CRTC2

CRTC2
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	4HTM
Identifikatori
Aliasi	CRTC2
Vanjski ID-jevi	OMIM: 608972 MGI: 1921593 HomoloGene: 18765 GeneCards: CRTC2
Lokacija gena (čovjek)
Hrom.	Hromosom 1 (čovjek)
Kraj	153,958,615 bp
Lokacija gena (miš)
Hrom.	Hromosom 3 (miš)
Kraj	90,171,432 bp
Ontologija gena
Molekularna funkcija	• chromatin binding; • GO:0001948, GO:0016582 vezivanje za proteine; • cAMP response element binding protein binding;
Ćelijska komponenta	• citoplazma; • Egzosom; • jedro; • nukleoplazma;
Biološki proces	• Glukoneogeneza; • histone H3-K9 acetylation; • positive regulation of CREB transcription factor activity; • GO:0022415 viral process; • GO:0009373 regulation of transcription, DNA-templated; • protein homotetramerization; • toxin transport; • glucose homeostasis; • transcription, DNA-templated; • GO:0003257, GO:0010735, GO:1901228, GO:1900622, GO:1904488 positive regulation of transcription by RNA polymerase II;
	Izvori:Amigo / QuickGO
Ortolozi
	200186
	74343
	ENSG00000160741
	ENSMUSG00000027936
	Q53ET0
	Q3U182
	NM_181715
	NM_028881; NM_001357152
	NP_859066
	NP_083157; NP_001344081
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

CREB-regulirani transkripcijski koaktivator 2, znan i kao CRTC2, jest protein koji je kod ljudi kodiran genom CRTC2 sa hromosoma 1.^[5]^[6]^[7]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 693 aminokiseline, a molekulska težina 73.302 Da.^[8]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MATSGANGPG	SATASASNPR	KFSEKIALQK	QRQAEETAAF	EEVMMDIGST
RLQAQKLRLA	YTRSSHYGGS	LPNVNQIGSG	LAEFQSPLHS	PLDSSRSTRH
HGLVERVQRD	PRRMVSPLRR	YTRHIDSSPY	SPAYLSPPPE	SSWRRTMAWG
NFPAEKGQLF	RLPSALNRTS	SDSALHTSVM	NPSPQDTYPG	PTPPSILPSR
RGGILDGEMD	PKVPAIEENL	LDDKHLLKPW	DAKKLSSSSS	RPRSCEVPGI
NIFPSPDQPA	NVPVLPPAMN	TGGSLPDLTN	LHFPPPLPTP	LDPEETAYPS
LSGGNSTSNL	THTMTHLGIS	RGMGLGPGYD	APGLHSPLSH	PSLQSSLSNP
NLQASLSSPQ	PQLQGSHSHP	SLPASSLARH	VLPTTSLGHP	SLSAPALSSS
SSSSSTSSPV	LGAPSYPAST	PGASPHHRRV	PLSPLSLLAG	PADARRSQQQ
LPKQFSPTMS	PTLSSITQGV	PLDTSKLSTD	QRLPPYPYSS	PSLVLPTQPH
TPKSLQQPGL	PSQSCSVQSS	GGQPPGRQSH	YGTPYPPGPS	GHGQQSYHRP
MSDFNLGNLE	QFSMESPSAS	LVLDPPGFSE	GPGFLGGEGP	MGGPQDPHTF
NHQNLTHCSR	HGSGPNIILT	GDSSPGFSKE	IAAALAGVPG	FEVSAAGLEL
GLGLEDELRM	EPLGLEGLNM	LSDPCALLPD	PAVEESFRSD	RLQ

Funkcija[uredi | uredi izvor]

CRTC2, prvobitno zvani TORC2, je transkripcijski koaktivator za faktor transkripcije CREB i centralni regulator glukoneogene ekspresije gena, kao odgovora na cAMP.^[9] Smatra se da CRTC2 pokreće tumorigenezu kod LKB11/STK11-null karcinoma nemalih ćelija pluća (NSCLC).^[10]

Interakcije[uredi | uredi izvor]

Pokazalo se da CRTC2 ima interakcije sa SNF1LK2^[11] i YWHAQ.^[11]^[12]

Reference[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000160741 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000027936 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Entrez Gene: CRTC2 CREB regulated transcription coactivator 2".
^ Iourgenko V, Zhang W, Mickanin C, Daly I, Jiang C, Hexham JM, et al. (oktobar 2003). "Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells". Proceedings of the National Academy of Sciences of the United States of America. 100 (21): 12147–52. doi:10.1073/pnas.1932773100. PMC 218727. PMID 14506290.
^ Conkright MD, Canettieri G, Screaton R, Guzman E, Miraglia L, Hogenesch JB, Montminy M (august 2003). "TORCs: transducers of regulated CREB activity". Molecular Cell. 12 (2): 413–23. doi:10.1016/j.molcel.2003.08.013. PMID 14536081.
^ "UniProt, Q53ET0" (jezik: en.). Pristupljeno 9. 12. 2021.CS1 održavanje: nepoznati jezik (link)
^ Cheng A, Saltiel AR (mart 2006). "More TORC for the gluconeogenic engine". BioEssays. 28 (3): 231–4. CiteSeerX 10.1.1.604.5745. doi:10.1002/bies.20375. PMID 16479585.
^ Rodón L, Svensson RU, Wiater E, Chun MG, Tsai WW, Eichner LJ, et al. (juli 2019). "The CREB coactivator CRTC2 promotes oncogenesis in LKB1-mutant non-small cell lung cancer". Science Advances. 5 (7): eaaw6455. doi:10.1126/sciadv.aaw6455. PMC 6656544. PMID 31355336.
^ ^a ^b Screaton RA, Conkright MD, Katoh Y, Best JL, Canettieri G, Jeffries S, et al. (oktobar 2004). "The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector". Cell. 119 (1): 61–74. doi:10.1016/j.cell.2004.09.015. PMID 15454081. S2CID 18616459.
^ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

