PCMT1
Protein-L-izoaspartat (D-aspartat) O-metiltransferaza jest enzim koji je kod ljudi kodiran genom PCMT1.[3][4][5]
Aminokiselinska sekvenca[uredi | uredi izvor]
Dužina polipeptidnog lanca je 227 aminokiselina, а molekulska težina 24.636 Da.[6]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAWKSGGASH | SELIHNLRKN | GIIKTDKVFE | VMLATDRSHY | AKCNPYMDSP | ||||
| QSIGFQATIS | APHMHAYALE | LLFDQLHEGA | KALDVGSGSG | ILTACFARMV | ||||
| GCTGKVIGID | HIKELVDDSV | NNVRKDDPTL | LSSGRVQLVV | GDGRMGYAEE | ||||
| APYDAIHVGA | AAPVVPQALI | DQLKPGGRLI | LPVGPAGGNQ | MLEQYDKLQD | ||||
| GSIKMKPLMG | VIYVPLTDKE | KQWSRWK |
Funkcija[uredi | uredi izvor]
Tri klase proteinskih karboksil metiltransferaza, koje se razlikuju po specifičnosti supstrata metil-akceptora, pronađene su u prokariotskim i eukariotskim ćelijama. Enzim tipa II katalizira prijenos metilne grupe iz S-adenozil-L-metionina u slobodne karboksilne grupe ostataka D-aspartila i L-izoaspartila. Ovi ostaci koji prihvataju metil, rezultat su spontane deamidacije, izomerizacije i racemizacije normalnih L-asparatilnih i L-asparaginilnih ostataka i predstavljaju mjesta kovalentnog oštećenja proteina koji stare. PCMT1 (EC 2.1.1.77) je enzim za obnovu proteina koji započinje pretvorbu abnormalnih D-aspartyl i L-izoaspartil ostataka u normalni L-aspartil oblik (prema OMIMu).[5]
Reference[uredi | uredi izvor]
- ^ a b c GRCh38: Ensembl release 89: ENSG00000120265 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ MacLaren DC, O'Connor CM, Xia YR, Mehrabian M, Klisak I, Sparkes RS, Clarke S, Lusis AJ (Feb 1993). "The L-isoaspartyl/D-aspartyl protein methyltransferase gene (PCMT1) maps to human chromosome 6q22.3-6q24 and the syntenic region of mouse chromosome 10". Genomics. 14 (4): 852–6. doi:10.1016/S0888-7543(05)80104-1. PMID 1478665.
- ^ DeVry CG, Clarke S (Jun 1999). "Assignment of the protein L-isoaspartate (D-aspartate) O-methyltransferase gene (PCMT1) to human chromosome bands 6q24→q25 with radiation hybrid mapping". Cytogenet Cell Genet. 84 (1–2): 130–1. doi:10.1159/000015239. PMID 10343128. S2CID 38976877.
- ^ a b "Entrez Gene: PCMT1 protein-L-isoaspartate (D-aspartate) O-methyltransferase".
- ^ "UniProt, P22061" (jezik: engleski). Pristupljeno 10. 10. 2021.
Dopunska literatura[uredi | uredi izvor]
- MacLaren DC, Kagan RM, Clarke S (1992). "Alternative splicing of the human isoaspartyl protein carboxyl methyltransferase RNA leads to the generation of a C-terminal -RDEL sequence in isozyme II". Biochem. Biophys. Res. Commun. 185 (1): 277–83. doi:10.1016/S0006-291X(05)80987-8. PMID 1339271.
- Ingrosso D, Kagan RM, Clarke S (1991). "Distinct C-terminal sequences of isozymes I and II of the human erythrocyte L-isoaspartyl/D-aspartyl protein methyltransferase". Biochem. Biophys. Res. Commun. 175 (1): 351–8. doi:10.1016/S0006-291X(05)81242-2. PMID 1998518.
