; • GO:0090284 positive regulation of protein glycosylation; • negative regulation of calcineurin-NFAT signaling cascade; • hyaluronan catabolic process; • negative regulation of protein autophosphorylation; • negative regulation of protein kinase activity; • positive regulation of sodium:proton antiporter activity; • negative regulation of phosphatase activity; • small GTPase mediated signal transduction; • protein stabilization; • cellular response to acidic pH; • membrane fusion; • regulation of intracellular pH; • positive regulation of protein transport; • negative regulation of protein ubiquitination; • potassium ion transport; • protein complex oligomerization; • microtubule bundle formation; • cytoplasmic microtubule organization; • protein export from nucleus; • membrane docking; • regulation of neuron death; • protein transport; • membrane organization; • negative regulation of NF-kappaB transcription factor activity; • calcium-ion regulated exocytosis; • positive regulation of protein targeting to membrane; • negative regulation of protein import into nucleus; • GO:0015915 transport;
	Izvori:Amigo / QuickGO
Ortolozi
	11261
	56398
	ENSG00000187446
	ENSMUSG00000014077
	Q99653
	P61022
	NM_007236
	NM_019769
	NP_009167
	NP_062743
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Kalcineurin B homologni protein 1 je protein koji je kod ljudi kodiran genom CHP1 (ranija CHP).^[5]

Gen CHP1 kodira bitni kofaktor SLC9A1 (NHE1), izmjenjivača Na+/H+ koji regulira unutarćelijske ione i homeostazu pH. Međunarodni radijacijski konzorcij za hibridno mapiranje locirao je gen CHP na hromosom 15 (stSG38846).

Kloniranje i ekspresija[uredi | uredi izvor]

Više signalnih puteva regulira Na+/H+ proteine koji se transportuju. Da bi identificirali regulatorne proteine, Lin i Barber (1996) koristili su C-terminalni citoplazmatski domen sveprisutno eksprimirane izoforme NHE1 za pregled biblioteke ekspresije ljudskih B-ćelija. Djelimična sekvenca dobijena s ovog snimka korištena je za kloniranje nove cDNK pune dužine, označene kao CHP, iz druge B-ćelijske biblioteke. Izvedeni protein od 195 aminokiselina sadrži dva različita i dva potencijalna motiva vezana za [kalcij|[Ca⁽²⁺⁾ za EF-šaku i dijeli 43% i 33% identiteta s kalcineurinom B i kalmodulinom. Također ima 99% identiteta sekvence sa homologom pacova. Northern blot analiza pokazala je ekspresiju 4-kb CHP transkripta u svih osam ispitivanih fetush tkiva.

U mišjem malom mozgu, pronašli su ekspresiju gena Chp1 na presinapsnim terminalima Purkinjeovih ćelija, kao i na aksonskim terminalima drugih neurona.

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 195 aminokiselina, a molekulska težina 22.456 Da.^[6].

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10	20	30	40	50
MGSRASTLLR	DEELEEIKKE	TGFSHSQITR	LYSRFTSLDK	GENGTLSRED
FQRIPELAIN	PLGDRIINAF	FPEGEDQVNF	RGFMRTLAHF	RPIEDNEKSK
DVNGPEPLNS	RSNKLHFAFR	LYDLDKDEKI	SRDELLQVLR	MMVGVNISDE
QLGSIADRTI	QEADQDGDSA	ISFTEFVKVL	EKVDVEQKMS	IRFLH

Funkcija[uredi | uredi izvor]

Protein koji kodira ovaj gen je fosfoprotein koji se veže za izmjenjivače natrij-vodik (NHE). Ovaj protein služi kao esencijalni kofaktor koji podržava fiziološku aktivnost članova porodice NHE. Ima sličnost proteinske sekvence s kalcineurinom B, a poznato je i da je endogeni inhibitor aktivnosti kalcineurina.^[5]

Interakcije[uredi | uredi izvor]

Pokazalo se da homologni protein 1 kalcineurina B ima interakcija sa

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000187446 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000014077 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ^a ^b "Entrez Gene: CHP calcium binding protein P22".
^ "UniProt, Q99653". Pristupljeno 12. 7. 2021.
^ ^a ^b Inoue H, Nakamura Y, Nagita M, Takai T, Masuda M, Nakamura N, Kanazawa H (Feb 2003). "Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium". Biological & Pharmaceutical Bulletin. 26 (2): 148–55. doi:10.1248/bpb.26.148. PMID 12576672.
^ Lin X, Barber DL (Oct 1996). "A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange". Proceedings of the National Academy of Sciences of the United States of America. 93 (22): 12631–6. doi:10.1073/pnas.93.22.12631. PMC 38044. PMID 8901634.
^ Pang T, Su X, Wakabayashi S, Shigekawa M (maj 2001). "Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers". The Journal of Biological Chemistry. 276 (20): 17367–72. doi:10.1074/jbc.M100296200. PMID 11350981.
^ Zhang-James Y, DasBanerjee T, Sagvolden T, Middleton FA, Faraone SV (Dec 2011). "SLC9A9 mutations, gene expression, and protein-protein interactions in rat models of attention-deficit/hyperactivity disorder". American Journal of Medical Genetics Part B. 156B (7): 835–43. doi:10.1002/ajmg.b.31229. PMC 3168688. PMID 21858920.