Dopunska literatura[uredi | uredi izvor]

Maruyama K, Sugano S (januar 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (oktobar 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Iourgenko V, Zhang W, Mickanin C, Daly I, Jiang C, Hexham JM, et al. (oktobar 2003). "Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells". Proceedings of the National Academy of Sciences of the United States of America. 100 (21): 12147–52. doi:10.1073/pnas.1932773100. PMC 218727. PMID 14506290.
Conkright MD, Canettieri G, Screaton R, Guzman E, Miraglia L, Hogenesch JB, Montminy M (august 2003). "TORCs: transducers of regulated CREB activity". Molecular Cell. 12 (2): 413–23. doi:10.1016/j.molcel.2003.08.013. PMID 14536081.
Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, et al. (august 2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Current Biology. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660. S2CID 2371325.
Screaton RA, Conkright MD, Katoh Y, Best JL, Canettieri G, Jeffries S, et al. (oktobar 2004). "The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector". Cell. 119 (1): 61–74. doi:10.1016/j.cell.2004.09.015. PMID 15454081. S2CID 18616459.
Al-Hakim AK, Göransson O, Deak M, Toth R, Campbell DG, Morrice NA, et al. (decembar 2005). "14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK". Journal of Cell Science. 118 (Pt 23): 5661–73. doi:10.1242/jcs.02670. PMID 16306228.
Siu YT, Chin KT, Siu KL, Yee Wai Choy E, Jeang KT, Jin DY (juli 2006). "TORC1 and TORC2 coactivators are required for tax activation of the human T-cell leukemia virus type 1 long terminal repeats". Journal of Virology. 80 (14): 7052–9. doi:10.1128/JVI.00103-06. PMC 1489057. PMID 16809310.
Wu Z, Huang X, Feng Y, Handschin C, Feng Y, Gullicksen PS, et al. (septembar 2006). "Transducer of regulated CREB-binding proteins (TORCs) induce PGC-1alpha transcription and mitochondrial biogenesis in muscle cells". Proceedings of the National Academy of Sciences of the United States of America. 103 (39): 14379–84. Bibcode:2006PNAS..10314379W. doi:10.1073/pnas.0606714103. PMC 1569674. PMID 16980408.
Yang Q, Inoki K, Ikenoue T, Guan KL (oktobar 2006). "Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity". Genes & Development. 20 (20): 2820–32. doi:10.1101/gad.1461206. PMC 1619946. PMID 17043309.
Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
Ravnskjaer K, Kester H, Liu Y, Zhang X, Lee D, Yates JR, Montminy M (juni 2007). "Cooperative interactions between CBP and TORC2 confer selectivity to CREB target gene expression". The EMBO Journal. 26 (12): 2880–9. doi:10.1038/sj.emboj.7601715. PMC 1894761. PMID 17476304.
Kuraishy AI, French SW, Sherman M, Herling M, Jones D, Wall R, Teitell MA (juni 2007). "TORC2 regulates germinal center repression of the TCL1 oncoprotein to promote B cell development and inhibit transformation". Proceedings of the National Academy of Sciences of the United States of America. 104 (24): 10175–80. Bibcode:2007PNAS..10410175K. doi:10.1073/pnas.0704170104. PMC 1891214. PMID 17548807.

Vanjski linkovi[uredi | uredi izvor]

CRTC2 protein, human na US National Library of Medicine Medical Subject Headings (MeSH)
Lokacija ljudskog genoma CRTC2 i stranica sa detaljima o genu CRTC2 u UCSC Genome Browseru.
Q53ET0
Q3U182

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000160741 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000027936 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] "Entrez Gene: CRTC2 CREB regulated transcription coactivator 2".

[pmid14506290-6] Iourgenko V, Zhang W, Mickanin C, Daly I, Jiang C, Hexham JM, et al. (oktobar 2003). "Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells". Proceedings of the National Academy of Sciences of the United States of America. 100 (21): 12147–52. doi:10.1073/pnas.1932773100. PMC 218727. PMID 14506290.

[pmid14536081-7] Conkright MD, Canettieri G, Screaton R, Guzman E, Miraglia L, Hogenesch JB, Montminy M (august 2003). "TORCs: transducers of regulated CREB activity". Molecular Cell. 12 (2): 413–23. doi:10.1016/j.molcel.2003.08.013. PMID 14536081.

[UniProt-8] "UniProt, Q53ET0" (jezik: en.). Pristupljeno 9. 12. 2021.CS1 održavanje: nepoznati jezik (link)

[pmid16479585-9] Cheng A, Saltiel AR (mart 2006). "More TORC for the gluconeogenic engine". BioEssays. 28 (3): 231–4. CiteSeerX 10.1.1.604.5745. doi:10.1002/bies.20375. PMID 16479585.

[10] Rodón L, Svensson RU, Wiater E, Chun MG, Tsai WW, Eichner LJ, et al. (juli 2019). "The CREB coactivator CRTC2 promotes oncogenesis in LKB1-mutant non-small cell lung cancer". Science Advances. 5 (7): eaaw6455. doi:10.1126/sciadv.aaw6455. PMC 6656544. PMID 31355336.

[pmid15454081-11] Screaton RA, Conkright MD, Katoh Y, Best JL, Canettieri G, Jeffries S, et al. (oktobar 2004). "The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector". Cell. 119 (1): 61–74. doi:10.1016/j.cell.2004.09.015. PMID 15454081. S2CID 18616459.

[pmid17353931-12] Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

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