NUDT4
Difosfoinozitol polifosfat-fosfohidrolaza 2 je enzim koji je kod ljudi kodiran genom NUDT4.[5][6][7]
Protein kodiran ovim genom regulira promet polifosfat-difosfoinozitola. Promet ovih visokoenergetskih difosfoinozitol-polifosfata predstavlja aktivnost promjene molekula sa važnim regulatornim posljedicama. Molekulskno prebacivanje difosfoinozitol-polifosfata može doprinijeti regulaciji unutarčelijskog prometa. Opisano je nekoliko alternativno prerađenih varijanti transkripta, ali priroda nekih od njih nije utvrđena cijelom dužinom. Izoforme DIPP2alfa i DIPP2beta međusobno se razlikuju samo po tome što DIPP2beta ima jednu dodatnu aminokiselinu zbog pomjeranja granice introna u alternativnoj preradi.[7]
Aminokiselinska sekvenca
[uredi | uredi izvor]Dužina polipeptidnog lanca je 180 aminokiselina, a molekulska težina 20.306 Da.[8].
10 | 20 | 30 | 40 | 50 | ||||
---|---|---|---|---|---|---|---|---|
MMKFKPNQTR | TYDREGFKKR | AACLCFRSEQ | EDEVLLVSSS | RYPDQWIVPG | ||||
GGMEPEEEPG | GAAVREVYEE | AGVKGKLGRL | LGIFENQDRK | HRTYVYVLTV | ||||
TEILEDWEDS | VNIGRKREWF | KVEDAIKVLQ | CHKPVHAEYL | EKLKLGCSPA | ||||
NGNSTVPSLP | DNNALFVTAA | QTSGLPSSVR |
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
Reference
[uredi | uredi izvor]- ^ a b c GRCh38: Ensembl release 89: ENSG00000173598 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000020029 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Caffrey JJ, Safrany ST, Yang X, Shears SB (Jun 2000). "Discovery of molecular and catalytic diversity among human diphosphoinositol-polyphosphate phosphohydrolases. An expanding Nudt family". J Biol Chem. 275 (17): 12730–6. doi:10.1074/jbc.275.17.12730. PMID 10777568.
- ^ Caffrey JJ, Shears SB (maj 2001). "Genetic rationale for microheterogeneity of human diphosphoinositol polyphosphate phosphohydrolase type 2". Gene. 269 (1–2): 53–60. doi:10.1016/S0378-1119(01)00446-2. PMID 11376937.
- ^ a b "Entrez Gene: NUDT4 nudix (nucleoside diphosphate linked moiety X)-type motif 4".
- ^ "UniProt, Q9NZJ9". Pristupljeno 12. 8. 2021.
Dopunska literatura
[uredi | uredi izvor]- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Seki N, Ohira M, Nagase T, et al. (1998). "Characterization of cDNA clones in size-fractionated cDNA libraries from human brain". DNA Res. 4 (5): 345–9. doi:10.1093/dnares/4.5.345. PMID 9455484.
- Leslie NR, McLennan AG, Safrany ST (2002). "Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases". BMC Biochem. 3: 20. doi:10.1186/1471-2091-3-20. PMC 117780. PMID 12121577.
- Fisher DI, Safrany ST, Strike P, et al. (2003). "Nudix hydrolases that degrade dinucleoside and diphosphoinositol polyphosphates also have 5-phosphoribosyl 1-pyrophosphate (PRPP) pyrophosphatase activity that generates the glycolytic activator ribose 1,5-bisphosphate". J. Biol. Chem. 277 (49): 47313–7. doi:10.1074/jbc.M209795200. PMID 12370170.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Fortna A, Kim Y, MacLaren E, et al. (2006). "Lineage-Specific Gene Duplication and Loss in Human and Great Ape Evolution". PLOS Biol. 2 (7): E207. doi:10.1371/journal.pbio.0020207. PMC 449870. PMID 15252450.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.