Kalpain-1

Kalpain-1
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	1ZCM, 2ARY
Identifikatori
Aliasi	CAPN1
Vanjski ID-jevi	OMIM: 114220 MGI: 88263 HomoloGene: 3800 GeneCards: CAPN1
Lokacija gena (čovjek)
Hrom.	Hromosom 11 (čovjek)
Kraj	65,212,006 bp
Lokacija gena (miš)
Hrom.	Hromosom 19 (miš)
Kraj	6,065,927 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• cysteine-type peptidase activity; • vezivanje iona metala; • GO:0070122 peptidase activity; • GO:0001948, GO:0016582 vezivanje za proteine; • hydrolase activity; • endopeptidase activity; • calcium ion binding; • calcium-dependent cysteine-type endopeptidase activity;
Ćelijska komponenta	• membrana; • focal adhesion; • intracellular anatomical structure; • mitohondrija; • Lizozom; • Egzosom; • citosol; • extracellular region; • citoplazma; • ćelijska membrana; • ficolin-1-rich granule lumen;
Biološki proces	• extracellular matrix disassembly; • receptor catabolic process; • positive regulation of cell population proliferation; • regulation of macroautophagy; • mammary gland involution; • neutrophil degranulation; • GO:0048552 regulation of catalytic activity; • cornification; • self proteolysis; • Proteoliza; • regulation of NMDA receptor activity;
	Izvori:Amigo / QuickGO
Ortolozi
	823
	12333
	ENSG00000014216
	ENSMUSG00000024942
	P07384
	O35350
	NM_001198868; NM_001198869; NM_005186
	NM_001110504; NM_007600
	NP_001185797; NP_001185798; NP_005177
	NP_001103974; NP_031626
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Katalitska podjedinica kalpaina-1 (CANP 1) jest protein koji je kod ljudi kodiran genom CAPN1 sa hromosoma 11.^[5]^[6]^[7]

Aminokiselinska sekvenca[uredi | uredi izvor]

Dužina polipeptidnog lanca je 714 aminokiselina, a molekulska težina 81.890 Da.^[7]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MSEEIITPVY	CTGVSAQVQK	QRARELGLGR	HENAIKYLGQ	DYEQLRVRCL
QSGTLFRDEA	FPPVPQSLGY	KDLGPNSSKT	YGIKWKRPTE	LLSNPQFIVD
GATRTDICQG	ALGDCWLLAA	IASLTLNDTL	LHRVVPHGQS	FQNGYAGIFH
FQLWQFGEWV	DVVVDDLLPI	KDGKLVFVHS	AEGNEFWSAL	LEKAYAKVNG
SYEALSGGST	SEGFEDFTGG	VTEWYELRKA	PSDLYQIILK	ALERGSLLGC
SIDISSVLDM	EAITFKKLVK	GHAYSVTGAK	QVNYRGQVVS	LIRMRNPWGE
VEWTGAWSDS	SSEWNNVDPY	ERDQLRVKME	DGEFWMSFRD	FMREFTRLEI
CNLTPDALKS	RTIRKWNTTL	YEGTWRRGST	AGGCRNYPAT	FWVNPQFKIR
LDETDDPDDY	GDRESGCSFV	LALMQKHRRR	ERRFGRDMET	IGFAVYEVPP
ELVGQPAVHL	KRDFFLANAS	RARSEQFINL	REVSTRFRLP	PGEYVVVPST
FEPNKEGDFV	LRFFSEKSAG	TVELDDQIQA	NLPDEQVLSE	EEIDENFKAL
FRQLAGEDME	ISVKELRTIL	NRIISKHKDL	RTKGFSLESC	RSMVNLMDRD
GNGKLGLVEF	NILWNRIRNY	LSIFRKFDLD	KSGSMSAYEM	RMAIESAGFK
LNKKLYELII	TRYSEPDLAV	DFDNFVCCLV	RLETMFRFFK	TLDTDLDGVV
TFDLFKWLQL	TMFA

Funkcija[uredi | uredi izvor]

Kalpaini, neutralne proteaze aktivirane kalcijem, su nelizozomalne, unutarćelijskee cisteinskr proteaze. Kalpaini sisara uključuju sveprisutne proteine specifične za želudac i mišiće. Sveprisutni enzimi se sastoje od heterodimera s različitim velikim, katalitskim podjedinicama povezanim sa zajedničkom malom, regulatornom podjedinicom. Ovaj gen kodira veliku podjedinicu sveprisutnog enzima, kalpain-1.^[7]

Interakcije[uredi | uredi izvor]

Pokazalo se da CAPN1 reaguje sa PSEN2.^[8]