Dopunsdka literatura[uredi | uredi izvor]

Robertson NG, Khetarpal U, Gutiérrez-Espeleta GA, Bieber FR, Morton CC (Sep 1994). "Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening". Genomics. 23 (1): 42–50. doi:10.1006/geno.1994.1457. PMID 7829101.
Barroso MR, Bernd KK, DeWitt ND, Chang A, Mills K, Sztul ES (Apr 1996). "A novel Ca2+-binding protein, p22, is required for constitutive membrane traffic". The Journal of Biological Chemistry. 271 (17): 10183–7. doi:10.1074/jbc.271.17.10183. PMID 8626580.
Lin X, Barber DL (Oct 1996). "A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange". Proceedings of the National Academy of Sciences of the United States of America. 93 (22): 12631–6. doi:10.1073/pnas.93.22.12631. PMC 38044. PMID 8901634.
Lin X, Sikkink RA, Rusnak F, Barber DL (Dec 1999). "Inhibition of calcineurin phosphatase activity by a calcineurin B homologous protein". The Journal of Biological Chemistry. 274 (51): 36125–31. doi:10.1074/jbc.274.51.36125. PMID 10593895.
Pang T, Su X, Wakabayashi S, Shigekawa M (maj 2001). "Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers". The Journal of Biological Chemistry. 276 (20): 17367–72. doi:10.1074/jbc.M100296200. PMID 11350981.
Matsumoto M, Miyake Y, Nagita M, Inoue H, Shitakubo D, Takemoto K, Ohtsuka C, Murakami H, Nakamura N, Kanazawa H (Aug 2001). "A serine/threonine kinase which causes apoptosis-like cell death interacts with a calcineurin B-like protein capable of binding Na(+)/H(+) exchanger". Journal of Biochemistry. 130 (2): 217–25. doi:10.1093/oxfordjournals.jbchem.a002975. PMID 11481038.
Nakamura N, Miyake Y, Matsushita M, Tanaka S, Inoue H, Kanazawa H (Sep 2002). "KIF1Bbeta2, capable of interacting with CHP, is localized to synaptic vesicles". Journal of Biochemistry. 132 (3): 483–91. doi:10.1093/oxfordjournals.jbchem.a003246. PMID 12204119.
Pang T, Wakabayashi S, Shigekawa M (Nov 2002). "Expression of calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger". The Journal of Biological Chemistry. 277 (46): 43771–7. doi:10.1074/jbc.M208313200. PMID 12226101.
Inoue H, Nakamura Y, Nagita M, Takai T, Masuda M, Nakamura N, Kanazawa H (Feb 2003). "Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium". Biological & Pharmaceutical Bulletin. 26 (2): 148–55. doi:10.1248/bpb.26.148. PMID 12576672.
Pang T, Hisamitsu T, Mori H, Shigekawa M, Wakabayashi S (Mar 2004). "Role of calcineurin B homologous protein in pH regulation by the Na+/H+ exchanger 1: tightly bound Ca2+ ions as important structural elements". Biochemistry. 43 (12): 3628–36. doi:10.1021/bi0360004. PMID 15035633.
Andrade J, Pearce ST, Zhao H, Barroso M (Dec 2004). "Interactions among p22, glyceraldehyde-3-phosphate dehydrogenase and microtubules". The Biochemical Journal. 384 (Pt 2): 327–36. doi:10.1042/BJ20040622. PMC 1134116. PMID 15312048.
Ben Ammar Y, Takeda S, Sugawara M, Miyano M, Mori H, Wakabayashi S (Oct 2005). "Crystallization and preliminary crystallographic analysis of the human calcineurin homologous protein CHP2 bound to the cytoplasmic region of the Na+/H+ exchanger NHE1". Acta Crystallographica Section F. 61 (Pt 10): 956–8. doi:10.1107/S1744309105030836. PMC 1991313. PMID 16511206.
Mishima M, Wakabayashi S, Kojima C (Jan 2007). "Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1". The Journal of Biological Chemistry. 282 (4): 2741–51. doi:10.1074/jbc.M604092200. PMID 17050540.

Vanjski linkovi[uredi | uredi izvor]

Lokacija ljudskog genoma CHP1 i stranica sa detaljima o genu CHP1 u UCSC Genome Browseru.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000187446 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000014077 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] "Entrez Gene: CHP calcium binding protein P22".

[UniProt-6] "UniProt, Q99653". Pristupljeno 12. 7. 2021.

[pmid12576672-7] Inoue H, Nakamura Y, Nagita M, Takai T, Masuda M, Nakamura N, Kanazawa H (Feb 2003). "Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium". Biological & Pharmaceutical Bulletin. 26 (2): 148–55. doi:10.1248/bpb.26.148. PMID 12576672.

[pmid8901634-8] Lin X, Barber DL (Oct 1996). "A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange". Proceedings of the National Academy of Sciences of the United States of America. 93 (22): 12631–6. doi:10.1073/pnas.93.22.12631. PMC 38044. PMID 8901634.

[pmid11350981-9] Pang T, Su X, Wakabayashi S, Shigekawa M (maj 2001). "Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers". The Journal of Biological Chemistry. 276 (20): 17367–72. doi:10.1074/jbc.M100296200. PMID 11350981.

[pmid21858920-10] Zhang-James Y, DasBanerjee T, Sagvolden T, Middleton FA, Faraone SV (Dec 2011). "SLC9A9 mutations, gene expression, and protein-protein interactions in rat models of attention-deficit/hyperactivity disorder". American Journal of Medical Genetics Part B. 156B (7): 835–43. doi:10.1002/ajmg.b.31229. PMC 3168688. PMID 21858920.

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