- Ingrosso D, Fowler AV, Bleibaum J, Clarke S (1989). "Sequence of the D-aspartyl/L-isoaspartyl protein methyltransferase from human erythrocytes. Common sequence motifs for protein, DNA, RNA, and small molecule S-adenosylmethionine-dependent methyltransferases". J. Biol. Chem. 264 (33): 20131–9. doi:10.1016/S0021-9258(19)47228-1. PMID 2684970.
- Gilbert JM, Fowler A, Bleibaum J, Clarke S (1988). "Purification of homologous protein carboxyl methyltransferase isozymes from human and bovine erythrocytes". Biochemistry. 27 (14): 5227–33. doi:10.1021/bi00414a042. PMID 3167043.
- Ota IM, Gilbert JM, Clarke S (1988). "Two major isozymes of the protein D-aspartyl/L-isoaspartyl methyltransferase from human erythrocytes". Biochem. Biophys. Res. Commun. 151 (3): 1136–43. doi:10.1016/S0006-291X(88)80484-4. PMID 3355545.
- Takeda R, Mizobuchi M, Murao K, et al. (1995). "Characterization of three cDNAs encoding two isozymes of an isoaspartyl protein carboxyl methyltransferase from human erythroid leukemia cells". J. Biochem. 117 (4): 683–5. doi:10.1093/jb/117.2.267. PMID 7592526.
- Tsai W, Clarke S (1994). "Amino acid polymorphisms of the human L-isoaspartyl/D-aspartyl methyltransferase involved in protein repair". Biochem. Biophys. Res. Commun. 203 (1): 491–7. doi:10.1006/bbrc.1994.2209. PMID 8074695.
- DeVry CG, Tsai W, Clarke S (1997). "Structure of the human gene encoding the protein repair L-isoaspartyl (D-aspartyl) O-methyltransferase". Arch. Biochem. Biophys. 335 (2): 321–32. CiteSeerX 10.1.1.630.8527. doi:10.1006/abbi.1996.0513. PMID 8914929.
- DeVry CG, Clarke S (1999). "Polymorphic forms of the protein L-isoaspartate (D-aspartate) O-methyltransferase involved in the repair of age-damaged proteins". J. Hum. Genet. 44 (5): 275–88. doi:10.1007/s100380050161. PMID 10496068.
- Ryttersgaard C, Griffith SC, Sawaya MR, et al. (2002). "Crystal structure of human L-isoaspartyl methyltransferase". J. Biol. Chem. 277 (12): 10642–6. doi:10.1074/jbc.M200229200. PMID 11792715.
- Smith CD, Carson M, Friedman AM, et al. (2002). "Crystal structure of human L-isoaspartyl-O-methyl-transferase with S-adenosyl homocysteine at 1.6-Å resolution and modeling of an isoaspartyl-containing peptide at the active site". Protein Sci. 11 (3): 625–35. doi:10.1110/ps.37802. PMC 2373461. PMID 11847284.
- Misra P, Qi C, Yu S, et al. (2002). "Interaction of PIMT with transcriptional coactivators CBP, p300, and PBP differential role in transcriptional regulation". J. Biol. Chem. 277 (22): 20011–9. doi:10.1074/jbc.M201739200. PMID 11912212.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Enünlü I, Pápai G, Cserpán I, et al. (2003). "Different isoforms of PRIP-interacting protein with methyltransferase domain/trimethylguanosine synthase localizes to the cytoplasm and nucleus". Biochem. Biophys. Res. Commun. 309 (1): 44–51. doi:10.1016/S0006-291X(03)01514-6. PMID 12943661.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Zhu H, Yang W, Lu W, et al. (2006). "A known functional polymorphism (Ile120Val) of the human PCMT1 gene and risk of spina bifida". Mol. Genet. Metab. 87 (1): 66–70. doi:10.1016/j.ymgme.2005.09.008. PMC 2947858. PMID 16256389.
- Lanthier J, Desrosiers RR (2007). "Regulation of protein L-isoaspartyl methyltransferase by cell-matrix interactions: involvement of integrin alphavbeta3, PI 3-kinase, and the proteasome". Biochem. Cell Biol. 84 (5): 684–94. doi:10.1139/o06-055. PMID 17167531.
- Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.