References[uredi | uredi izvor]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000014216 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000024942 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Aoki K, Imajoh S, Ohno S, Emori Y, Koike M, Kosaki G, Suzuki K (Oct 1986). "Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence". FEBS Lett. 205 (2): 313–7. doi:10.1016/0014-5793(86)80919-X. PMID 3017764. S2CID 36697759.
^ Ohno S, Minoshima S, Kudoh J, Fukuyama R, Shimizu Y, Ohmi-Imajoh S, Shimizu N, Suzuki K (Nov 1990). "Four genes for the calpain family locate on four distinct human chromosomes". Cytogenet Cell Genet. 53 (4): 225–9. doi:10.1159/000132937. PMID 2209092.
^ ^a ^b ^c "Entrez Gene: CAPN1 calpain 1, (mu/I) large subunit".
^ Shinozaki K, Maruyama K, Kume H, Tomita T, Saido TC, Iwatsubo T, Obata K (1998). "The presenilin 2 loop domain interacts with the mu-calpain C-terminal region". Int. J. Mol. Med. 1 (5): 797–9. doi:10.3892/ijmm.1.5.797. PMID 9852298.

Dopunska literatuta[uredi | uredi izvor]

Suzuki K, Sorimachi H, Yoshizawa T, Kinbara K, Ishiura S (1996). "Calpain: novel family members, activation, and physiologic function". Biol. Chem. Hoppe-Seyler. 376 (9): 523–9. doi:10.1515/bchm3.1995.376.9.523. PMID 8561910.
Huang Y, Wang KK (2001). "The calpain family and human disease". Trends in Molecular Medicine. 7 (8): 355–62. doi:10.1016/S1471-4914(01)02049-4. PMID 11516996.
Goll DE, Thompson VF, Li H, Wei W, Cong J (2003). "The calpain system". Physiol. Rev. 83 (3): 731–801. doi:10.1152/physrev.00029.2002. PMID 12843408.
Banik NL, DeVries GH, Neuberger T, Russell T, Chakrabarti AK, Hogan EL (1991). "Calcium-activated neutral proteinase (CANP; calpain) activity in Schwann cells: immunofluorescence localization and compartmentation of mu- and mCANP". J. Neurosci. Res. 29 (3): 346–54. doi:10.1002/jnr.490290310. PMID 1656060. S2CID 40120102.
Sorimachi H, Ohmi S, Emori Y, Kawasaki H, Saido TC, Ohno S, Minami Y, Suzuki K (1990). "A novel member of the calcium-dependent cysteine protease family". Biol. Chem. Hoppe-Seyler. 371 Suppl: 171–6. PMID 2400579.
Harris AS, Croall DE, Morrow JS (1988). "The calmodulin-binding site in alpha-fodrin is near the calcium-dependent protease-I cleavage site". J. Biol. Chem. 263 (30): 15754–61. doi:10.1016/S0021-9258(19)37652-5. PMID 2844821.
Ishiguro H, Higashiyama S, Namikawa C, Kunimatsu M, Takano E, Tanaka K, Ohkubo I, Murachi T, Sasaki M (1987). "Interaction of human calpains I and II with high molecular weight and low molecular weight kininogens and their heavy chain: mechanism of interaction and the role of divalent cations". Biochemistry. 26 (10): 2863–70. doi:10.1021/bi00384a030. PMID 3038169.
Morishita R, Nakayama H, Isobe T, Matsuda T, Hashimoto Y, Okano T, Fukada Y, Mizuno K, Ohno S, Kozawa O (1996). "Primary structure of a gamma subunit of G protein, gamma 12, and its phosphorylation by protein kinase C". J. Biol. Chem. 270 (49): 29469–75. doi:10.1074/jbc.270.49.29469. PMID 7493986.
Kavita U, Mizel SB (1996). "Differential sensitivity of interleukin-1 alpha and -beta precursor proteins to cleavage by calpain, a calcium-dependent protease". J. Biol. Chem. 270 (46): 27758–65. doi:10.1074/jbc.270.46.27758. PMID 7499244.
Du X, Saido TC, Tsubuki S, Indig FE, Williams MJ, Ginsberg MH (1995). "Calpain cleavage of the cytoplasmic domain of the integrin beta 3 subunit". J. Biol. Chem. 270 (44): 26146–51. doi:10.1074/jbc.270.44.26146. PMID 7592818.
Bradford HN, Jameson BA, Adam AA, Wassell RP, Colman RW (1994). "Contiguous binding and inhibitory sites on kininogens required for the inhibition of platelet calpain". J. Biol. Chem. 268 (35): 26546–51. doi:10.1016/S0021-9258(19)74347-6. PMID 8253784.
Oda A, Ozaki K, Druker BJ, Miyakawa Y, Miyazaki H, Handa M, Morita H, Ohashi H, Ikeda Y (1996). "p120c-cbl is present in human blood platelets and is differentially involved in signaling by thrombopoietin and thrombin". Blood. 88 (4): 1330–8. doi:10.1182/blood.V88.4.1330.bloodjournal8841330. PMID 8695851.
Zhang W, Lane RD, Mellgren RL (1996). "The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells". J. Biol. Chem. 271 (31): 18825–30. doi:10.1074/jbc.271.31.18825. PMID 8702541.
Courseaux A, Grosgeorge J, Gaudray P, Pannett AA, Forbes SA, Williamson C, Bassett D, Thakker RV, Teh BT, Farnebo F, Shepherd J, Skogseid B, Larsson C, Giraud S, Zhang CX, Salandre J, Calender A (1997). "Definition of the minimal MEN1 candidate area based on a 5-Mb integrated map of proximal 11q13. The European Consortium on Men1, (GENEM 1; Groupe d'Etude des Néoplasies Endocriniennes Multiples de type 1)". Genomics. 37 (3): 354–65. doi:10.1006/geno.1996.0570. PMID 8938448.
Corasaniti MT, Navarra M, Catani MV, Melino G, Nisticò G, Finazzi-Agrò A (1997). "NMDA and HIV-1 coat protein, GP120, produce necrotic but not apoptotic cell death in human CHP100 neuroblastoma cultures via a mechanism involving calpain". Biochem. Biophys. Res. Commun. 229 (1): 299–304. doi:10.1006/bbrc.1996.1796. PMID 8954122.
Stabach PR, Cianci CD, Glantz SB, Zhang Z, Morrow JS (1997). "Site-directed mutagenesis of alpha II spectrin at codon 1175 modulates its mu-calpain susceptibility". Biochemistry. 36 (1): 57–65. doi:10.1021/bi962034i. PMID 8993318.
Norris FA, Atkins RC, Majerus PW (1997). "Inositol polyphosphate 4-phosphatase is inactivated by calpain-mediated proteolysis in stimulated human platelets". J. Biol. Chem. 272 (17): 10987–9. doi:10.1074/jbc.272.17.10987. PMID 9110986.

Vanjski linkovi[uredi | uredi izvor]

The MEROPS online database for peptidases and their inhibitors: C02.001 Arhivirano 15. 1. 2008. na Wayback Machine
Lokacija ljudskog genoma CAPN1 i stranica sa detaljima o genu CAPN1 u UCSC Genome Browseru.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000014216 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000024942 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid3017764-5] Aoki K, Imajoh S, Ohno S, Emori Y, Koike M, Kosaki G, Suzuki K (Oct 1986). "Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence". FEBS Lett. 205 (2): 313–7. doi:10.1016/0014-5793(86)80919-X. PMID 3017764. S2CID 36697759.

[pmid2209092-6] Ohno S, Minoshima S, Kudoh J, Fukuyama R, Shimizu Y, Ohmi-Imajoh S, Shimizu N, Suzuki K (Nov 1990). "Four genes for the calpain family locate on four distinct human chromosomes". Cytogenet Cell Genet. 53 (4): 225–9. doi:10.1159/000132937. PMID 2209092.

[entrez-7] "Entrez Gene: CAPN1 calpain 1, (mu/I) large subunit".

[pmid9852298-8] Shinozaki K, Maruyama K, Kume H, Tomita T, Saido TC, Iwatsubo T, Obata K (1998). "The presenilin 2 loop domain interacts with the mu-calpain C-terminal region". Int. J. Mol. Med. 1 (5): 797–9. doi:10.3892/ijmm.1.5.797. PMID 9852298.

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p r u Proteaza: Cisteinska proteaza (EC 3.4.22)
Kaspaza	Kaspaza-1 Kaspaza-2 Kaspaza-3 Kaspaza-4 Kaspaza-5 Kaspaza-6 Kaspaza-7 Kaspaza-8 Kaspaza-9 Kaspaza-10 Kaspaza-12 Kaspaza-13 Kaspaza-14
Izvedene iz plodova	Papain Fikain Bromelain Aktinidain
Kalpain	CAPN1 CAPN2 CAPN3 CAPN5 CAPN6 CAPN7 CAPN8 CAPN9 CAPN10 CAPN11 CAPN12 CAPN13 CAPN14 CAPNS1 CAPNS2
Katepsin	B C F H K L1/L2 O S W Z
Ostale	Klostripain Kancerski prokoagulant Separaza Autofagin Kruzipain 3C-lika proteaza